Labshake search
Citations for Lucigen :
601 - 650 of 672 citations for Rat IGF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... shRNAs were synthesized by in vitro transcription (IVT) from double stranded DNA templates using the AmpliScribeTM T7-flashTM transcription kit (Lucigen, Inc.) and were purified using Direct-Zol TM RNA Miniprep kits (Zymo Research ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg of RNA was used as template for reverse transcription following the manufacturer’s instructions (EpiScript™ Reverse Transcriptase System kit, Epicentre, USA), using random hexamer primers (Fermentas ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were then used as templates for in vitro transcription per AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies) specifications to obtain dsRNAs.
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was isolated by chloroform extraction and ethanol precipitation and amplified using TargetAmp™ 1-Round aRNA Amplification Kit 103 (Epicentre) according to vendor protocols ...
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bioRxiv - Genomics 2020Quote: ... Dual extraction of nucleic acid (RNA and DNA) from each pupa was carried out using the MasterPure dual extraction kit (Epicentre, MC85200). Briefly ...
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bioRxiv - Genomics 2019Quote: DNA for ChIP and other high throughput sequencing approaches was processed as follows: End repair was performed using an Epicenter End-It kit (Lucigen ER0720), according to manufacturer’s instructions and column purified in a Zymo Minelute column (Zymo D4013) ...
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bioRxiv - Developmental Biology 2022Quote: ... These solutions (5.5 µl each) were then used as template for a 20 µl reaction with the AmpliScribeTM T7 Transcription Kit (Lucigen, AS3107). The reaction product was treated with DNAseI and shRNAs were purified using the RNA Clean and ConcentratorTM Kit (Zymo Reasearch ...
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bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA from overnight saturated cultures of isogenic bacterial clones was prepared using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) according to the manufacturer’s guidelines and sequenced at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Crude genomic DNA was generated by incubating yeast isolates at 95°C in 50 μL of 20 mM NaOH for 10 minutes or the MasterPure™ Yeast DNA Purification Kit (Lucigen). PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega) ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was repaired with Blunt-end ending 3′ overhang using End-It DNA End-Repair kit in 25 µl volume (Epicentre; ER81050), and 3′-A overhang was added with Klenow fragment (NEB ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were then prepared from 5 ng of DNA by performing end-repair with the End-it DNA End-Repair Kit (Lucigen, ER81050), followed by A tailing with NEB Klenow Fragment (3’−5’ exo- ...
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bioRxiv - Biochemistry 2023Quote: Fosmids from each hit were isolated by BioS&T (Quebec, Canada) or using the FosmidMAX™ DNA Purification Kit (Lucigen Corporation). Isolated fosmids were prepared for Illumina sequencing using the plexWell™ 96 kit (seqWell ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was isolated from yeast and contaminating DNA was depleted using the MasterPure Yeast RNA Purification Kit (Epicentre, Madison, WI) protocol with minor changes as previously described (Carrocci et al. ...
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bioRxiv - Genetics 2023Quote: ... at least 30 5-FOA resistant clones were grown in YEPD for DNA extraction by MasterPure™ Yeast DNA Purification Kit (Lucigen). The relative location of each chromosome truncation event was determined using multiplex PCR with primers that anneal centromere or telomere proximal to the SiRTA (File S1) ...
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bioRxiv - Genetics 2023Quote: ... Thirty clones that displayed growth on medium containing 5-FOA were selected and inoculated in liquid YEPD for genomic DNA extraction using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Multiplex PCR was used as described in Ngo et al ...
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bioRxiv - Microbiology 2023Quote: ... The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4) by blunt-end ligation enzyme (Epicentre FastLinkTM kit) and ligation products in the range of 4-10 kb were extracted from a 0.7% agarose gel.
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bioRxiv - Evolutionary Biology 2023Quote: A transposon mutant library for the WT strain was generated by electroporation using the EZ-Tn5
Tnp transposome kit (Lucigen Inc.). The library consisted of approximately 40,000 insertion mutants ... -
bioRxiv - Microbiology 2023Quote: ... Genomic DNA from isolates that exhibited uracil auxotrophy was purified using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The promoter ...
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bioRxiv - Neuroscience 2024Quote: ... the pGEMHE plasmids carrying different KCR1 variants were linearized by NheI digestion and used for in vitro transcription of complementary RNA (cRNA) using the AmpliCap-Max T7 high-yield message maker kit (Epicentre Biotechnologies). For all the KCR1 expression variants ...
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bioRxiv - Molecular Biology 2021Quote: ... whole genome sequencing was performed on DNA isolated from approximately 30 OD600 yeast cells using Lucigen MasterPure Yeast DNA Purification kit (Lucigen catalog: MMPY80200) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2019Quote: ... held by a 4-axis micromanipulator under bright-field optics and transferred to tubes containing 3uL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 hour of removal from the incubator and within 4 hours of removal from the animals.
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bioRxiv - Neuroscience 2020Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, Message BOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Genetics 2020Quote: The DNA isolation was performed on the swim up purified sperm using the MasterPure DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA) as previously described [13] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reverse complement DNA templates were ordered from Integrated DNA Technologies (USA) and transcribed using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen). The DNase treated products were cleaned with Quick-RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Microbiology 2021Quote: ... and the phage pellet used for phage DNA preparation with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Genomics 2019Quote: ... was removed from 4µl of total RNA of each sample using Epicentre Ribo-zero(tm) rRNA Removal Kit (Epicentre, Madison, WI, USA). The rRNA-depleted RNA was then used to generate strand-specific RNA-seq libraries using TruSeq® Stranded mRNA Kit (Illumina ...
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bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... RNA was sent to sequencing facilities (Macrogen, Seoul, Rep. of Korea): ribosomal RNAs (rRNA) were depleted (Ribo-ZeroTM Gold Kit,Epicentre, Madison, USA), cDNA libraries were produced (TrueSeq totalRNA sample kit ...
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bioRxiv - Genomics 2022Quote: ... from at least two freshly starved 9 cm plates of the appropriate worm strain using the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), following manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: High molecular weight DNA extracted from all enrichment cultures was combined in equimolar amounts and used to prepare two metagenomic fosmid libraries ‘IS_Lib1’ (Cavascura enrichments) and ‘IS_Lib2’ (Maronti enrichments) using the CopyControl™ Fosmid Library pCC2FOS Production Kit (Epicentre Technologies, Madison, USA). DNA was end-repaired to generate blunt-ended 5’-phosphorylated fragments according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... primer sets were screened against a synthetic TSV RNA target in vitro transcribed from gBlock™ DNA (IDT, Table S1) using the Ampliscribe T7-Flash Transcription kit (Lucigen Corporation) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were selected and subcultured in THM to stationary phase and genomic DNA prepared using the Masterpure Gram-positive DNA purification kit (Lucigen, Middleton, WI). The samples then were purified further using ZymoDNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Evolutionary Biology 2023Quote: The shRNA and mimiRs were transcribed according to the manufacturer’s instructions using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen, USA) with a few changes ...
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bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Developmental Biology 2024Quote: ... ribosomal RNA was removed from 3 μg of total RNA per sample using the Epicentre Ribo-zero™ rRNA Removal Kit (pig; Epicentre, USA). Strand-specific libraries were generated from the rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Developmental Biology 2020Quote: We used 200-250 primordial follicles and 100-150 primary follicles for cDNA synthesis using the Message Booster cDNA synthesis kit (Lucigen, Palo Alto, CA). Direct cDNA and subsequent cRNA syntheses were performed by following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... The dsRNAs of target genes were synthesized from the purified PCR products using AmpliScribe T7-Flash Transcription Kit (Epicentre Technologies, Co., Wisconsin, USA).
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bioRxiv - Molecular Biology 2020Quote: ... subtilis lysine riboswitch and Tetrahymena ribozyme were also produced by in vitro transcription using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). All constructs were 5’ capped using vaccinia capping enzyme (NEB-M2080S ...
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bioRxiv - Immunology 2021Quote: 200ul aliquots of blood samples collected on GD15 were used for genomic DNA (gDNA) extraction for bisulfite pyrosequencing using MasterPure DNA Purification kits (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...