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Citations for Lucigen :
1 - 50 of 138 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... The products of the Gibson reactions were combined and electroporated into Endura cells (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
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bioRxiv - Genetics 2023Quote: ... the Gibson reaction products were purified by isopropanol precipitation and electroporated into Endura electrocompetent cells (Lucigen, 60242-2). The transformed bacteria were spread onto LB agar plates containing 100 µg/mL ampicillin and incubated overnight at 37℃ ...
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bioRxiv - Systems Biology 2023Quote: ... Eight transformation reactions were conducted with 2 μl of ligation product into each tube of electrocompetent cells (Lucigen, #60242) and plated onto eight 15-cm plates with Carbenicillin selection (50 μg/ml) ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 1x reaction buffer (Lucigen), 2.5 mM MnCl2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Tobacco Acid Pyrophosphatase (Epicentre, discontinued) or recombinant PIR-1 was used to dephosphorylate ppp-RNAs for cloning ppp-RNAs when needed while no such treatment was required for cloning p-RNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... Tobacco acid pyrophosphatase (TAP, Epicentre) was added to convert 5′ PPP or capped RNAs to 5′ P RNAs ...
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bioRxiv - Plant Biology 2020Quote: ... Products were then circularized with CircLigase (Epicentre) according to the manufacturer’s instructions and used as a template for PCR amplification ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl TEX reaction buffer (Lucigen) and 0.5 µl RiboGuard RNase Inhibitor (Lucigen ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x Ampligase® Reaction Buffer (Lucigen), 10 U Ampligase (Lucigen) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were circularized using circligase II (#CL9025K, Epicentre). The major rRNA contaminants were removed using subtractive hybridization with custom biotinylated oligos (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was in vitro transcribed (Epicentre, ASF3507) under the control of promoter T7 for 12 hours at 37°C and DNAse treated with TURBO-DNAse for 20 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The ligated product was electroporated into Electrocompetent cells (Lucigen) and grown in liquid culture overnight at 37 degrees before being extracted by Maxiprep ...
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bioRxiv - Cancer Biology 2023Quote: ... The final product was transformed into electrocompetent cells (Lucigen), incubated overnight in LB broth at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified reactions were electroporated into Endura DUOs (Lucigen) using a MicroPulser with program Ec1 (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the RT product was circularized by CircLigase II (Epicentre). A PCR library was amplified and sequenced using Illumina Nextseq 500 at the Rockefeller University Genomics Center.
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bioRxiv - Genetics 2023Quote: ... The purified product was electroporated into Endura competent cells (Lucigen). We extracted the plasmid pool using the Plasmid Maxi prep kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were incubated with Tobacco Acid Pyrophosphatase (Epicentre) and washed once with wash buffer ...
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bioRxiv - Genomics 2020Quote: ... using a Gibson assembly master mix (New England Biolabs).Gibson assembly products were transformed into electrocompetent cells (E. cloni, Lucigen) and plated on 245mm x 245mm square LB-agar plates to obtain the sufficient number of bacterial colonies at a ∼50× library coverage ...
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bioRxiv - Molecular Biology 2020Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al ...
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bioRxiv - Genomics 2022Quote: ... The product was electrotransformed into Endura™ Competent Cells (Lucigen #60242) and grown on big agar plates ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were then in vitro transcribed with T7 polymerase (Lucigen). Capture SELEX was then carried out as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al. ...
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bioRxiv - Systems Biology 2023Quote: ... the RNA products were treated with Baseline-ZERO DNase I (Epicentre) and then purified again using the RNeasy Mini kit.
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bioRxiv - Cell Biology 2022Quote: Ligation products were transformed into Lucigen Endura Electrocompetent Cells (Lucigen, 60242) using a Gene Pulser II (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2023Quote: ... and gel- purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described36 and libraries constructed using a different reverse indexing primer for each sample.
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bioRxiv - Genetics 2022Quote: ... The ligation products were transferred into Ecloni 10GF’ electrocompetent cells (Lucigen) using Gene Pulser Xcell Electroporation Systems (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... dsRNA was synthesized from in vitro transcription reactions (NxGen, Lucigen). dsRNA corresponding to Caenorhabditis elegans unc-22 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1x reaction buffer (Epicentre cat no. RT80125K and RG90910K). RNA and random decamer primer were first incubated at 85°C for 3 minutes and then incubated on ice for 2 minutes during which remaining reaction components were added ...
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bioRxiv - Genomics 2023Quote: ... including treatment of the ligation reaction with PlasmidSafe DNase (Lucigen)49 ...
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bioRxiv - Genetics 2023Quote: ... 5 Gibson reactions were electroporated into supercompetent cells (Endura, Lucigen) and grown in liquid culture to give a coverage of around 100 million barcodes ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting cDNA products were circularized with CircLigase ssDNA ligase (Lucigen, USA). Illumina sequencing adaptors were introduced by PCR and the final library sequenced at the JHU Genetic Resources Core Facility on a HiSeq2500 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Free nucleic acids were digested using OmniCleave Endonuclease (Lucigen, USA) for 30 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The product was then packaged into phage particles using phage extract (MaxPlax, Epicentre). Plaques were generated on LE392 E ...
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bioRxiv - Molecular Biology 2023Quote: ... The products were then packaged into phage particles using phage extract (MaxPlax, Epicentre) and amplified by lytic growth in LE392 cells (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... coli Cloni® 10G cells (Lucigen, USA; 1ul crude reaction mix). Kanamycin selected clones were screened by colony PCR and verified by sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... A mixture consisting of 1 μl 10x Circligase reaction buffer (Epicentre), 0.5 μl 1 mM ATP ...
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bioRxiv - Microbiology 2020Quote: ... reactions were set up by adding 0.5 uL of RNaseR (Lucigen), 2.5 uL of RNase R buffer and 2 uL of water to the previous set ups (ligated RNA) ...
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bioRxiv - Microbiology 2020Quote: ... NEB Builder reaction mixtures were transformed into E.coli EPI300 cells (Lucigen) and successful integration of the S protein cDNA was confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation reaction was then treated with PlasmidSafe (Epicentre, Cat#: E3101K) to digest any residual linearized DNA ...
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bioRxiv - Immunology 2023Quote: ... The ligation reaction was purified and transformed into TG1 bacteria (Lucigen) by electroporation (1.8 kV ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixtures were further transformed into TG1 electrocompetent cells (Lucigen). The cells were rescued with 1 ml of recovery medium for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Purified RNAs were then treated with TAP (Tobacco Acid Pyrophosphatase, Epicentre) to convert 5′ ends of RNA to monophosphate ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation product was treated with Plasmid-safe nuclease (10,000 units/ml, Epicentre, E3101K) and transformed into SURE2 cells ...
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bioRxiv - Genetics 2022Quote: ... The ligated product was then electroporated into Enduratm electro-competent cells (Lucigen #60242-1) in 2 separate reactions ...
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bioRxiv - Evolutionary Biology 2021Quote: Then the PCR products were ligated into vector pSmart LC Kn (Lucigen, cat.# 40821) and electroporated into E ...
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bioRxiv - Genomics 2021Quote: ... MDA reactions were run under clean conditions using the RepliPhi kit (Epicentre) in a 15 ul reaction volume in 96-well plates or with the Repli-g Single Cell kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation reaction above was then treated with PlasmidSafe (Epicentre, Cat#: E3101K) to digest any residual linearized DNA ...
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bioRxiv - Immunology 2022Quote: ... The Gibson assembly reaction was electroporated using Endura(tm) ElectroCompetent Cells (Lucigen), plated on LB + Ampicillin plates at an estimated 150,000 colonies per replicate library to limit library complexity ...