Labshake search
Citations for Lucigen :
1 - 50 of 686 citations for Medium Kit without Serum and without Growth Factors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... DNA was extracted directly from the sorted nuclei without centrifugation using the MasterPure DNA Extraction kit (Epicentre, Madison, Wisconsin, USA) following the manufacturer’s instructions.
-
bioRxiv - Genomics 2022Quote: ... oligonucleotide with or without a 5’phosphate or circularized oligonucleotide) were treated with 1 U Terminator exonuclease (Lucigen) or mock treated in the manufacturer’s Buffer A for 1 h at 37 °C followed by 1 h at 30 °C ...
-
bioRxiv - Genomics 2022Quote: Equal amounts of RNA (2µg) were incubated with or without 20 U of Rnase R (RNR07250, Epicentre Biotechnologies) and 40 U of RiboLock RNase inhibitor (EO0381,Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2021Quote: ... 100μg total RNA from cortex or cultured HEK293 cells was treated with or without 1μL of RNaseR [20 U/μl] (Lucigen), plus 1.9μL RNaseOUT [40 U/μL] (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... 30 μg of lysate was diluted to 200 μL in polysome buffer (lysis buffer without Triton X-100) and 1.5 μL RNase I was added (Epicentre #N6901K). RNase digestion was carried out at 200 rpm at 37°C for 45 minutes ...
-
Macrobdella decora: Old World Leech Gut Microbial Community Structure Conserved in a New World LeechbioRxiv - Microbiology 2019Quote: MasterPure complete DNA extraction without column purification into 50μL TE was performed according to manufacturer’s protocol (Epicentre Madison, WI U.S.A.). No significant difference was found between the two extraction methods (PERMANOVA ...
-
bioRxiv - Developmental Biology 2023Quote: ... The whole cassette (HomL-EF1a-TetR-3xFlag-KRAB-SV40pA-NeoR-HomR) with or without KRAB was cloned into the pSMART-HCKAN (Lucigen) vector ...
-
bioRxiv - Developmental Biology 2019Quote: ... and was then incubated for 30 min at 37 °C with or without 5 U μg −1 of RNase R (Epicentre Bio-technologies). Reverse transcription was performed using a QuantiTect Reverse Transcription Kit (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... 500uL of the cultured cells were pelleted and the growth media was removed. DNA was extracted using the Master Pure DNA purification kit (Cat No. 85200) from Epicentre. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L ...
-
bioRxiv - Cancer Biology 2023Quote: ... The electroporated cells were recovered in recovery medium (Lucigen) for 1 h and then plated on Terrific Broth (TB ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 ml of 37 °C Recovery Medium (Lucigen, 80026-1) was added and bacteria were grown in round-bottom tubes for 1 h at 37 °C while shaking at 180 r.p.m ...
-
bioRxiv - Immunology 2023Quote: ... Immediately after electroporation 1 ml of prewarmed recovery medium (Lucigen, #800261 was added to the bacteria and the cells were incubated for 1 hour at 37 °C shaking at 250 rpm in a bacterial incubator ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by addition of 6 mL of recovery medium (Lucigen, F98226-1) and incubation at 37 °C for one hour at 280 rpm rotation ...
-
bioRxiv - Biochemistry 2022Quote: ... The electroporated bacteria were then cultured in recovery medium (Lucigen 80026-1) for 1 hour ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmid Safe DNase kit (EpiCentre) was used to degrade any unspecific recombination products for 30 min at 37 °C ...
-
bioRxiv - Genomics 2019Quote: ... ARTseqTM kit from Epicentre (#RPHMR12126); RNaqueous kit from Ambion (#AM1912) ...
-
bioRxiv - Genetics 2021Quote: ... Masterpure Yeast RNA purification kit (Epicentre) was used to extract the RNA by following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: AmpliScribe SP6 Transcription Kit (Epicentre AS3106).
-
bioRxiv - Microbiology 2019Quote: ... rRNA depletion was performed (rRNA depletion Kit Ribo Zero Magnetic Kit for Gram-positive bacteria; Epicentre [Illumina]) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... Ribo-ZeroTM rRNA Removal Kits (Bacteria) and Ribo-ZeroTM Magnetic Gold Kit (Yeast) (Epicentre, San Diego, CA) were used to remove rRNA from the total RNA ...
-
bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... The culture was diluted again to 400 mL of LB30 medium + 12.5 μg/mL of chloramphenicol + 1x Arabinose induction solution (Lucigen) for overnight ...
-
bioRxiv - Microbiology 2021Quote: ... a combined kit for the ribosomal (rRNA) depletion Ribo-Zero™ Kit (Bacteria) (Epicentre, Illumina, Madison, WI USA) and cDNA library construction kit ...
-
bioRxiv - Genetics 2021Quote: ... ribosomal depleted using Ribo-Zero Kit (Epicentre) and libraries prepared using ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) ...
-
bioRxiv - Systems Biology 2022Quote: ... the ribosomal RNA was depleted by Epicentre Ribo-zero® rRNA Removal Kit (Epicentre). Second ...
-
bioRxiv - Plant Biology 2021Quote: ... A Ribo-Zero magnetic kit (MRZPL116, Epicentre) was used for rRNA depletion from total RNA ...
-
bioRxiv - Immunology 2022Quote: ... ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre) and rRNA-free residue was obtained by ethanol precipitation ...
-
bioRxiv - Microbiology 2022Quote: ATP-Dependent DNase kit (Lucigen, catalog #: E3110K).
-
bioRxiv - Bioengineering 2020Quote: ... using AmpliScribe T7-Flash Transcription Kit (Lucigen) following the manufacturer’s instruction and purified by RNA Clean & Concentrator (Zymo) ...
-
bioRxiv - Genomics 2021Quote: ... and the Globin-Zero Gold kit (Epicentre) to remove globin mRNA and ribosomal RNA ...
-
Multi-omics analysis reveals signatures of selection and loci associated with complex traits in pigsbioRxiv - Genomics 2023Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA), and rRNA- free residue was cleaned up by ethanol precipitation ...
-
bioRxiv - Molecular Biology 2024Quote: ... Poly(A) Polymerase Tailing Kit (Lucigen, UK), Direct RNA Sequencing Kit SQK-RNA002 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 40 μl of the whole cell lysate containing the digested chromatin was taken forward into an overnight blunt end ligation reaction (End-It DNA repair kit and Fast-Link DNA ligation kit, Epicentre) with double stranded DNA adapters at 16°C ...
-
bioRxiv - Genomics 2020Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
-
bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and the libraries have been generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
-
bioRxiv - Genomics 2020Quote: ... and NxSeq AmpFREE Low DNA Library Kit (Lucigen) was applied ...
-
bioRxiv - Genetics 2022Quote: ... and the MasterPureTM Yeast RNA Purification Kit (Epicentre). cDNAs were synthesized with the FastQuant RT Kit (with gDNase ...
-
bioRxiv - Cell Biology 2021Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
-
bioRxiv - Microbiology 2020Quote: ... or MasterPure Gram Positive DNA purification kit (Lucigen). Sequencing libraries were prepared using NexteraXT (Illumina) ...
-
bioRxiv - Cell Biology 2019Quote: ... using the ScriptSeq kit (Epicentre, CA, USA SS10906). Libraries were amplified via polymerase chain reaction for 12–15 cycles and sequenced in two lanes on the HiSeq 2000 platform at BGI Genome Center (Shenzhen ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... repaired with End-IT DNA repair kit (Epicentre), A-tailed with Klenow enzyme (New England Biolabs) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... (ii) Shotgun PCR-free library preparation kit (Lucigen) or (iii ...
-
bioRxiv - Plant Biology 2023Quote: ... with NxSeq AmpFREE Low DNA Library Kits (Lucigen). After size selection by AMPureXP beads ...
-
bioRxiv - Genomics 2022Quote: ... and three libraries for each sex were generated with the NEBNext Ultra RNA library prep kit combined with rRNA depletion via the Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies). Samples were sequenced on an Illumina HiSeq2500 platform at the Vienna BioCenter Core Facilities (VBCF ...
-
bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Ribo Zero kit and samples were converted into Illumina sequencing libraries using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina). Libraries were sequenced using Illumina HiSeq (2 × 150 bp reads) ...
-
bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
-
bioRxiv - Genomics 2019Quote: ... DNA was extracted using “Yeast Masterpure kit” (Epicentre, USA) following the manufacturer’s instructions.
-
bioRxiv - Genetics 2020Quote: Total DNA was extracted using a QuickExtract kit (Lucigen). Unless otherwise indicated ...
-
bioRxiv - Genetics 2020Quote: ... and a MasterPure™ Yeast DNA Purification Kit (Lucigen). PE150 libraries of the evolved strains were prepared and sequenced by Novogen.
-
bioRxiv - Molecular Biology 2020Quote: ... we used the MasterPure RNA purification kit from Epicentre instead of phenol/chloroform extraction to purify RNA after the dephosphorylation and ligation steps.