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Citations for Lucigen :
701 - 750 of 1243 citations for DNA Damage 8 OHdG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... genomic DNA from bulk cells of each transfected well was extracted using Quick Extract buffer (Lucigen #QE0905T). following the protocol published by the manufacturer (https://www.lucigen.com/docs/manuals/MA150E-QuickExtract-DNA-Solution.pdf) ...
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bioRxiv - Genetics 2019Quote: ... Another 10% of the cell culture was used for DNA extraction using 15 uL of QuickExtract (Lucigen). For the timecourse optimization experiment ...
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bioRxiv - Developmental Biology 2022Quote: ... yolk sacs were dissected and a small fraction was placed in QuickExtract DNA Extraction Solution (Lucigen, QE09050) and processed according to manufacturer’s protocols.
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bioRxiv - Developmental Biology 2022Quote: Tissue samples (ear and tail clips) from resulting animals were lysed using QuickExtract DNA Extraction Solution (Epicentre) followed by PCR at the specific genomic location ...
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bioRxiv - Neuroscience 2023Quote: ... sorter was used to collect rods and cones into 20 µL of QuickExtract DNA Extraction Solution (Lucigen). The numbers of sorted cells ranged from 200- to 750 cones and 2,000- to 6,500 rods for human retinas (Figure 2G) ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal cell lines were isolated by single-cell sorting and characterized by genomic DNA extraction (Lucigen QuickExtract), PCR amplification of edited loci using Q5 polymerase (NEW ENGLAND BIOLABS ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA was extracted from individual female adult flies from stocks of interest using QuickExtract solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and three libraries for each sex were generated with the NEBNext Ultra RNA library prep kit combined with rRNA depletion via the Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies). Samples were sequenced on an Illumina HiSeq2500 platform at the Vienna BioCenter Core Facilities (VBCF ...
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bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Ribo Zero kit and samples were converted into Illumina sequencing libraries using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina). Libraries were sequenced using Illumina HiSeq (2 × 150 bp reads) ...
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bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... ruptured cells were treated with 1 U μl−1 OmniCleave endonuclease (Lucigen) for 15 min at 37°C to release the DNA-bound protein pool ...
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bioRxiv - Immunology 2022Quote: ... from Lucigen (60810-1), were used in this study ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
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bioRxiv - Bioengineering 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed in 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) using a SimpliAmp Thermal Cycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed in 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) using a SimpliAmp Thermal Cycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 1000 ng of total RNA was used to remove the DNA contamination using Baseline-ZERO™ DNase (Epicentre) following the manufacturer’s instructions followed by purification using the RNA Clean & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligated DNA was packaged into virions using the MaxPlax λ packaging extract (Lucigen #MP5120, Middleton, WI, USA), and the virions were sequenced to determine input barcode frequency.
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bioRxiv - Molecular Biology 2020Quote: ... DNA template was incubated at 37°C overnight in a transcription mix containing: transcription buffer 1X (Epicentre Biotechnologies), 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Immunology 2021Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Clones were identified ∼2 weeks after sorting and screened by genomic DNA extraction with Quickextract (Lucigen cat.#QE09050) and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712) ...
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bioRxiv - Developmental Biology 2020Quote: ... complementary tandem repeats were generated through rolling-circle amplification (RCA) with NxGen® phi29 DNA Polymerase (Lucigen, 30221). The single-stranded amplicons were detected with a Cy5-labeled oligonucleotide (Cy5-DP5 ...
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bioRxiv - Genomics 2023Quote: ... The MDA reactions were prepared using RepliPHI Phi29 DNA polymerase and Reagent set (Epicentre, Illumina, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Microbiology 2023Quote: ... Around 10 000 colonies were harvested and pooled prior to DNA extraction using Ready-Lyse Lysozyme (Epicentre Lucigen) and DNAzol (ThermoFischer) ...
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bioRxiv - Biophysics 2023Quote: ... DNA templates for use in in vitro transcription reactions were amplified with EconoTaq PLUS 2x Master Mix (Lucigen) using primers UNIV-pUC19_E105 and miR_tail_3buffer_REV (DMS ...
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bioRxiv - Genetics 2023Quote: ... 15-20 μg of genomic DNA (gDNA) was digested with ∼95 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Molecular Biology 2024Quote: ... C-circle and G-circles assays were performed as before except that NxGen phi29 DNA Polymerase (Lucigen Corp.) were used for rolling circle amplifications [25].
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bioRxiv - Biochemistry 2024Quote: ... DNA templates for use in in vitro transcription reactions were amplified with EconoTaq PLUS 2x Master Mix (Lucigen) using primers UNIV-pUC19_E105 and HH-pre-let7c-AMP-R (Table S2).
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bioRxiv - Genomics 2024Quote: ... and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen), Lambda exonuclease (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and the DNA adaptor was added for cDNA adaptor ligation using CircLigase™ II ssDNA Ligase (CL9021K, Lucigen) as described by the product instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... we used the MasterPure RNA purification kit from Epicentre instead of phenol/chloroform extraction to purify RNA after the dephosphorylation and ligation steps.
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bioRxiv - Developmental Biology 2021Quote: Founder mice were genotyped using FailSafe PCR Kit (EpiCentre) and run on a 12% polyacrylamide gel for heteroduplex assay ...
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bioRxiv - Plant Biology 2020Quote: ... Ribo-Zero kit (Epicentre, an Illumina company, Madison, WI) was used to remove rRNA from the libraries ...
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bioRxiv - Microbiology 2021Quote: The ScriptSeq complete kit (Epicentre, Illumina, Madison, WI USA), a combined kit for the ribosomal (rRNA ...
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bioRxiv - Molecular Biology 2023Quote: Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina) was used to remove ribosomal RNAs and to enrich mRNAs ...
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bioRxiv - Biochemistry 2023Quote: ... AmpliScribe™ T7-Flash™ Transcription Kit (ASF3507, Lucigen) was used according to the manufacturer protocol with 4 μL PCR products per 20 μL reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... C-circle and G-circles assays were performed as before except that NxGen® phi29 DNA Polymerase (Lucigen Corp.) were used for rolling circle amplifications [25].
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bioRxiv - Biochemistry 2019Quote: The DNA constructs of Loa22 were individually transformed into expression host cell E.coli ClearColiTMBL21 (DE3) pLys (Lucigen, Middleton, WI). Loa22 and its variants were grown at 37°C in the medium with 50 μg/mL ampicillin to an OD600 of approximately 0.6 ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... waltii isolates as well as the generated hybrids were extracted using a modified MasterPure Yeast DNA purification protocol (Lucigen).
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bioRxiv - Microbiology 2020Quote: ... HBV cccDNA was quantified from total DNA following digestion for 45 min at 37°C with T5 exonuclease (Epicentre) to remove rcDNA followed by 30 min heat inactivation ...
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bioRxiv - Cancer Biology 2021Quote: ... These primers were used to amplify cell pools after DNA extraction using Quick Extract (Lucigen, Wisconsin, USA, Cat #QE09050). Amplified reads were sequenced on either an Illumina MiSeq or Illumina HiSeq 2500.
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bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1.2ug of ligated pUSEPR plasmid DNA per sub-pool was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37°C ...
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bioRxiv - Genomics 2022Quote: ... A total of 1.2 µg of ligated pUSEBR-CRISPR Library plasmid DNA was then electroporated into Endura electrocompetent cells (Lucigen). Competent cells were recovered for 1 h at 37ºC ...
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bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.
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bioRxiv - Cancer Biology 2020Quote: ... HBV plasmid (positive control) and HBV DNA from inoculum was digested with plasmid-safe DNase (Epicentre, E3101K, Madison, WI). According to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified genomic DNA from each individual was used to prepare paired-end libraries (Lucigen Shotgun library preparation, PCR free) and sequenced on an Illumina HiSeq X PE 150 bp platform to 10-20X coverage at the Centre d’ Expertise et de Services ...
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bioRxiv - Bioengineering 2023Quote: ... Supernatant were carefully discarded and the isolated hair cells were lysed by 5μl QuickExtract™ DNA Extraction Solution (Lucigen) and incubated in 65°C for 6 min then 98°C 3 min ...