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Citations for Lucigen :
51 - 100 of 179 citations for RET V804M IN 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 4 μl of T5-Tn5 transposome and 4 μl of T7-Tn5 transposome were mixed to form 8 μl of assembled Tn5 transposome before it was mixed with 1 μl cDNA (containing ~500 pg cDNA) and 1 μl reaction buffer (TNP92110, Lucigen). The reaction mixture was incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology) ...
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bioRxiv - Genomics 2020Quote: ... 1 ul Hybridase Thermostable RNase H (Lucigen, H39500) and 1 ul 10 X digestion buffer (500 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... using Endura™ ElectroCompetent Cells (Lucigen 60242-1). Resulting pooled DNA libraries were used to produce lentiviral particles and infect primary cortical neurons.
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bioRxiv - Plant Biology 2021Quote: ... coli C41 (DE3) cells (Lucigen, Cat# 60442-1) and purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μL/mL Ready-Lyse Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Biochemistry 2019Quote: ... For RNase III tests 1 unit of enzyme (Epicentre) was added 10 min after addition of RNases I and H ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Electroporations were recovered in 1 mL recovery media (Lucigen) for 1 hour and subsequently grown overnight in LB + selection.
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bioRxiv - Biochemistry 2019Quote: ... and 1 μl of CircLigase I (Epicentre, Madison, WI). Ligation reactions were incubated at 60C for 2 h and the ligase was heat inactivated by incubating at 80C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μL of Endura electrocompetent bacteria (Lucigen 60242-1) were transformed with 150 ng of library DNA using a 0.1 cm Gene Pulser electroporation cuvette (Bio-Rad 1652083 ...
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bioRxiv - Microbiology 2020Quote: ... in combination with 1 µl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... in combination with 1 μl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Immediately after electroporation 1 ml of prewarmed recovery medium (Lucigen, #800261 was added to the bacteria and the cells were incubated for 1 hour at 37 °C shaking at 250 rpm in a bacterial incubator ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Plant Biology 2020Quote: ... A mixture consisting of 1 μl 10x Circligase reaction buffer (Epicentre), 0.5 μl 1 mM ATP ...
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bioRxiv - Genomics 2021Quote: ... 1.5 μl ATP and 1 μl FastLink DNA ligase (Lucigen Corporation), followed by heat inactivation for 15 min at 70°C ...
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bioRxiv - Genomics 2022Quote: ... including a 1:4 ratio of biotin-16-UTP (BU6105H, Lucigen) to UTP at 5 mM ...
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bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by addition of 6 mL of recovery medium (Lucigen, F98226-1) and incubation at 37 °C for one hour at 280 rpm rotation ...
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bioRxiv - Synthetic Biology 2021Quote: ... a highly competent derivative of DH10β was obtained from Lucigen (60107-1)53 ...
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bioRxiv - Neuroscience 2022Quote: ... together with 1 1l genomic DNA in QuickExtract DNA Extraction Solution (Lucigen). After droplet generation with the QX200 Droplet generator (Biorad) ...
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bioRxiv - Biochemistry 2022Quote: ... The electroporated bacteria were then cultured in recovery medium (Lucigen 80026-1) for 1 hour ...
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bioRxiv - Genetics 2019Quote: ... 1 μg of RNA was treated with 20 units of RNAseR (Epicentre) in a 25μl reaction at 37°C for 30 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were grown in E.Cloni 10G Chemically Competent Cells (Lucigen, 60107-1) and were verified by Sanger sequencing (Eton biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 2.5 U of CircLigase II ssDNA Ligase (Lucigen), and incubating the resultant mixtures at 40°C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... Ruptured cells were treated with 1 U/μl OmniCleave endonuclease (Lucigen, OC7850K) for 5 min at 37°C to release the DNA-bound protein pool ...
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bioRxiv - Genetics 2023Quote: ... Final assembled products were delivered to Endura Electrocompetent Cells (Lucigen, 60242-1) via electrotransformation (Bio-Rad Gene Pulser II) ...
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bioRxiv - Genomics 2021Quote: ... and used to electroporate 1-4 tubes of Endura DUO electrocompetent cells (Lucigen) at 1.8 kV distributed over 2 cuvettes (0.1 cm gap width ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) containing 50 U/μL Ready-Lyse Lysozyme (Lucigen #R1804M) and protease inhibitors ...
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bioRxiv - Genetics 2022Quote: Transposon mutagenesis was performed using the EZ-Tn5
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bioRxiv - Microbiology 2021Quote: ... the remainder of samples were lysed by adding 1 ul of ReadyLyse (EpiCentre) and incubating at room temperature for 12 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to end-repair with T4 PNK (Lucigen, 37C for 1 hr). End-repaired RNA was cleaned up with the RNA Clean and Concentrator kit (Zymo) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli genomic DNA in 15 μL of 1× Ampligase DNA Ligase Buffer (Epicentre) containing 250 ng of unshared E ...
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bioRxiv - Genetics 2022Quote: ... The ligated product was then electroporated into Enduratm electro-competent cells (Lucigen #60242-1) in 2 separate reactions ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10G isa highly competent DH10B derivative [54] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 µl from ligation reaction was added onto 25 µl of electrocompetent cells (Lucigen) and cells were transformed by using Electroporator (Bio-Rad MicroPulser ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 1.5 μL of 5 U μL-1 Ampligase Thermostable DNA Ligase (Lucigen, USA). Water was added to a total volume of 25 μL ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...