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Citations for Lucigen :
101 - 150 of 217 citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and electroporated with the Ez-Tn5
2> transposome (Epicentre, Charlotte, North Carolina). Cells recovered at 30°C shaking for 90 minutes ... -
bioRxiv - Molecular Biology 2023Quote: ... one 2 microgram portion received 5 units of RNase R (Lucigen, Middleton, WI) and the other an equal volume of nuclease-free water (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... transposable element insertional mutagenesis using EZ-Tn5™
2> Insertion Kit (Epicentre) and sequencing were performed ... -
bioRxiv - Molecular Biology 2019Quote: One microgram of total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre, Madison, WI, USA) followed by a purification step using AMPure XP Beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... were grown overnight at 37°C with 12.5 µg of chloramphenicol and extracted using a BACMAX DNA purification kit (Epicentre). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... C-circle and G-circles assays were performed as before except that NxGen® phi29 DNA Polymerase (Lucigen Corp.) were used for rolling circle amplifications [25].
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated for 10 min at 37°C in the presence of Ready-Lyse lysozyme solution (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... HBV cccDNA was quantified from total DNA following digestion for 45 min at 37°C with T5 exonuclease (Epicentre) to remove rcDNA followed by 30 min heat inactivation ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37 °C for 5 min.The successful linearization of ecDNA was verified by verifying its sensitivity to exonucleaseATP-dependent DNase (Lucigen). After treatment with exonuclease ...
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bioRxiv - Bioengineering 2022Quote: 2’-Fluoro ssRNA was produced by IVT using the Durascribe T7 IVT kit (Epicentre). Reactions consisted of 0.2-1 μg of purified DNA template per 20 μl reaction ...
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bioRxiv - Microbiology 2019Quote: ... Transposome mixtures were prepared by mixing the EZ-Tn5
2> transposon from Epicentre Biotechnologies ... -
bioRxiv - Evolutionary Biology 2021Quote: ... sample 2 was treated with 20 U RNase R (Epicentre/Illumina, Cat. No. RNR07250) for 1 h at 37°C to degrade linear RNAs ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage of around 200x.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Immunology 2021Quote: ... heated to 98°C for 5 minutes and cooled to 24°C before use as template for in vitro transcription using the Ampliscribe T7-Flash Transcription Kit (Lucigen). Reactions were allowed to proceed overnight ...
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bioRxiv - Biochemistry 2020Quote: ... ratio overnight at 16 °C followed by purification and then electroporation into phage-display competent TG-1 cells (#60502-1, Lucigen). The library was plated onto 2xYT agar plates containing 100 µg/mL carbenicillin and 2% glucose at 37 °C overnight ...
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bioRxiv - Microbiology 2021Quote: The Raman microscopy-selected bacteria were lysed by incubation at 37 °C for 30 minutes with Ready-lyse lysozyme (Epicentre) of 10 U/µl and heat treatment at 95 °C for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... a C-terminal AviTag was added to the pSANG10 expression constructs (pSANG10-Avi) and scFvs transformed into BL21[DE3] cells (Lucigen) containing the pBirAcm plasmid (Avidity).
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bioRxiv - Molecular Biology 2020Quote: ... In vitro transcribed RNA were incubated for 1h at 37°C after addition of 10units DNase I and 10units DNase Zero (Epicentre). The RNAs were then ethanol precipitated and resuspended in water ...
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bioRxiv - Molecular Biology 2020Quote: ... In vitro transcribed RNA were incubated for 1h at 37°C after addition of 10units DNase I and 10units DNase Zero (Epicentre). The RNA were then ethanol precipitated and resuspended in water and dephosphorylated using 2units Alkaline Phosphatase (FastAP ...
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bioRxiv - Systems Biology 2020Quote: ... and 23S rRNA subunits and spaced nine bases apart were then annealed at 65 °C followed by digestion with Hybridase (Lucigen), a thermostable RNase H ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Microbiology 2021Quote: ... flhD or complexed AsflhD with flhD was performed at 37°C in TMN buffer containing 1 µg tRNA for 15 min with RNase III (Epicentre). Samples were loaded on denaturing polyacrylamide gels together with an RNA alkaline ladder as in (75).
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bioRxiv - Genetics 2019Quote: ... or 2µg (25th and 100th generation samples) of RNA were incubated for 1h at 37°C with 5’ RNA polyphosphatase (Epicentre) at a final concentration of 1U/µl ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments in the range of 30-40 kbp were resolved by gel electrophoresis (2 V cm-1 overnight at 4 °C) and recovered from 1% low melting point agarose gel using GELase 50X buffer and GELase enzyme (Epicentre). Nucleic acid fragments were then ligated to the linearized CopyControl pCC2FOS vector following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µg of total RNA was mixed with 3 µl of diluted ERCC RNA spike-in mix and then digested for 1h at 30°C with 1 unit of Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in 1X Reaction Buffer A containing 10 units of SUPERase-In RNase inhibitor (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... and ligated into a pHEN2 vector modified with a triple c-Myc tag108 which was used to transform electrocompetent TG1 cells (Lucigen). The resulting Nb-library consisted of 5×107 independent colonies ...
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bioRxiv - Genetics 2019Quote: ... slowly reduced the temperature to 65°C and then added 2U of Hybridase Thermostable RNase H (Epicenter, Madison, WI: Lucigen H39500) and 1 ul of 10X Digestion buffer (500 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: Total RNAs was treated with RNase R for 30 min at 37°C using 3 U/mg of RNase R (Lucigen, USA). HCC-LM3 and Huh-7 cells treated with actinomycin D (1 µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was then incubated at 37 °C for 1 h with 20 U of RNase R (Lucigen, cat n°RNR07250), to remove all the undesired products (i.e linear RNA or concatemer product) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Crude genomic DNA was generated by incubating yeast isolates at 95°C in 50 μL of 20 mM NaOH for 10 minutes or the MasterPure™ Yeast DNA Purification Kit (Lucigen). PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega) ...
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bioRxiv - Genomics 2019Quote: ... genomic DNA samples (∼2 µg) were end-repaired using the End-it kit from Epicentre Technologies (Madison ...
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bioRxiv - Microbiology 2021Quote: ... the assembled plasmid pool was used to transform 25μl of electrocompetent bacteria (Lucigen #60242-2) on a Bio-Rad Gene-Pulser 2 electroporation system (Bio-Rad # 1652105 ...
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bioRxiv - Microbiology 2020Quote: Random knockout mutants were produced using the EZ::Tn5Tm
2> Tnp TransposomeTm kit (Epicentre) following the manufacturers recommendations ... -
bioRxiv - Cancer Biology 2022Quote: ... The library was transformed into electrocompetent Lucigen Endura™ Escherichia coli (Lucigen; cat. 60242-2) using a Bio-Rad MicroPulser Electroporator (#1652100) ...
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bioRxiv - Molecular Biology 2020Quote: Transposon mutant libraries were constructed using the EZ-Tn5
2>TnP Transposome Kit (Epicentre) according to the manufacturer’s instructions ... -
bioRxiv - Microbiology 2020Quote: ... end-repaired DNA from PoV-01B (1-2 Kb) were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI ...
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bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
2> Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Molecular Biology 2023Quote: ... we utilized the ScriptCap m7G Capping System and ScriptCap 2’-O-methyltransferase kit (Epicentre Biotechnologies) with [α-32P] GTP for our experiments ...
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bioRxiv - Developmental Biology 2019Quote: ... and was then incubated for 30 min at 37 °C with or without 5 U μg −1 of RNase R (Epicentre Bio-technologies). Reverse transcription was performed using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 2 µl water and subjected to electroporation into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI). RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2019Quote: ... One μl of the EZ-Tn5
2> Tnp transposome complex (Epicentre BioTechnologies, Madison, WI, USA) was added to electrocompetent S ... -
bioRxiv - Immunology 2021Quote: ... 40 nanograms of each library were transformed into Endura™ ElectroCompetent Cells (Lucigen cat 60242-2) following the manufacturer’s instructions ...