Labshake search
Citations for Lucigen :
351 - 400 of 682 citations for Nucleic Acid Labeling kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from bacterial cell pellets using MasterPure™ complete DNA purification kit (Lucigen). Oxford Nanopore Minion Sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... 2459 bp) were in vitro transcribed using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). Curlcake 2 was polyadenylated using E ...
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bioRxiv - Plant Biology 2021Quote: ... and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI). RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted according to the manufacturer’s instructions (MasterPure Yeast RNA Purification Kit, Epicentre, MPY03100). Successful plasmid shuffling was confirmed by total RNA extraction and RT-qPCR for rRNA tags ...
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bioRxiv - Microbiology 2022Quote: ... It was then purified using the MasterPure Gram Positive DNA purification kit (Lucigen, Middleton, Wisconsin, USA) using the manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was isolated from PHH using a MasterPure Complete DNA Purification kit (Epicentre, Madison, WI; MC85200) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Directional multiplex libraries were prepared with the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) and paired end sequencing was performed on a Hi-Seq 2000 platform at the Deep Sequencing Core Facility at the University of Massachusetts Medical School in Worchester ...
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bioRxiv - Microbiology 2020Quote: ... 16S rRNA was minimized using the Ribo-Zero rRNA removal kit for bacteria (Epicentre, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on an Illumina HiSeq2500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on the Illumina HiSeq2500 platform (100 bp single-end reads) ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was extracted using the Epicentre Masterpure Complete DNA and RNA Purification Kit (Epicentre/Illumina), following the manufacturer protocol for DNA purification ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from whole adult worms using the MasterPure™ DNA Purification kit (Epicentre Biotechnologies) at the Department of Veterinary Disease Biology (VDB) ...
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bioRxiv - Cell Biology 2021Quote: ... the ribosomal RNA (rRNA) was removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre) with a total amount of 2 ug RNA as an input for each library ...
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bioRxiv - Microbiology 2019Quote: ... rRNA was removed from the total RNA by using ribo-zeroTM magnetic kit (Epicentre®, USA). The rRNA-free RNA was used subsequently for RNA-seq library construction using the TruSeq® RNA LT Sample Prep Kit v2 (Illumina company ...
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bioRxiv - Genomics 2021Quote: ... The libraries were generated using the NxSeq AmpFREE Low DNA Library Kit and NxSeq Adaptors (Lucigen). They were then sequenced on an Illumina HiSeq X (PE150).
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bioRxiv - Cancer Biology 2022Quote: RNA extraction was performed using the MasterpureTM RNA purification kit according to the manufacturer’s instructions (Epicentre). DNase digestion was performed using RQ1 RNase-free DNase (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... End repair of immunoprecipitated DNA was performed using the End-It End-Repair kit (Epicentre, ER81050) and incubating the samples at 25°C for 45 min ...
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bioRxiv - Genomics 2022Quote: ... DNA was purified from these cultures using the MasterPure™ Yeast DNA Purification Kit (Epicenter/Lucigen). Purified DNA was used to amplify each TLO gene using a centromeric chromosome arm specific primer (ALO36-48 and ALO60 ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from triplicate co-cultures with the MasterPure complete DNA purification kit (Lucigen). The proportion of G ...
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bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from whole blood following the MasterPure™ DNA Purification kit (Epicentre, MCD 85201). DNA passing quality control based on NanoDrop spectrometry and in sufficient amount through the Qubit dsDNA Broad Range Assay Kit (ThermoFischer Scientific ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was poly-adenylated by Poly(A) Polymerase Tailing Kit (Epicentre) and depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were thawed and RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) followed by DNase treatment with the DNase TURBO DNA-free kit (Invitrogen AM1907) ...
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bioRxiv - Neuroscience 2023Quote: ... The ribosomal RNA was removed from the purified RNAs using the Ribo-Zero magnetic kit (Epicentre). The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre) ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was done by using the MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) to gain high quality and quantity DNA for metagenomic sequencing ...
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bioRxiv - Systems Biology 2023Quote: ... This pool of elements was end-repaired using End-It DNA End-Repair Kit (#ER0720; Epicentre) and self-ligated using Fast-link ligase (LK0750H ...
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bioRxiv - Genomics 2023Quote: Single-cell DNA extractions were performed using the MasterPure DNA and RNA Purification Kit (Epicentre Biotechnologies) following the protocol as written ...
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bioRxiv - Microbiology 2023Quote: Transposon library of Salmonella was generated using EZ-Tn5
Tnp Transposome kit (Lucigen, TSM99K2) following manufacturer’s instructions ... -
bioRxiv - Molecular Biology 2023Quote: ... RNA samples were depleted of ribosomal RNA with Ribo-ZeroTM Magnetic Gold Kit (cat. # MRZG126 Epicentre). Libraries were constructed on BGI Genomics platform (DNBseq ...
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bioRxiv - Microbiology 2023Quote: Phage DNA was isolated using the MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA) with modifications ...
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bioRxiv - Immunology 2023Quote: Bacterial DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), following the manufacturer’s established protocol ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA (rRNA) was depleted from each RNA sample using the Ribo-ZerorRNA Removal Kit (Epicentre), designed for human ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from the homogenized sample using the MasterPure Complete DNA & RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: A transposon mutant library in pJBG007 was made using EZ-Tn5
Insertion Kit (Lucigen). The following components were used for a 10µl reaction (EZ-Tn5 10X Reaction Buffer (1µl ... -
bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Microbiology 2019Quote: ... Total genomic DNA was extracted from 200 μl of blood using a MasterPure DNA purification kit (Epicentre® Biotechnologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries of ribosome protected fragments were generated using the ARTseq™ Ribosome Profiling Kit (Epicentre, RPHMR12126) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... We first repaired the purified DNA again using the End-it DNA End-Repair Kit (Lucigen #ER0720) following the manufacturer’s suggested conditions ...
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bioRxiv - Genomics 2020Quote: Genomic DNA from 300 million cells was purified using the Master Pure Yeast DNA Purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: The transposon used for genome wide insertion is derived from Ez-Tn5™ custom transposon kits (Epicentre). The insert is a cassette composed of the resistance gene to kanamycin and the gfpmut2 gene under control of the well characterized ribosomal promoter rrnBP1 ...
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bioRxiv - Molecular Biology 2019Quote: 5 µg of extracted RNA was depleted from ribosomal RNA using Ribo-Zero Gold Kit (Epicentre Madison). After fragmentation of the rRNA-depleted RNA ...
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bioRxiv - Genomics 2019Quote: ... coli RNA that underwent reverse transcription and amplification using the ScriptSeq Complete Gold Kit for Epidemiology (Epicentre).
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bioRxiv - Molecular Biology 2020Quote: ... The ‘curlcake’ constructs were in vitro transcribed using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507) with either unmodified rNTPs (UNM) ...
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bioRxiv - Biochemistry 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre, Madison, WI). These pools of barcoded RNAs were converted to Illumina cDNA libraries in three main steps ...
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bioRxiv - Neuroscience 2020Quote: ... mRNAs were purified after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre Biotech., WI). Fluorescently labeled cRNAs derived from these transcripts were used to probe Agilent Mouse V4.0 LncRNA Array containing probes for 22,692 mRNAs (ArrayStar ...
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bioRxiv - Genomics 2020Quote: ... and were used as templates for in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106). The mRNAs were then capped in a subsequent reaction using the ScriptCap m7G Capping System (Cellscript C-SCCE0625).
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bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Microbiology 2020Quote: The genomic DNA from the mycelia was extracted using the MasterPure™ Yeast DNA Purification kit (Lucigen) and used subsequently for PCR amplification ...
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bioRxiv - Evolutionary Biology 2022Quote: ... this mixture was mixed with the components of AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen, USA) and incubated for eight hours at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from 1.5 mL of logarithmically growing cells (OD600 ~0.6-0.8) using the Masterpure Yeast RNA Purification kit (Epicentre). Residual genomic DNA was removed using the Turbo DNA-free kit (Ambion) ...