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Citations for Lucigen :
51 - 100 of 839 citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the RNA fragments were poly(A)-tailed and 5’PPP structures were removed with RNA 5’ Polyphosphatase (Epicentre). The RNA sequencing adapter with the barcodes were ligated to the 5’-monophosphate of the fragments ...
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bioRxiv - Neuroscience 2020Quote: ... coli 5-alpha Chemically Competent cells (Lucigen), performing a thermal shock for 45 seconds at 42°C followed by 2 minutes on ice ...
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bioRxiv - Biochemistry 2019Quote: ... transcripts were treated with 5’-polyphosphatase (Epicentre) and Xrn1 (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... transcripts were treated with 5’-polyphosphatase (Epicentre) and Xrn1 (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and Terminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Molecular Biology 2023Quote: Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen) was used to enrich the 5′ ends of the transcripts ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ml of 37 °C Recovery Medium (Lucigen, 80026-1) was added and bacteria were grown in round-bottom tubes for 1 h at 37 °C while shaking at 180 r.p.m ...
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bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
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bioRxiv - Genomics 2019Quote: ... and the 5’P of capped molecules was exposed by treatment with 5 units of Tobacco Acid Pyrophosphatase (Epicentre). RNA samples were ligated with the TIF-seq DNA/ RNA 5oligo cap using T4 RNA ligase 1 (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... The pilT gene and flanking sequence from the FA1090 chromosome was amplified by PCR with primers PilT-F 5’-CATTGAGGTCGGCAAGCAGC-3’ and PilT-R 5’-GCATCTTTACCCAGCGCGAAAT-3’ and cloned into pSMART HC Kan (Lucigen). The cloning mix was transformed into TOP10 E ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then poly(A)-tailed using poly(A) polymerase and the 5’-PPP was removed using 5’ polyphosphatase (Epicentre). RNA adaptors were then ligated and synthesis of first strand cDNA was performed using M-MLV reverse transcriptase and oligo(dT ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 µl Hybridase (Lucigen, H39500, 5 units/µl) and 2.5 µl water were added to the mix ...
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bioRxiv - Microbiology 2021Quote: ... samples were treated with RNA 5‘ polyphosphatase (Lucigen). The 5‘ Illumina TruSeq sequencing adapter carrying sequence tag TAATGCGC was ligated to newly formed 5‘-P RNA ends ...
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bioRxiv - Cell Biology 2021Quote: ... cloni 5-alpha (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Molecular Biology 2021Quote: ... and T erminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... we treated these samples with 5’ polyphosphatase (Lucigen) (20 U per 5 μg of RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... RNase I/Hybridase (Lucigen, 55C for 5 minutes) and the Oligo Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Biochemistry 2020Quote: ... Mutant proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600 ∼0.4 ...
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bioRxiv - Neuroscience 2020Quote: ... using Inpp5e WT forward primer 5’-GAGAAGCTGATAGATGGCTAGG and Inpp5e WT reverse primer 5’-AACCAGAAGACCTCATCAAACC and EconoTaq PCR according to manufactures specifications (Lucigen corporation, WI) (Fig ...
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bioRxiv - Systems Biology 2023Quote: ... and recovered for one hour at 37°C in Recovery Media (Lucigen). Aliquots from the transformations were used to inoculate overnight cultures of LB containing 25 μg/mL of Zeocin (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... 3-5 μl Hybridase™ Thermostable RNase H (Lucigen) and 7 μl 10x RNase H buffer preheated to 45°C was added ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted from one plate using QuickExtract DNA extraction solution (Epicentre) to perform genotyping by PCR using primer pairs specifically recognizing the mutated sequences introduced by the ssODN and a genomic sequence adjacent to the region of integration ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from 96-well plates of cells using QuickExtract (Epicentre) by incubation at 65°C for 6 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Wild-type proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600∼0.4 ...
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bioRxiv - Genomics 2019Quote: ... The DNA was inserted into the vector pIndigoBAC-5 (Epicentre, Madison ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were treated with Terminator 5′ phosphate-dependent exonuclease (Epicentre) to remove RNAs with 5′ monophosphate (5′ P ...
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bioRxiv - Molecular Biology 2021Quote: ... Each aliquot was either enzymatically treated with 5’ polyphosphatase (Lucigen) (20 U per 5 μg of RNA ...
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bioRxiv - Genetics 2019Quote: ... 5 μL FailSafe 2× PCR premix G (EpiCentre, Madison, Wisconsin), approximately 25 ng genomic DNA template ...
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bioRxiv - Genetics 2023Quote: ... 5 Gibson reactions were electroporated into supercompetent cells (Endura, Lucigen) and grown in liquid culture to give a coverage of around 100 million barcodes ...
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bioRxiv - Biochemistry 2023Quote: ... coli (C41) cells for protein expression were purchased from Lucigen Corporation (Wisconsin) ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of Hybridase Thermostable RNase H at 45°C (Lucigen (now Biosearch Technologies), Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 2min at 72 C) using the EconoTaq Plus protocol (Lucigen, Middleton, WI, United States). The PCR products were then digested ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were sequentially treated with Terminator 5‘-Phosphate-Dependent Exonuclease (Lucigen), Quick CIP (calf-intestine alkaline phosphatase ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cloni® 10G (MP21-5) was obtained from Lucigen (WI, USA). All strains used and constructed in this study are shown in Tab ...
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bioRxiv - Genomics 2023Quote: ... We added Terminator™ 5-Phosphate-Dependent Exonuclease (Lucigen, Wisconsin, USA) into one part for the +TEX sample following the user’s manual and skipped this step for the other half for -TEX sample ...
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bioRxiv - Plant Biology 2024Quote: ... followed by incubation with Terminator 5′-Phosphate-Dependent Exonuclease (TEX) (Lucigen) to remove all residual RNAs containing 5’ monophosphate ...
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by precipitation with MCP solution (Lucigen, WI, USA) and the supernatant was collected after centrifugation at 17,000 x g for 10 min at 40C ...
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bioRxiv - Genomics 2020Quote: To test the Terminator™ 5’-phosphate-dependent exonuclease (Lucigen, Cat. # TER5120), 100 ng of total RNA in 2 μL was combined with 18 μL TEX mix ...
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bioRxiv - Genomics 2021Quote: Monophosphorylated RNAs were selectively degraded by Terminator 5’-phosphate-dependent exonuclease (Lucigen). Subsequent 5’ dephosphorylation by CIP (NEB ...
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bioRxiv - Genetics 2021Quote: ... RNA samples for small RNA-seq were treated with 5’polyphosphatase (Lucigen) and prepared using the SMARTer Apollo system with modifications for small RNA library preparation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sample was treated with 20 units of 5’ RNA polyphosphatase (Lucigen) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Monophosphorylated RNAs were selectively degraded by Terminator 5’-phosphate-dependent exonuclease (Lucigen). Subsequent 5’ dephosphorylation by quickCIP (NEB ...
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bioRxiv - Genetics 2019Quote: ... 150 ng of each sample was treated with RNA 5’ polyphosphatase (Epicentre) to ensure small RNA capture independently of 5’ phosphorylation status ...
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bioRxiv - Immunology 2021Quote: ... Monophosphorylated RNAs were selectively degraded by Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen) and RNAs were 5’dephosporylation by quickCIP (NEB) ...
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bioRxiv - Genomics 2021Quote: ... and 5 electroporations were performed into Endura electrocompetent cells (Lucigen, Middleton WI) and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Genomics 2021Quote: ... Prior to incubation with Terminator™ 5′-Phosphate-Dependent Exonuclease (TEX) (Lucigen) to remove all residual RNAs containing 5’ monophosphate ...
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bioRxiv - Microbiology 2023Quote: ... unligated 5’-P RNA fragments were removed using terminator exonuclease (TEX, Lucigen), followed by ligation of transcriptional start site (TSS)-specific adaptors.