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Citations for Lucigen :
501 - 550 of 750 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the genomic DNA of each clone was extracted by using QuickExtract solution (Epicentre) and PCR was carried out ...
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bioRxiv - Microbiology 2020Quote: ... was isolated using the MasterPure DNA purification kit (MCD85201 Epicentre, Middleton, WI, USA). For constructing nanB-deletion mutants ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping was achieved by polymerase chain reaction with EconoTaq® DNA polymerase (Lucigen). Primers for genotyping of CEP164 mice ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA-DNA hybrids were specifically digested with Hybridase Thermostable RNase H (Epicentre) at 45 °C for 1 hour ...
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bioRxiv - Genetics 2019Quote: ... The cell pellet was agitated in 100 μL of QuickExtract DNA Solution (Lucigen) to disrupt the pellet and placed in a thermocycler for 15 minutes at 68°C followed by 10 minutes at 95°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining DNA was depleted with 2 U of Baseline-ZERO DNase (Epicentre) and ribosomal RNA was depleted using the Ribo-Zero Plant rRNA removal kit (Epicentre) ...
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bioRxiv - Microbiology 2020Quote: ... or Epicenter MasterPure Complete DNA and RNA Purification Kit (Lucigen, Madison, WI, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), for PacBio sequencing ...
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bioRxiv - Genomics 2021Quote: ... and then were resuspended in 385 µL DNA Quick Extract (Lucigen cat# QE09050) and transferred to a 1.5 mL tube ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA was treated with 0.2 units/ul Plasmid-Safe ATP-Dependent DNase (Epicentre) for 5 days at 37 degrees ...
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bioRxiv - Plant Biology 2022Quote: ... Contaminating DNA was removed from total RNA samples with Baseline-ZERO DNase (Epicentre), whereas ribosomal RNA was removed using a Ribo-Zero rRNA Depletion Kit (Epicentre) ...
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bioRxiv - Neuroscience 2023Quote: ... the genomic DNA was extracted from single clones using QuickExtract solution (Lucigen, QE090500) and HDR was verified by locus specific PCR using DreamTaq DNA Polymerase (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were sorted in 5 μl of QuickExtract DNA Extraction Kit (Epicentre, USA) in 96-well format.
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bioRxiv - Cell Biology 2023Quote: ... and residual linear DNA was degraded by Plasmid-Safe ATP-dependent DNase (Lucigen). In vitro cleavage reactions were performed with 250 ng of Plasmid-Safe-treated circularized DNA ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Microbiology 2023Quote: ... Chromosomal DNA was extracted from the pools using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NxSeq AmpFREE Low DNA Fragment Library Kits (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... blunt-end DNA was prepared using the End-It End Repair Kit (Epicentre), “A” bases were added using Klenow fragment (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was extracted using the MasterPure Complete DNA and RNA Purification Kit (Lucigen). Samples consisted of pools of two individuals per line resulting in 16 flies per each sex- ...
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bioRxiv - Molecular Biology 2023Quote: Remaining linear DNA molecules were digested with Plasmid-Safe ATP-dependent exonuclease (Epicentre) continuously at 37°C for 120 h ...
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bioRxiv - Genetics 2021Quote: ... and libraries were constructed using the NxSeq AmpFREE low DNA Library Kit by Lucigen. Bisulfite conversion was done using the EZ-96 DNA Methylation-GoldTM MagPrep kit (D5042 ...
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bioRxiv - Genomics 2019Quote: ... Remaining linear DNA was removed with Plasmid-Safe-ATP-Dependent DNAse (Epicentre, Madison WI). Guide RNAs were designed using chopchop (http://chopchop.cbu.uib.no/index.php) ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Biopsies were transferred to 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) and lysed as described above ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Molecular Biology 2022Quote: ... 3) Remaining linear DNA was removed by exonuclease (Plasmid-Safe ATP-dependent DNase, Epicentre), assisted by rare-cutting endonuclease MssI (only support Homo sapiens ...
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bioRxiv - Genomics 2022Quote: ... Sonicated gDNA ends were repaired using End-It™ DNA End-Repair Kit (Epicentre) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... which were washed with PBS and resuspended in QuickExtract DNA Extraction Solution (Epicentre, QE09050). Cells were incubated at 68 °C for 15 min and 95 °C for 8 min and the integrated synthetic VL-Ck-2A-VH antibody region was PCR-amplified with flanking primers 5’-CATGTGCCTTTTCAGTGCTTTCTC-3’ and 5’-CTAGATGCCTTTCTCCCTTGACTC-3’ that were specific for the 5’ and 3’ homology arms ...
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bioRxiv - Plant Biology 2020Quote: The LAMP reaction was performed using LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once in PBS and resuspended in 20-50μl QuickExtract DNA Extraction Solution (Lucigen). The reaction mixture was incubated at 65°C for 10 minutes and then 95°C for 2 minutes ...
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bioRxiv - Microbiology 2021Quote: ... All linearized DNA were removed with 0.4 units Plasmid-Safe™ DNase (Lucigen, E3110K) followed by a 1X SPRI beads purification.
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bioRxiv - Microbiology 2019Quote: ... aureus samples by following manufacturer’s instructions (MasterPure gram positive DNA Purification Kit; Lucigen, USA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... The bisulfite-modified DNA-sequencing library was generated using the EpiGnome™ kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Remaining linear DNA was removed by exonuclease (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,) at 37 °C in a heating block and enzyme reaction was carried out continuously for 1 week ...
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bioRxiv - Synthetic Biology 2022Quote: ... The Ampligase Thermostable DNA Ligase for LCR reaction was obtained from Lucigen (Middleton, USA).
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bioRxiv - Synthetic Biology 2022Quote: ... and 1.5 μL of 5 U μL-1 Ampligase Thermostable DNA Ligase (Lucigen, USA). Water was added to a total volume of 25 μL ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted using Lucigen Kit as per manufacturer’s instructions (Bioresearch, Lucigen, UK) and samples were sequenced by Novagene (Cambridge ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using the MasterPureTM Complete DNA and RNA Purification Kit (Lucigen, USA), including an additional bead-beating step using a FastPrep-24TM ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Microbiology 2023Quote: ... Transposomes were prepared using 100 ng transposon DNA and EZ-Tn5 transposase (Lucigen, USA) according to the suppliers specifications ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Biochemistry 2024Quote: ... Genotypes of off-spring were identified by isolating DNA using QuickExtract buffer (Epicentre, #QE09050), subsequent amplification of the targeted area using primers G1 and G2 and employing the T7 endonuclease assay ...
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bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from flash frozen pellets using 30 μl of QuickExtract (Lucigen # QE09050) and samples were heated to 60 °C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... seven to eight small DNA fragments were assembled the pSMART-BAC 2.0 (42030, Lucigen) plasmid backbone using Gibson Assembly (E2621L ...
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bioRxiv - Biochemistry 2024Quote: The mRNA coding for the catalytic mutant AGOs used in the equilibrium binding assay was transcribed in vitro using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), employing the corresponding pBYL-3×FLAG-SUMO-catalytic mutant AGO plasmids linearized with Not I as templates ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg RNA extracted from the specific tethering assays was incubated in a 20 μl reaction volume with 1 unit of Terminator 5’- phosphate-dependent exonuclease (Epicentre) for 60 min at 30°C ...
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bioRxiv - Plant Biology 2019Quote: ... Fractions containing few endogenous bacterial proteins were pooled and treated with SUMO Express Protease (Lucigen, WI) at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was precipitated using ispopropanol and contaminating RNA removed using RiboShredder™ RNase Blend (Epicentre). RNase was removed using an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) ...