Labshake search
Citations for Lucigen :
551 - 600 of 706 citations for Rat IL 3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The genomic DNA was extracted from homogenized liver tissues using the MasterPure Complete DNA and RNA Purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Total RNAs of nodule and root were processed using the Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA) to deplete ribosomal RNAs ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the bacterial pellets using the MasterPure™ Complete DNA and RNA purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Road Madison, WI). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... ribosomal RNA was depleted from HCT116 wild-type and KO-CREB1 cells by using a Ribo-Zero rRNA Removal Kit (Epicentre). Then ...
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bioRxiv - Immunology 2021Quote: ... heated to 98°C for 5 minutes and cooled to 24°C before use as template for in vitro transcription using the Ampliscribe T7-Flash Transcription Kit (Lucigen). Reactions were allowed to proceed overnight ...
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bioRxiv - Genetics 2021Quote: ... columns and approximately 120–150 ng of DNA was used as template for a T7 in vitro transcription (IVT) reaction (AmpliScribe-T7-Flash transcription kit from Epicentre). In vitro transcribed gRNAs were DNAse-treated using TURBO-DNAse for 20 min at 37 °C and precipitated with sodium acetate/ethanol and resuspended in RNAse and DNAse free water ...
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bioRxiv - Microbiology 2022Quote: ... DNA-Seq library preparation and sequencing were performed by the Genome Quebec Company (Genome Quebec Innovation Center, McGill University, Montreal, Canada) using the Shotgun PCR-free library preparation kit (Lucigen) and the NovaSeq™ 6000 Sequencing system (Illumina® ...
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bioRxiv - Neuroscience 2021Quote: ... 6µl 500X CopyControl Fosmid Autoinduction Solution was also added to induce the fosmid to high copy according to the CopyControl Fosmid Library Production Kits (EPICENTRE). The fosmid was then prepared using the standard Qiagen miniprep kit.
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bioRxiv - Molecular Biology 2020Quote: ... blunt-end ligation into the pZE21 MCS1 expression vector with constitutive promoter pLteo-1 (Lutz and Bujard, 1997) at the HincII site using the Fast Link ligation kit (Epicentre), and iv ...
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bioRxiv - Molecular Biology 2020Quote: ... Single colonies of each suppressor strain were obtained and genomic DNA was prepped using MasturePure(tm) Yeast DNA Purification Kit (Lucigen). The DHR1 ...
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bioRxiv - Genomics 2020Quote: Libraries were prepared from the DNA samples using the NxSeq AmpFREE Low DNA Library kit (Lucigen, Cat no. 14000-2) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: Ribosomal RNA-depleted RNA-seq libraries were prepared from 200-500 ng of total RNA using the low input ScriptSeq Complete Gold Kit (Epicentre). Libraries were sequenced on an Illumina NextSeq 500 with single-end 75 bp reads.
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bioRxiv - Genetics 2019Quote: ... The DNA was prepared and purified by “MasterPure™ Complete DNA and RNA Purification Kit Bulk Reagents” (Epicentre, Wisconsin, USA). The DNA libraries were constructed using the Nextera DNA Flex Library Prep Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... Nematodes were washed individually from each mapping line in approximate equal pellet sizes prior to pooling and genomic DNA extraction using the MasterPure Kit (Epicentre). We sequenced the whole genomes of tu391 and csu60 ...
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bioRxiv - Microbiology 2019Quote: DNA of DBB and MSL71⊤ cells was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, WI, USA). The genomes were sequenced using the Illumina HiSeq2000 paired-end sequencing platform (GATC Biotech ...
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bioRxiv - Microbiology 2019Quote: ... was used to create a fusion PCR product to replace fHbp in strain L91543 with the kanamycin resistance gene (kan) from EZ::Tn5
insertion kit (Epicentre) following the approach described by Horton [57] ... -
bioRxiv - Evolutionary Biology 2019Quote: ... Cells grown on YES solid media were used for genomic DNA preparation using the MasterPure Yeast DNA Purification Kit (Epicentre). The kit manufacturer’s protocol was followed ...
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bioRxiv - Microbiology 2021Quote: DNA isolation from inactivated cells was carried out using MasterPure™ Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Insert-DNA (50 ng) was ligated with vector-DNA (150 ng) using the Fast-Link DNA Ligation Kit (LK0750H, Lucigen). XL10-Gold ultracompetent cells were transformed with the ligation product and streaked out on agar plates containing Kanamycin ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of rRNA-depleted RNA was used to construct sequencing libraries using a ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre). Twelve PCR cycles were used for amplification of the libraries ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA molecules were depleted from the total RNA samples using the Ribo-Zero rRNA Removal Kit for bacteria (Epicentre), and small RNAs (< 200 nt ...
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bioRxiv - Neuroscience 2020Quote: DNA from six PVN from each supplemental tactile stimulation group in the repeated room temperature condition and nest temperature condition (total n = 24) was extracted using the Masterpure Complete DNA/RNA Extraction kit (Epicentre) and 300 ng of DNA was used for bisulfite conversion using the Epitect Fast Bisulfite Conversion kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... GU332722.1) or Asia II 1 (GenBank accession no. GU332721.1) was synthesized using the AmpliScribe T7- Flash Transcription Kit (Epicentre, ASF3507), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from cell pellets as described (Greenstein et al 2018) using the Masterpure yeast RNA extraction kit (Lucigen). cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018 ...
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bioRxiv - Microbiology 2021Quote: ... and further used for the cDNA synthesis by using ScriptSeq™ v2 RNA-Seq kit (Epicentre, Illumina, Madison, WI USA). The cDNA was purified using AMPure XP (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted from the frozen tissues (10 mg) with a MasterPure Complete DNA and RNA purification Kit (Lucigen). We used Q5®High-Fidelity 2X Master Mix (NEB ...
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bioRxiv - Microbiology 2022Quote: ... The resulting air-dried pellets were resuspended in RNase-free water and 3 µg of DNA from each sample was depleted of ribosomal RNA using the Bacteria Ribo-Zero rRNA Removal Kit (Epicentre) and purified using the RNeasy miniprep kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: ... Subsequent library preparation was performed with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen®; Cat. no. 14000-1) according to the manufacturer’s instructions with one slight modification ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg of purified RNA was depleted of ribosomal RNA using a Ribo-Zero rRNA Removal Kit - Plant Leaf (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The library prep was performed according to the manufacturer’s instructions for the NxSeq® AmpFREE Low DNA Library Kit from Lucigen® (Cat No ...
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bioRxiv - Molecular Biology 2020Quote: ... Metatranscriptomic libraries were prepared for sequencing with the addition of 5–50 ng of RNA to the ScriptSeq cDNA V2 library preparation kit (Epicentre). Metatranscriptomic samples were sequenced with an Illumina NextSeq 500 system using V2 high output 300 cycle reagent kit with PHIX control added ...
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bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... A library of ∼500,000 independent mini-Tn5 transposon mutants was created by transforming BW25113 ΔascA with an EZ-Tn5
Tnp transposome kit (Epicentre) by electroporation ... -
bioRxiv - Molecular Biology 2020Quote: ... fragmentation of extracted DNA into pieces of an average size of 2 kb by sonication using a Covaris E210 (Massachusetts, USA) followed by end-repair using the End-It end repair kit (Epicentre), iii ...
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bioRxiv - Molecular Biology 2022Quote: ... Fig 5B-D and Fig EV5 were obtained as follows: Total RNA was extracted from cells using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Epicentre), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Half of the cells in each well were used to purify genomic DNA via the Epicentre MasterPure DNA Purification Kit (Epicentre). PCR was used to amplify the CRISPR reaction locus with designed primer pairs ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1.5 μg of RNA per sample was used as input material for rRNA removal using the Ribo-Zero rRNA Removal Kit (Epicentre). cDNA libraries were generated using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted with Total RNA Zol-Out Kit (A&A Biotechnology, Poland) and converted to cDNA using NxGen M-MulV reverse transcriptase (Lucigen) with random primers (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Microbiology 2020Quote: The DNA isolation procedure and WGS procedure was performed as follows: DNA was extracted using the MasterPure DNA isolation kit (Lucigen) or MasterPure Gram Positive DNA purification kit (Lucigen) ...
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bioRxiv - Microbiology 2021Quote: ... Phage and bacterial genomic DNA were prepared with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2019Quote: A genomic library of Pseudoalteromonas issachenkonii (isolate no. 91) was constructed using the Copy Control Fosmid Library Production Kit and vector pCC1FOS (Epicentre) with the modifications described by Weiland et al ...
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bioRxiv - Genetics 2020Quote: ... and used as a template for in-vitro transcription of gRNA by AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies).
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bioRxiv - Neuroscience 2020Quote: ... RNA samples with RNA integrity numbers (RIN) greater than seven (mean RIN 8.5) were processed with Ribo-Zero Gold kit (Epicentre, WI) to remove ribosomal RNA ...
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Biochemistry 2021Quote: ... pure DNA was isolated by using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Illumina, San Diego, CA, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from harvested cultures using the Masterpure™ Complete DNA and RNA Kit (Lucigen Corporation, Middleton, Wisconsin). RNA was subsequently purified using the Zymo RNA Clean and Concentrator™ Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... or XhoI- (for plasmids with the prefix “pUC57-”) digested plasmids or PCR products using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), followed by capping with ScriptCap m7G Capping System (Cell Script) ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted directly from the sorted nuclei without centrifugation using the MasterPure DNA Extraction kit (Epicentre, Madison, Wisconsin, USA) following the manufacturer’s instructions.