Labshake search
Citations for Lucigen :
1 - 50 of 699 citations for Glutathione Colorimetric Detection Kit 200 High Cuvettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... The AmpliScribe™ T7 High Yield Transcription Kit was purchased from Epicentre and the HiScribe T7 In Vitro Transcription Kit was obtained from New England Biolabs ...
-
bioRxiv - Developmental Biology 2024Quote: ... The dsRNA was synthesized using the AmpliScribe T7 High Yield Transcription Kit (Lucigen, USA). Primer sequences for the PCR amplification are provided in Supplementary Table S3.
-
bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
-
bioRxiv - Microbiology 2022Quote: ... high molecular weight DNA was isolated using the MasterPure DNA Purification kit (Epicentre, no. MC85200). Sequencing was performed using the PacBio Sequel (Pacific Biosciences ...
-
bioRxiv - Microbiology 2019Quote: ... 200 ng of template DNA were transcribed using the AmpliScirbe T7 Flash Transcription Kit (Epicentre) following the manufacturer’s recommendations ...
-
bioRxiv - Genomics 2020Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
-
bioRxiv - Plant Biology 2021Quote: ... complementary RNA (cRNA) was prepared with the AmpliCap-Max™ T7 High Yield Message Maker Kit (Epicentre Biotechnologies). Oocyte preparation and cRNA injection were performed as previously described [94] ...
-
bioRxiv - Genetics 2022Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
-
bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
-
bioRxiv - Microbiology 2023Quote: High molecular weight genomic DNA was extracted using the MasterPureTM Gram Positive DNA Purification Kit (Epicentre, Lucigen, USA). Cells from two plates were re-suspend in 1.5mL 1X PBS and harvested by centrifugation ...
-
bioRxiv - Microbiology 2019Quote: ... Total genomic DNA was extracted from 200 μl of blood using a MasterPure DNA purification kit (Epicentre® Biotechnologies ...
-
bioRxiv - Microbiology 2021Quote: Phage DNA extractions were performed from high titer suspensions using the MasterPureTM Complete DNA and RNA Purification Kit (Epicentre), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2022Quote: High molecular weight DNA was extracted using either the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), or by standard phenol/chloroform extraction ...
-
bioRxiv - Microbiology 2023Quote: High molecular weight DNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. Seven days old-mycelia of P ...
-
bioRxiv - Neuroscience 2021Quote: ... 6µl 500X CopyControl Fosmid Autoinduction Solution was also added to induce the fosmid to high copy according to the CopyControl Fosmid Library Production Kits (EPICENTRE). The fosmid was then prepared using the standard Qiagen miniprep kit.
-
bioRxiv - Genetics 2020Quote: ... and used as a template for in-vitro transcription of gRNA by AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies).
-
bioRxiv - Molecular Biology 2021Quote: ... or XhoI- (for plasmids with the prefix “pUC57-”) digested plasmids or PCR products using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), followed by capping with ScriptCap m7G Capping System (Cell Script) ...
-
bioRxiv - Biochemistry 2024Quote: The mRNA coding for the catalytic mutant AGOs used in the equilibrium binding assay was transcribed in vitro using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), employing the corresponding pBYL-3×FLAG-SUMO-catalytic mutant AGO plasmids linearized with Not I as templates ...
-
bioRxiv - Genetics 2020Quote: ... Purified PCR products were then used as templates for in vitro transcription per AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies) specifications to obtain dsRNAs.
-
bioRxiv - Genomics 2019Quote: DNA for ChIP and other high throughput sequencing approaches was processed as follows: End repair was performed using an Epicenter End-It kit (Lucigen ER0720), according to manufacturer’s instructions and column purified in a Zymo Minelute column (Zymo D4013) ...
-
bioRxiv - Neuroscience 2024Quote: ... the pGEMHE plasmids carrying different KCR1 variants were linearized by NheI digestion and used for in vitro transcription of complementary RNA (cRNA) using the AmpliCap-Max T7 high-yield message maker kit (Epicentre Biotechnologies). For all the KCR1 expression variants ...
-
bioRxiv - Genetics 2020Quote: Ribosomal RNA-depleted RNA-seq libraries were prepared from 200-500 ng of total RNA using the low input ScriptSeq Complete Gold Kit (Epicentre). Libraries were sequenced on an Illumina NextSeq 500 with single-end 75 bp reads.
-
bioRxiv - Immunology 2023Quote: ... 100-200 μL QuickExtract solution (Epicentre) was directly added to cells and incubated at 65 °C for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... two ml of a culture grown in R2B were used for extraction of high molecular weight (HMW) DNA using the MasterPureTM DNA Purification Kit (Epicentre, Madison, WI, USA), using the kit’s protocol for cell samples ...
-
bioRxiv - Neuroscience 2020Quote: ... or the AmpliCap High Yield Message Maker (Epicentre) kits.
-
bioRxiv - Molecular Biology 2022Quote: ... and 1:200 diluted endonuclease (Lucigen: OC7850K) was immediately added to the pulverized tissue at a constant ratio of 15μL/mg of tissue ...
-
bioRxiv - Genomics 2021Quote: ... 200 U of NxGen RNase Inhibitor (Lucigen), and protease inhibitor cocktail (Roche ...
-
bioRxiv - Developmental Biology 2020Quote: We used 200-250 primordial follicles and 100-150 primary follicles for cDNA synthesis using the Message Booster cDNA synthesis kit (Lucigen, Palo Alto, CA). Direct cDNA and subsequent cRNA syntheses were performed by following the manufacturer’s instruction ...
-
bioRxiv - Immunology 2023Quote: ... and 200 U NxGen RNase inhibitor (Lucigen, #30281). Extracts were incubated on ice for 5 min prior to centrifugation at 17,000 g for 5 min at 4 °C ...
-
bioRxiv - Microbiology 2024Quote: ... Between 200- 500 ng of enriched mRNA was used for cDNA production using the ScriptSeq®v2RNA-Seq Library Preparation Kit (Epicentre, Madison, WI, USA), FailSafe®PCR Enzyme Mix (Epicentre ...
-
bioRxiv - Genomics 2020Quote: ... washed with PBS and resuspended in 200 μL of Quick Extract (Lucigen) lysis buffer and cells were lysed according to the manufacture’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Comparable results to commercial RNA extraction kits have been obtained using a 5-min direct detection preparation method of nasopharyngeal samples following 1:1 dilution with the Quick Extract DNA extraction Solution (Lucigen) (6) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and lysed in containing 200 μl of QuickExtract™ DNA Extraction Solution (Lucigen Cat. # QE09050) per well of 48-well plates ...
-
bioRxiv - Neuroscience 2022Quote: cDNA was made from 200 ng of labellar RNA as template from using EpiScript (Lucigen). Two biological replicates were prepared per species ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 400ng of total RNA was used to produce cDNA using MMLV High Performance Reverse Transcriptase (Lucigen RT80125K). TaqMan qPCR was performed using PrimeTime® Gene Expression Master Mix IDT ...
-
bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified ligation product was transformed into 200 µL of electrocompetent TG1 cells (Lucigen, 60000-PQ763-F) followed by addition of 6 mL of recovery medium (Lucigen ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were lysed with 1 ml mammalian lysis buffer containing 200 µl 5x Mammalian Polysome Buffer (Epicentre, RPHMR12126), 100 µl 10% Triton X-100 ...
-
An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Lysate corresponding to 30 μg RNA was diluted to 200 μl in polysome buffer and digested with 1.5 μl RNaseI (Epicentre) for 45 min at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... The puck was immersed in 200 µL of hybridization buffer (6X SSC, 2 unit / µL Lucigen NxGen RNAse inhibitor) for 30 minutes at 37°C for the binding of RNA to the oligos ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysate corresponding to 15 µg RNA was diluted to 200 µl in polysome buffer and digested with 0.75 µl RNaseI (Epicentre) for 45 min at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
-
bioRxiv - Genomics 2020Quote: ... Multiple PCR reactions for each sample were carried out with 200 pg of annealed DNA using the XpYpE2 and teltail primers (Supplementary Table 3) and FailSafe PCR reagents (Epicentre). PCR conditions were as follows ...
-
bioRxiv - Molecular Biology 2022Quote: ... 30 μg of lysate was diluted to 200 μL in polysome buffer (lysis buffer without Triton X-100) and 1.5 μL RNase I was added (Epicentre #N6901K). RNase digestion was carried out at 200 rpm at 37°C for 45 minutes ...
-
bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor ...
-
bioRxiv - Molecular Biology 2022Quote: Approximately 1e+06 cells were harvested two days post nucleofection and incubated in 200 μL of QuickExtract DNA Extraction Solution (Lucigen) at 65ºC for 15 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmid Safe DNase kit (EpiCentre) was used to degrade any unspecific recombination products for 30 min at 37 °C ...
-
bioRxiv - Genomics 2019Quote: ... ARTseqTM kit from Epicentre (#RPHMR12126); RNaqueous kit from Ambion (#AM1912) ...
-
Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
-
bioRxiv - Molecular Biology 2020Quote: ... The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.