Labshake search
Citations for Lucigen :
551 - 600 of 1171 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... coli genomic DNA was purified using the MasterPure Gram Positive DNA Purification Kit (Epicentre). All DNAs were purified again using the Zymo Clean and Concentrator-25 (Zymo Research ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing libraries were generated using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) using 12 PCR cycles for amplification ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was prepared using the MasterPure complete DNA and RNA purification kit (Epicentre) and treated with MmeI and the calf intestinal phosphatase (NEB ...
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bioRxiv - Molecular Biology 2019Quote: Yeast total RNA samples were prepared by MasterPure Yeast RNA extraction kit (Lucigen, MPY03100). For mouse ESCs cells ...
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bioRxiv - Genomics 2020Quote: ... Yeast total RNA was extracted with the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... BACs were isolated from 50ml bacterial cultures using the BACMAX Bac extraction kit (Epicentre). Isolated BACs were sonicated (x10 cycles of 15 seconds ‘on’ 45 seconds ‘off’ at constant intensity with power set to ‘3’ ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the Ribo-Zero Gold kit (Epicentre). Ribosome-depleted RNA was used to create multiplexed RNA-seq libraries (NEBNext Ultra ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from candidate mutants with the MasterPure DNA purification kit (Epicentre) and PCR followed by gel electrophoresis confirmed correct integration of the NAT dominant resistance marker ...
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bioRxiv - Molecular Biology 2020Quote: ... and ribosomal RNA was depleted using the Ribo-Zero Plant rRNA removal kit (Epicentre). chrRNA-seq libraries were constructed from 50 ng of purified RNA using the KAPA Stranded mRNA-seq Kit ...
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bioRxiv - Microbiology 2019Quote: ... aureus samples by following manufacturer’s instructions (MasterPure gram positive DNA Purification Kit; Lucigen, USA). Briefly ...
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bioRxiv - Genetics 2019Quote: ... Total RNAs were depleted of ribosomal RNA with Ribo-Zero rRNA Removal kit (Epicentre). Libraries for sequencing ...
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bioRxiv - Immunology 2020Quote: ... The bisulfite-modified DNA-sequencing library was generated using the EpiGnome™ kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Residual Ribosomal RNA (rRNA) was depleted using Ribo-Zero™ kits (Epicentre, Madison, WI) before library construction ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was prepared using the MasterPure™ Yeast DNA purification kit from Lucigen. To determine the MAT ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA was extracted using MasterPure Complete DNA & RNA Purification Kits (Lucigen Simplifying Genomics, USA) according to the manufacturer’s protocol and diluted to 50 ng/μL.
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bioRxiv - Genetics 2020Quote: ... Total RNA was extracted using a MasterPure™ Yeast RNA Purification Kit (Lucigen MPY03100), the RNA concentration was determined on a Nanodrop 2000C (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... respectively) had their total genomic DNA extracted using MasterPure Yeast DNA Purification Kit (Lucigen), with cell lysis solutions and RNAse A treatment volumes scaled up 4x to adequately lyse all cells and remove RNA ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA library was prepared using a NxSeq AmpFREE Low DNA Library kit (Lucigen) and sequenced on a Illumina HiSeq2000 with a 100bp paired-end mode ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were prepared using the ScriptSeq™ Complete Kit (Epicentre, Madison, WI, USA) according to the manu-facturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA extraction from pellets was done using MasterPure™ DNA purification kit (Epicentre, US) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA was depleted using the Ribo-Zero™ Kit (Epicentre, Madison, WI, USA). The viral dsRNA of these strains was extracted and prepared as previously described [22] ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted using Lucigen Kit as per manufacturer’s instructions (Bioresearch, Lucigen, UK) and samples were sequenced by Novagene (Cambridge ...
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bioRxiv - Microbiology 2022Quote: ... Preparation of genomic DNA used the MasterPure™ Yeast DNA Purification Kit (Lucigen # MPY80200) with a NextSeq500– 1flowcell mid output (130M fragments) ...
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bioRxiv - Neuroscience 2023Quote: ... The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre). The 100 μl of clarified lysates were used for total RNA extraction and purification ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted using Epicentre MasterPure yeast RNA purification kit (Epicentre, Madison, WI) according to manufacturer’s instructions and stored in RNase-free Tris-EDTA buffer ...
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using the MasterPureTM Complete DNA and RNA Purification Kit (Lucigen, USA), including an additional bead-beating step using a FastPrep-24TM ...
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bioRxiv - Molecular Biology 2023Quote: ... in vitro transcription reactions were performed using the Ampliscribe T7 flash transcription kit (Lucigen) in a 40 µL reaction with 50 ng of DNA template ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). Quantitative PCR (qPCR ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was purified using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using Lucigen MasterPure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). DNA concentration was measured by bio-spectrophotometer absorbance readings and diluted to desired target concentrations.
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bioRxiv - Bioengineering 2023Quote: ... We synthesized mRNA using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, catalog #ASF-3507) as described previously.(79 ...
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bioRxiv - Developmental Biology 2024Quote: ... The dsRNA was synthesized using the AmpliScribe T7 High Yield Transcription Kit (Lucigen, USA). Primer sequences for the PCR amplification are provided in Supplementary Table S3.
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bioRxiv - Genetics 2024Quote: ... Total DNA was purified using Epicentre Masterpure™ Yeast DNA Purification Kit (Lucigen, USA) and resuspended in 50 µL of RNase-free water ...
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bioRxiv - Microbiology 2024Quote: ... rRNA depletion was accomplished with the Ribo-Zero rRNA Removal Kit (Epicentre, Madison WI) and RNA purity and concentration were confirmed using a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Genomics 2020Quote: ... electroporated in triplicate into Endura electrocompetent cells (Lucigen, Middleton WI), and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Microbiology 2021Quote: ... The vector was transformed into BigEasy-TSA Electrocompetent Cells (Lucigen) for propagation ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was electroporated into Endura competent cells (Lucigen) using 50-100ng DNA per electroporation (we set up 6 or 24 replicate transformation for tiling or genome-wide sgRNA library to maintain library complexity) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the DNA library was transformed into TG1 electrocompetent cells (Lucigen) with an efficiency of approximately 1010 CFU per library before amplification ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed using QuickExtract DNA Extraction Solution (Lucigen) following supplier’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli 10G electrocompetent cells (60080-2, Lucigen, Middleton, WI, US). Cells were selected with appropriate antibiotics on solid and liquid culture ...
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bioRxiv - Microbiology 2021Quote: ... the cell pellet resuspended in 100μL QuickExtract solution (Lucigen #QE09050), transferred to PCR-tubes and incubated in a thermocycler for 15 minutes at 68°C ...
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bioRxiv - Bioengineering 2021Quote: ... Each library was independently electroporated into Endura electrocompetent cells (Lucigen), plated on agar bioassay plates ...
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bioRxiv - Genetics 2019Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Cell Biology 2019Quote: ... Genomic DNA was extracted from HEP3B cells using QuickExtract (Epicentre).
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bioRxiv - Genomics 2022Quote: S2R+ cell genomic DNA was isolated using QuickExtract (QE09050, Lucigen). Fly genomic DNA was isolated by grinding a single fly in 50µl squishing buffer (10 mM Tris-Cl pH 8.2 ...
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bioRxiv - Plant Biology 2022Quote: ... the following plasmids were transformed into BL21DE3-C41 cells (Lucigen): codon optimized pET- DUET1:ZmCPR2/ZmCYP71Z18 or ZmCYP71Z16 ...
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bioRxiv - Genomics 2022Quote: ... Coli cells by electroporation using the manufacturer’s protocol (Lucigen EC300150).