Labshake search
Citations for Lucigen :
51 - 100 of 130 citations for Mouse RTF1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Remaining linear DNA molecules were digested with Plasmid-Safe ATP-dependent exonuclease (Epicentre) continuously at 37°C for 120 h ...
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bioRxiv - Molecular Biology 2021Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Genomics 2019Quote: ... Remaining linear DNA was removed with Plasmid-Safe-ATP-Dependent DNAse (Epicentre, Madison WI). Guide RNAs were designed using chopchop (http://chopchop.cbu.uib.no/index.php) ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation product was treated with Plasmid-safe nuclease (10,000 units/ml, Epicentre, E3101K) and transformed into SURE2 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... 3) Remaining linear DNA was removed by exonuclease (Plasmid-Safe ATP-dependent DNase, Epicentre), assisted by rare-cutting endonuclease MssI (only support Homo sapiens ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transformed into E.cloni ® 10G Chemically Competent Cells (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All linearized DNA were removed with 0.4 units Plasmid-Safe™ DNase (Lucigen, E3110K) followed by a 1X SPRI beads purification.
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bioRxiv - Genomics 2021Quote: ... Remaining linear DNA was removed by exonuclease (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,) at 37 °C in a heating block and enzyme reaction was carried out continuously for 1 week ...
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bioRxiv - Molecular Biology 2023Quote: ... and treated with a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Molecular Biology 2024Quote: ... These plasmids are produced in strain EC100D pir-116 (from Epicentre Biotechnologies, cat# EC6P0950H) in which the pir-116 allele supports higher copy number of R6K origin plasmids ...
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bioRxiv - Bioengineering 2020Quote: ... PP contamination was removed using the Plasmid Safe ATP-dependent DNase kit (Epicentre, WI, USA), and the products where cleaned and concentrated using the Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... were digested overnight with 10 units of Plasmid-Safe ATP-Dependent DNAse (Lucigen, Middleton, Wisconsin) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the assembled plasmid pool was used to transform 25μl of electrocompetent bacteria (Lucigen #60242-2) on a Bio-Rad Gene-Pulser 2 electroporation system (Bio-Rad # 1652105 ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... The remaining linear DNA was treated by exonuclease (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K) at 37LJ°C for 1 week ...
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bioRxiv - Genetics 2023Quote: ... 400 ng of DNA was incubated with 5 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Biochemistry 2023Quote: ... The remaining nDNA contamination was removed by treatment with Plasmid-Safe ATP-Dependent DNase (Lucigen) to digest linear DNA53 ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were then introduced into BAC-Optimized Replicator v2.0 electrocompetent cells (60210, Lucigen) via electroporation at 2500V ...
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bioRxiv - Systems Biology 2021Quote: ... We performed nine electroporations of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Three hundred nanograms of purified plasmids were electroporated into 25 µl of Endura electrocompetent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid-safe ATP-dependent DNase treatment was carried out according to the manufacturer’s protocol (Epicenter, Lucigen) with using 1 μg of total DNA extracted from SDGC-SEC-purified stress granule cores and 3 units of the enzyme per reaction ...
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bioRxiv - Bioengineering 2019Quote: ... The plugs are then treated overnight with Plasmid-Safe™ ATP-dependent DNase (50U/ ½ plug) from Epicentre and loaded on a standard gel (1% agarose ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transposed products were enriched by electroporating approximately 100 ng of plasmid pool into Endura Electrocompetent Cells (Lucigen), which are a non- PIR strain that limits recombination ...
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bioRxiv - Plant Biology 2021Quote: ... 1 ug of resulting RNased DNA was digested using 1 ul Plasmid Safe ATP-dependent DNase (Lucigen) in a 50 ul reaction for 16 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... linear contaminant DNAs within the crude circular DNA are digested with Plasmid-Safe ATP-dependent DNase (Lucigen) at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The Tn7 integration plasmids with barcode and spectinomycin cassette were extracted from TransforMax EC100D pir + cells (Lucigen) with a Qiagen midi kit ...
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bioRxiv - Genomics 2023Quote: ... Linearized plasmids were used for in vitro transcription with the AmpliScribe T7-Flash Transcription Kit (Lucigen, ASF3507) using 5-Methyluridine-5’-Triphosphate (5-mUTP ...
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bioRxiv - Molecular Biology 2019Quote: ... rRNA was depleted using the Ribo-Zero kit Human/Mouse/Rat (Epicentre), and libraries were prepared using random priming ...
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bioRxiv - Molecular Biology 2020Quote: ... with ribosomal depletion using Human/Mouse/Rat Ribo-Zero kit (Epicentre/Illumina). All samples were sequenced using a 100 base-pair (bp ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic mapping to mouse GRCm38 and RepeatMasker analysis was performed by Lucigen. Read quality was evaluated by Python Bio.SeqIO56 and FastQC57 ...
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bioRxiv - Genomics 2021Quote: ... We performed 9 electroporations in total of the column-purified plasmid into Endura electrocompetent Escherichia coli cells (Lucigen) using a Gene Pulser Xcell (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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bioRxiv - Immunology 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Genetics 2023Quote: ... 15-20 μg of genomic DNA (gDNA) was digested with ∼95 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Genomics 2024Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1.2ug of ligated pUSEPR plasmid DNA per sub-pool was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37°C ...
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bioRxiv - Genomics 2022Quote: ... A total of 1.2 µg of ligated pUSEBR-CRISPR Library plasmid DNA was then electroporated into Endura electrocompetent cells (Lucigen). Competent cells were recovered for 1 h at 37ºC ...
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bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.
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bioRxiv - Genomics 2021Quote: ... adding additional ATP and DNase every 24 h (30 units per day) according to the manufacturer’s protocol (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,). ecDNA-enriched samples were used as template for phi29 polymerase amplification reactions (150043 ...
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bioRxiv - Microbiology 2022Quote: ... T7 promoter (TAATACGACTCACTATAG) followed by the first 1629 nucleotides of the VA1 genome inserted into the pSMART plasmid (Lucigen). A second plasmid ...
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bioRxiv - Genomics 2023Quote: ... The resulting plasmid libraries were purified and concentrated via SPRI bead cleanup before being transformed into electrocompetent cells (Lucigen Endura ...
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bioRxiv - Genomics 2019Quote: ... Gram-negative and Human/mouse/rat Ribo-Zero rRNA Removal Kit (Epicentre Technologies). The resulting RNA was purified using Zymo RNA clean & Contentrator-5 column kit (ZYMO Research) ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strains DLF_R002 and DLF_R003 were constructed as previously described.10 Reporter plasmids were constructed in the pSMART-HC-Kan (Lucigen, WI) backbone ...
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bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligo pools were cloned into a modified pLKO-TET-ON plasmid by the Golden Gate method and the resulting product was used to transform Endura electrocompetent cells (Lucigen) according to the manufacturer’s protocol ...