Labshake search
Citations for Lucigen :
601 - 650 of 797 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... total RNA was subjected to rRNA-depletion using RiboZero™ rRNA Removal Kit (EpiCentre Inc., Madison, WI, USA). Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeq™ v2 RNA-Seq Library Preparation guide (EpiCentre Inc. ...
-
bioRxiv - Genetics 2022Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 μg of RNA was treated with a beta version of Ribo-Zero Magnetic Gold Kit Yeast (Epicentre) to deplete rRNAs ...
-
bioRxiv - Plant Biology 2023Quote: ... 1.5% SDS) and purified by MasterPure Complete DNA and RNA Purification Kit Bulk Reagents (Epicentre, Madison, WI, USA). RNA was prepared by TRizol according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted from yeast pellets using the MasterPure™ Yeast RNA Purification Kit (Lucigen Cat. No. MPY03100) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
-
bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
-
bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
-
A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and derived keratinocytes from three patients (11 total samples) with the MasterPure Complete DNA Purification Kit (Lucigen #MC85200). The gDNA yield was quantified by the Qubit dsDNA high-sensitivity (HS ...
-
bioRxiv - Molecular Biology 2023Quote: ... mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then ...
-
bioRxiv - Bioengineering 2023Quote: ... a PCR-amplified NLuc gene block was transcribed using AmpliScribe™ T7-Flash Transcription Kit (Lucigen, ASF-3507) following manufacturer instructions ...
-
bioRxiv - Microbiology 2024Quote: ... RNA clean-up and library prep were performed using either the Ribo-Zero(TM) rRNA Removal Kit (Epicentre) with the Illumina Truseq Stranded RNA LT kit (Illumina ...
-
bioRxiv - Microbiology 2023Quote: ... and bacterial DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), according to the manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2019Quote: ... were grown overnight at 37°C with 12.5 µg of chloramphenicol and extracted using a BACMAX DNA purification kit (Epicentre). Then ...
-
bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...
-
bioRxiv - Immunology 2021Quote: ... libraries were constructed from first strand cDNA using ScriptSeqTM v2 RNA-Seq library preparation kit (Epicentre Biotechnologies, Madison, WI). Briefly ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... a (modified) CTAB protocol or a rapid desalting method (MasterPure™ Complete DNA and RNA Purification Kit; Lucigen Corporation).
-
bioRxiv - Genomics 2020Quote: ... an aliquot of 25 uL was reserved from each sample for extraction of DNA with MasterPure™ kit (Epicentre) and bacterial cell count was measured using qPCR (TaqMan™) ...
-
bioRxiv - Genomics 2020Quote: ... gDNA for the rest of the strains was extracted using the Masterpure Complete DNA and RNA purification kit (Lucigen) using the protocol for tissue samples ...
-
bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... and shSmt3 GLKD lines was depleted of ribosomal RNA with the Ribo-Zero™ rRNA Removal Kit (Epicentre/Illumina). Initial RNA-seq libraries were made using the TruSeq RNA prep kit by Illumina (shW ...
-
bioRxiv - Microbiology 2019Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed using the Experion RNA Analysis Kit (Bio-Rad ...
-
bioRxiv - Genomics 2021Quote: ... About 30 ug of total gDNA was recovered from using MasterPure™ Complete DNA and RNA Purification kit (Lucigen), and 0.5ug-1ug gDNA was checked on a 1% agarose gel for integrity and quality ...
-
Barcoding and demultiplexing Oxford Nanopore native RNA sequencing reads with deep residual learningbioRxiv - Genomics 2019Quote: ... Curlcake in vitro transcribed (IVT) sequences were produced using the Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). Sequin IVT sequences were produced using SP6 Polymerase (NEB-M0207S) ...
-
bioRxiv - Plant Biology 2021Quote: ... a PCR-free library was prepared with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: Phage DNA extractions were performed from high titer suspensions using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: Stranded RNA-seq libraries were prepared from 1 μg total RNA using ScriptSeq v2 Complete kit for bacteria (Epicentre) and sequenced on Illumina NovaSeq 6000 platform at GENEWIZ ...
-
bioRxiv - Neuroscience 2019Quote: ... 20pg of in vitro transcribed RNA synthesized from the T7 control DNA Template (AmpliScribe™ T7 Transcription Kit, Epicentre) was added to the samples ...
-
bioRxiv - Molecular Biology 2020Quote: The initial RNA library was transcribed from 1µg of input dsDNA using the AmpliScribe T7-Flash Transcription Kit (Epicentre). 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega) ...
-
bioRxiv - Molecular Biology 2022Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Road Madison, WI) with a total amount of 2 ug RNA as an input for each library ...
-
Epigenetics of post-operative delirium: A genome-wide DNA methylation study of neurosurgery patientsbioRxiv - Neuroscience 2022Quote: Genomic DNA was extracted from whole blood tissues with the MasterPure DNA extraction kit (MCD85201, Epicentre, Madison, WI, USA) following the recommended protocol ...
-
bioRxiv - Genomics 2022Quote: DNA was isolated from the 16 parental strains using the Masterpure Yeast DNA Purification Kit (Lucigen, Middleton, WI, USA). The Pacific Biosciences protocol “Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” was used to create libraries from 30 micrograms of DNA ...
-
bioRxiv - Genomics 2022Quote: High molecular weight DNA was extracted using either the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), or by standard phenol/chloroform extraction ...
-
bioRxiv - Immunology 2022Quote: ... and incubated with a rolling-circle amplification (RCA) reaction mixture (1 unit/μL phi29 polymerase (Lucigen, NxGen kit 30221), 1x phi29 polymerase buffer ...
-
bioRxiv - Genomics 2022Quote: Total RNA was extracted from 50mL flash-frozen cell pellets grown to mid-log (OD600 ∼0.65-0.85) using MasterPure™ yeast RNA purification kit (Lucigen) including a DNaseI treatment step ...
-
bioRxiv - Microbiology 2022Quote: ... rRNA was removed from the transcriptome samples using the Ribo-Zero rRNA Removal Kit (Epicentre Biotechnologies; Madison, WI, USA). The quality of the resulting RNA library was assessed on a Bioanalyzer 2100 system (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA (gDNA) was isolated using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre/Lucigen, Middleton, WI, USA) using the DNA purification protocol for bacterial cell samples ...
-
bioRxiv - Genetics 2022Quote: ... genomic DNA of the single cell clones with good proliferation was extracted using a QuickExtract DNA extraction kit (Epicentre). The exon 23-24 region was amplified by a pair of PCR primer (SMCHD1_PCR ...
-
bioRxiv - Bioengineering 2023Quote: ... The genomic DNA was extracted from homogenized liver tissues using the MasterPure Complete DNA and RNA Purification kit (Lucigen) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Isolated DNA was used to construct a fosmid library using the CopyControl™ Fosmid Library Production kit (Lucigen Corporation) following the manufacturer’s instructions.
-
bioRxiv - Genetics 2023Quote: Genomic DNA extraction from homogenized liver tissue was performed using the MasterPure Complete DNA and RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: High molecular weight DNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. Seven days old-mycelia of P ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from the bacterial pellets using the MasterPure™ Complete DNA and RNA purification kit (Epicentre) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Road Madison, WI). Subsequently ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was subjected to ribosomal RNA depletion using the Ribo-Zero Magnetic Gold Kit (Human/Mouse/Rat; Epicentre), according to manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: Total RNAs of nodule and root were processed using the Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA) to deplete ribosomal RNAs ...
-
bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2020Quote: ... ribosomal RNA was depleted from HCT116 wild-type and KO-CREB1 cells by using a Ribo-Zero rRNA Removal Kit (Epicentre). Then ...
-
bioRxiv - Immunology 2021Quote: ... heated to 98°C for 5 minutes and cooled to 24°C before use as template for in vitro transcription using the Ampliscribe T7-Flash Transcription Kit (Lucigen). Reactions were allowed to proceed overnight ...