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Citations for Lucigen :
351 - 400 of 604 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed and genomic DNA was extracted using QuickExtract DNA Extraction Buffer (Lucigen) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and RNA extracted with MasterPure™ Yeast RNA Purification Kit (Epicentre). Samples were vacuum dried and sent to Genewiz (South Plainfield ...
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cell lines using QuickExtract DNA Extraction Solution (Epicentre, #QE09050). Specifically ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng of library was added to 25 µL of Endura electrocompetent cells (Lucigen). Cells were electroporated using the MicroPulser (BioRad ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were transformed into E.cloni ® 10G Chemically Competent Cells (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... genomic DNA was isolated from the transfected cells using DNA QuickExtract™ Buffer (Lucigen) in adherence to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using the MasterPure RNA Purification Kit from Epicentre following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... expanded by 16 electroporations (8 for each half library) into Endura electrocompetent cells (Lucigen), and plated on sixteen 24.5 cm bioassay plates (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from cells 72 hours post-transfection with QuickExtract (Lucigen #QE0905) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage of around 200x.
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bioRxiv - Cell Biology 2023Quote: ... Electroporation was performed according to manufacturer’s instructions into Endura ElectroCompetent Cells (Lucigen, 60242-2). Dilution of transformed cells and estimation of the number of colony forming units allowed for calculation of plasmid library coverage ...
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bioRxiv - Biochemistry 2023Quote: ... the library was electroporated into Escherichia coli cells (E. cloni 10G Elite; Lucigen, USA), yielding ≈ 107 colonies after overnight incubation on agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were lysed and solubilised with 2000 U of Ready-Lyse Lysozyme (Epicentre) and 0.5% SDS (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from transfected cells using the QuickExtractTM DNA Extraction Solution (Lucigen) and mutations were detected by the SURVEYOR assay (Integrated DNA technologies) ...
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bioRxiv - Microbiology 2023Quote: ... 300 μl of yeast cell lysis solution (from Epicentre MasterPure Yeast DNA Purification kit), 0.3 μl of 31,500 U/μl ReadyLyse Lysozyme solution (Epicentre ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... the assembled library plasmid pool was electroporated into Endura cells (Lucigen, Cat #60242-2) at 50-100 ng/µl ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were collected after 3 days and lysed with QuickExtract DNA Extraction Solution (Lucigen): endogenous loci were PCR amplified with HOT FIREPol MultiPlex Mix (Solis BioDyne) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA of 3T3 cells was isolated with QuickExtractTM DNA Extraction Solution 1.0 (QE09050, Lucigen). Presence of the homozygous deletion was confirmed by PCR using specific primers ...
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bioRxiv - Genomics 2023Quote: ... 1.5 µL BP reaction was electroporated into 25 µL Endura Electrocompetent cells (Lucigen, 60242) with the following conditions ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) containing 50 U/μL Ready-Lyse Lysozyme (Lucigen #R1804M) and protease inhibitors ...
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bioRxiv - Genetics 2022Quote: Transposon mutagenesis was performed using the EZ-Tn5
1> transposon kit (Epicentre) and EK54/MA56/LP372 Tn5 transposase ... -
bioRxiv - Microbiology 2021Quote: ... the remainder of samples were lysed by adding 1 ul of ReadyLyse (EpiCentre) and incubating at room temperature for 12 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and subjected to end-repair with T4 PNK (Lucigen, 37C for 1 hr). End-repaired RNA was cleaned up with the RNA Clean and Concentrator kit (Zymo) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli genomic DNA in 15 μL of 1× Ampligase DNA Ligase Buffer (Epicentre) containing 250 ng of unshared E ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Genomics 2019Quote: ... One microliter of the reaction mix was used to transform ultra-competent EPI300 cells (Epicentre). Telomere-positive clones were identified by colony blotting.
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bioRxiv - Immunology 2019Quote: ... Genomic DNA was extracted from candidate RAB1B KO cells using QuickExtract DNA Extraction Solution (Lucigen), as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from pelleted cells using a MasterPure Yeast RNA extraction kit (Lucigen, MPY03100), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The genomic DNA from individual cell clones was extracted using QuickExtract DNA Extraction Solution (Lucigen) and screened by PCR for the deletion amplicon using the DNA primers listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... was resuspended in TE and introduced into BL21(DE3) Hi-Control chemically competent cells (Lucigen), which were selected on LB plates supplemented with kanamycin.
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bioRxiv - Genomics 2022Quote: ... the clean-up DNAs underwent the protocol SYGNIS TruePrimeTM WGA & Single Cell WGA Kits (Lucigen), instead of the REPLI-g single cell kit ...
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bioRxiv - Genomics 2020Quote: ... ethanol precipitated and transformed into 200l of electrocompetent E.coli (Lucigen Endura™ ElectroCompetent Cells, Lucigen). 4 x 250ml LB preps (supplemented with 100 μg mL-1 carbenicillin ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from at least 104 harvested cells using the QuickExtract protocol (Lucigen). The target locus was then amplified by PCR with forward and reverse primers where at least one primer annealed outside the integration site (Supplementary Table 1) ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 104 to 105 T cells was was extracted using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using primers F2(RB198)* and R2(RB199)* primers (Supplemental Table 1) ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and positional insertion of mGFP/mScarlet-I cassette ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). Primers specific to the blasticidin resistance cassette and IRAK1/IRAK4 gene loci were used to verify the insertion (see Table S5 and S6 for IRAK4 and IRAK1 respectively) ...
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Intra-species transcriptional profiling reveals key regulators of Candida albicans pathogenic traitsbioRxiv - Genomics 2021Quote: ... RNA was harvested from cells using a Masterpure Yeast RNA Purification kit (Epicentre, Madison, WI) and treated with DNaseI (Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from each of the four cell pools with 100 µL QuickExtract (Lucigen) per 1 million cells following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted from cell pellets using the MasterPure yeast DNA purification kit (Epicentre). Melanin was removed from genomic DNA using the OneStepTM PCR Inhibitor Removal kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Genomics 2023Quote: ... Resulting colonies were picked and a portion of the cells lysed by QuickExtract (Lucigen, QE9050) and used to genotype by PCR with primers on either side of the insertion site (in most cases with one primer outside the homology arms ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and correct insertion of mGFP/mScarlet-i cassette ...
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bioRxiv - Molecular Biology 2023Quote: ATAC-see in 293T cells was done with the EZ-Tn5™ Transposase (Lucigen TNP9211), following the published protocol36 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
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bioRxiv - Microbiology 2023Quote: ... These recombinant plasmids were then introduced into BAC-Optimized Replicator v2.0 electrocompetent cells (60210, Lucigen) via electroporation at 2500V ...
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bioRxiv - Microbiology 2024Quote: ... pneumoniae ECL8 electrocompetent cells were transformed with 0.2 µL of EZ-Tn5™ transposon (Epicentre) at 1.4 kV and recovered in brain heart infusion (BHI ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10G isa highly competent DH10B derivative [54] originally obtained from Lucigen (60107-1). E ...