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Citations for Lucigen :
101 - 130 of 130 citations for Mouse GRK4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from bulk cells transfected with CRISPR/Cas9 plasmids using the epicentre QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Fragments from 500 bp to 1000 bp were excised from an agarose gel and cloned into the pSMART LCKan plasmid (Lucigen) by transforming into competent cells ...
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Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... during which additional ATP and DNase was added every 24LJh (30 units per day) according to the manufacturer’s protocol (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K). The eccDNA samples were cleaned by phenol ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from mouse tails using the QuickExtract DNA extraction kit (Epicentre) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
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bioRxiv - Microbiology 2021Quote: ... OmRV-fragment2/pMA (ampicillin resistant) and pACYC177 low-copy-number plasmids were transformed into 10G chemically competent cells (Lucigen, E. cloni), respectively ...
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bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
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bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
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bioRxiv - Neuroscience 2021Quote: ... in the pET-28 bacterial expression vector together with pLysSRARE2 plasmid (both kindly shared by Dr. Chernomordik, NICHD) were electroporated into ClearColi™ BL21(DE3) according to manufacturer protocol (Lucigen, WI). Bacterial colonies that grew in an LB plate containing 100 μg/ml kanamycin and 30 μg/ml chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
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bioRxiv - Synthetic Biology 2022Quote: ... two ncRNA expression cassettes (for barcoded ncRNAs “A” and “B”) from the Marionette plasmids were cloned into pSol-TSF (Lucigen F843213-1) facing in opposite directions ...
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bioRxiv - Synthetic Biology 2020Quote: ... 22 Alanine production plasmids were constructed using codon optimized (Codon Optimization Tool from the IDT) synthetic DNA and the pSMART-HCKan vector (Lucigen, Middleton, WI). Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
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bioRxiv - Microbiology 2023Quote: ... Digested fragments were cloned into the BamHI/NcoI pre-digested pET15b plasmid using the Fast-Link™ DNA Ligation Kit (Epicentre, Madison, WI), and E ...
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bioRxiv - Molecular Biology 2020Quote: Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Complete cDNAs were sequence-amplified by PCR using KOD One™ PCR Master Mix -Blue- (TOYOBO, Japan) and were cloned into pSMART-LCK plasmid (Lucigen, Middleton, WI, USA) containing a T7 RNA polymerase promoter ...
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bioRxiv - Molecular Biology 2019Quote: ... 5µg of total RNA was subjected to rRNA depletion using the RiboZero Human/Mouse/Rat kit (Epicentre Biotechnologies). cDNA was generated from the depleted RNA using random hexamers or custom primers and Superscript III (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal Kit (Epicentre/Illumina, Human/Mouse/Rat) and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was subjected to ribosomal RNA depletion using the Ribo-Zero Magnetic Gold Kit (Human/Mouse/Rat; Epicentre), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... were sent to BGI (Hongkong) for ribosomal RNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) followed by library construction using non-stranded (Replicate 1 ...
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bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: 10ug of Trizol-extracted RNA from mouse cortex and striatum was treated with Terminator 5’-Phosphate dependent exonuclease (Lucigen TER51020) according to manufacturer’s protocol. ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: One microgram of total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre, Madison, WI, USA) followed by a purification step using AMPure XP Beads (Beckman Coulter ...