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Citations for Lucigen :
451 - 500 of 1209 citations for DNA Damage 8 OHdG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and positional insertion of mGFP/mScarlet-I cassette ...
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bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). Primers specific to the blasticidin resistance cassette and IRAK1/IRAK4 gene loci were used to verify the insertion (see Table S5 and S6 for IRAK4 and IRAK1 respectively) ...
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bioRxiv - Immunology 2020Quote: ... DNA was isolated from the clones using the QuickExtract DNA Extraction Solution (Lucigen, Middleton, USA). Upon amplification and sequencing (Eurofins Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen, Middelton, WI, USA). 50 ng of genomic DNA were used to amplify the region that spans the cutting site of each gRNA using KAPA2G Fast ReadyMix (Kapa Biosystem ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and correct insertion of mGFP/mScarlet-i cassette ...
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bioRxiv - Microbiology 2020Quote: ... the RNA fragments were poly(A)-tailed and 5’PPP structures were removed with RNA 5’ Polyphosphatase (Epicentre). The RNA sequencing adapter with the barcodes were ligated to the 5’-monophosphate of the fragments ...
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bioRxiv - Neuroscience 2020Quote: ... coli 5-alpha Chemically Competent cells (Lucigen), performing a thermal shock for 45 seconds at 42°C followed by 2 minutes on ice ...
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bioRxiv - Biochemistry 2019Quote: ... transcripts were treated with 5’-polyphosphatase (Epicentre) and Xrn1 (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... transcripts were treated with 5’-polyphosphatase (Epicentre) and Xrn1 (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and Terminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Molecular Biology 2023Quote: Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen) was used to enrich the 5′ ends of the transcripts ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA from infected barley leaves at 6 days post-inoculation (dpi) was isolated by using the MasterPure™ Complete DNA&RNA purification Kit (Epicentre®, Illumina®) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Genetics 2021Quote: ... Total genomic DNA of hybrids was extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen). DNA concentration was measured using the Qubit dsDNA HS assay (ThermoFischer ...
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bioRxiv - Biophysics 2020Quote: Genomic DNA was isolated from candidate cell lines using the QuickExtract DNA Extraction Solution (Lucigen, #QE09050) essentially as described ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified linearized DNA was ligated to the JEBPN-DNA linker (Table S6) using Circligase II (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA from 2.5E5 cells was extracted with 250 µl of QuickExtract DNA extraction solution (Lucigen) per manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2020Quote: ... and genomic DNA was extracted using 50 μL of Quick Extract DNA Extract Solution (Lucigen, QE09050) per well according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
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bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre Cat. # QE09050) to the cells ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre, Cat # QE09050) to the cells ...
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bioRxiv - Genetics 2024Quote: Genomic DNA from ear punches was isolated using Quick Extract DNA Extraction Solution (Lucigen., Cat# QE09050). All genotyping PCRs were carried out using MyTaq™ Red Mix (Bioline ...
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bioRxiv - Molecular Biology 2020Quote: Quick extract DNA extraction solution (Lucigen) was tested in accordance with the manufacturer’s suggested buffer to sample ratio ...
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bioRxiv - Microbiology 2021Quote: ... and DNA extraction with QuickExtract (EpiCentre) was performed ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was harvested with QuickExtract (Lucigen) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2023Quote: ... QuickExtract DNA Extraction solution from Lucigen; EDTA-free Complete Protease Cocktail from Roche ...
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bioRxiv - Genetics 2024Quote: ... DNA Quick Extract (Lucigen # 76081-766) was used to isolate DNA ...
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bioRxiv - Microbiology 2024Quote: DNA was prepared using MasterPure (Epicentre) from 500 μL samples from overnight cultures of constructed clones or evolving populations grown at 30 °C or 37 °C in LB ...
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bioRxiv - Genomics 2019Quote: ... and the 5’P of capped molecules was exposed by treatment with 5 units of Tobacco Acid Pyrophosphatase (Epicentre). RNA samples were ligated with the TIF-seq DNA/ RNA 5oligo cap using T4 RNA ligase 1 (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA templates were prepared by lysing cells in QuickExtract DNA extraction solution (Lucigen, Cat No. QE0905T). Target regions were amplified by using specific PCR primers (ETV2_F ...
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bioRxiv - Cell Biology 2022Quote: ... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
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bioRxiv - Cell Biology 2022Quote: ... the individual clones were expanded and the DNA was isolated using the QuickExtract DNA Extraction Solution (Lucigen). A successful homozygous genomic modification was confirmed by PCR (data not shown ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... linear contaminant DNAs within the crude circular DNA are digested with Plasmid-Safe ATP-dependent DNase (Lucigen) at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... Genomic DNA was extracted 3 days post-transfection using QuickExtract DNA Extraction Solution 1.0 (Lucigen Corporation QE09050). To test the cutting efficiency ...
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bioRxiv - Microbiology 2022Quote: ... The pilT gene and flanking sequence from the FA1090 chromosome was amplified by PCR with primers PilT-F 5’-CATTGAGGTCGGCAAGCAGC-3’ and PilT-R 5’-GCATCTTTACCCAGCGCGAAAT-3’ and cloned into pSMART HC Kan (Lucigen). The cloning mix was transformed into TOP10 E ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then poly(A)-tailed using poly(A) polymerase and the 5’-PPP was removed using 5’ polyphosphatase (Epicentre). RNA adaptors were then ligated and synthesis of first strand cDNA was performed using M-MLV reverse transcriptase and oligo(dT ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 µl Hybridase (Lucigen, H39500, 5 units/µl) and 2.5 µl water were added to the mix ...
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bioRxiv - Microbiology 2021Quote: ... samples were treated with RNA 5‘ polyphosphatase (Lucigen). The 5‘ Illumina TruSeq sequencing adapter carrying sequence tag TAATGCGC was ligated to newly formed 5‘-P RNA ends ...
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bioRxiv - Cell Biology 2021Quote: ... cloni 5-alpha (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Molecular Biology 2021Quote: ... and T erminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... we treated these samples with 5’ polyphosphatase (Lucigen) (20 U per 5 μg of RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... RNase I/Hybridase (Lucigen, 55C for 5 minutes) and the Oligo Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from modified cell lines using QuickExtract DNA extraction buffer (Epicentre Technologies, Madison, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the genomic DNA derived from transfected single cell colonies was extracted with QuickExtractTM DNA Extraction Solution (QE09050, Epicentre) for Sanger sequencing and the genomic DNA with biallelic editing was further subjected to whole-genome sequencing ...