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Citations for Lucigen :
151 - 200 of 357 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from the cells using QuickExtract DNA Extract Solution (Lucigen, QE9050). Clones were genotyped by PCR and Sanger Sequencing (Supplementary Fig ...
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bioRxiv - Neuroscience 2023Quote: ... the genomic DNA was extracted from single clones using QuickExtract solution (Lucigen, QE090500) and HDR was verified by locus specific PCR using DreamTaq DNA Polymerase (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from individual colonies was isolated using QuickExtract DNA extraction solution (Lucigen). Then ...
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bioRxiv - Cell Biology 2022Quote: ... extraction of genomic DNA was performed with QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Molecular Biology 2023Quote: DNA from GFP-positive cells was isolated using QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Microbiology 2023Quote: ... 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre, Lucigen, Middleton, WI), 5 μl of 1 mg/ml mutanolysin (M9901 ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from cell pellets with QuickExtract DNA Extraction Solution (Lucigen) via manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The gapfill and ligation solution was composed of 1X Ampligase buffer (Lucigen A3210K), 50 nM dNTPs (New England Biolabs N0447L) ...
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bioRxiv - Bioengineering 2023Quote: Edited cells were harvested and treated with Quick Extraction solution (Epicentre, Madison, WI) to lyse the cells (65 °C for 20 min and then 95 °C for 20 min) ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from clones using QuickExtractTM DNA isolation solution (Lucigen #QE09050). PCRs were performed with KOD hot-start polymerase (Merck #71086) ...
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bioRxiv - Microbiology 2024Quote: ... 100U/ml Omnicleave (Epicentre), and Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2024Quote: ... 100U/ml Omnicleave (Epicentre), and Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng of each sample were PCR amplified using the FailSafe PCR system with PreMix H (Lucigen). Southern blot-based detection of telomere PCR products was modified from previous protocols 48,49 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transposed products were enriched by electroporating approximately 100 ng of plasmid pool into Endura Electrocompetent Cells (Lucigen), which are a non- PIR strain that limits recombination ...
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bioRxiv - Developmental Biology 2021Quote: ... ear clips were lysed by incubation in 25 μl of QuickExtract Solution (QE09050, Lucigen) at 65 °C and shaking at 600 rpm for 30 min ...
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bioRxiv - Neuroscience 2019Quote: Genomic DNA was extracted with 10-13 µl of QuickExtract DNA Extraction Solution (Epicentre). Fragments around the intended mutation sites were amplified by PCR in 50 µl reactions with Taq DNA polymerase (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... Knockout clones were confirmed by genomic DNA extraction using QuickExtract DNA extraction solution (Epicentre), PCR amplification of the target locus (forward primer ...
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bioRxiv - Genomics 2020Quote: The genomic DNA of collected cells was extracted using QuickExtract DNA Extraction Solution (Epicentre). Genomic PCR reaction was performed using 100 ng genomic DNA ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Genomics 2019Quote: ... DNA from surviving clones (194/1200) was isolated using DNA quick extract solution (Lucigen) following a modified protocol ...
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bioRxiv - Microbiology 2019Quote: ... they were incubated in the presence of Ready-Lyse lysozyme solution (Epicentre, Madison, WI) for 10 minutes at 37°C ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Biochemistry 2021Quote: ... from which the genomic DNA was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for subsequent sequencing analysis.
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cell lines using QuickExtract DNA Extraction Solution (Epicentre, #QE09050). Specifically ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA of expanded clones was isolated using QuickExtract DNA extraction solution (Lucigen, QE0905T). PCR was performed by amplifying a 241bp area surrounding the mutation site ...
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bioRxiv - Neuroscience 2021Quote: ... the genomic DNA was extracted using the QuickExtract DNA Extraction solution (Epicentre, Madison, USA), according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2020Quote: ... which were washed with PBS and resuspended in QuickExtract DNA Extraction Solution (Epicentre, QE09050). Cells were incubated at 68 °C for 15 min and 95 °C for 8 min and the integrated synthetic VL-Ck-2A-VH antibody region was PCR-amplified with flanking primers 5’-CATGTGCCTTTTCAGTGCTTTCTC-3’ and 5’-CTAGATGCCTTTCTCCCTTGACTC-3’ that were specific for the 5’ and 3’ homology arms ...
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bioRxiv - Developmental Biology 2022Quote: ... genomic DNA of grown clones was harvested using QuickExtract™ DNA Extraction Solution (Lucigen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once in PBS and resuspended in 20-50μl QuickExtract DNA Extraction Solution (Lucigen). The reaction mixture was incubated at 65°C for 10 minutes and then 95°C for 2 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were prepared using the EasyLyseTM bacterial protein extract solution (Lucigen Corp. USA) or the CelLytic B reagent (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (Lucigen QE0905T), and PCR products containing the site of Cas9 cleavage site were generated using the following primers ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA from single clones was extracted using QuickExtract DNA extraction solution (Lucigen; QE0905T) according to the manufacturer’s suggestion ...
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bioRxiv - Neuroscience 2023Quote: ... Genomic DNA (gDNA) was extracted by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by addition of 50 μl QuickExtract DNA solution (Epicentre, QE09050) to the cells ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from transfected cells using the QuickExtractTM DNA Extraction Solution (Lucigen) and mutations were detected by the SURVEYOR assay (Integrated DNA technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNAs from single clones were extracted using QuickExtract DNA extraction solution (QE0905T; Lucigen), and PCR products containing the site of Cas9 targeting site were generated using the following primers ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA of 3T3 cells was isolated with QuickExtractTM DNA Extraction Solution 1.0 (QE09050, Lucigen). Presence of the homozygous deletion was confirmed by PCR using specific primers ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from puromycin-resistant clones using QuickExtract DNA extraction solution (Lucigen), PCR amplified and tested on agarose gel for accurate deletion.
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bioRxiv - Neuroscience 2024Quote: ... DNA was isolated from ear biopsy samples utilizing QuickExtractTM DNA Extraction Solution (Epicentre, QE09050). The tissue was incubated in 20µl QuickExtractTM at 65°C for 15 minutes ...
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bioRxiv - Physiology 2021Quote: ... mRNA was purified from 100 ng of total RNA by using a Ribo-Zero rRNA removal kit (Epicentre) to deplete ribosomal RNA and convert into double-stranded complementary DNA by using an NEBNext mRNA Second Strand Synthesis Module (E6111L) ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2021Quote: ... we electroporated 1 μL of 25 ng/μL of each library into 50 μL bacteria (Lucigen, 60242-2), with ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...