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Citations for Lucigen :
1 - 50 of 52 citations for Arsenazo I Trisodium Salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... DNase I (Epicentre) digestion was performed according to the manufacture’s instruction to minimize contamination with plasmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligase I (Lucigen) at 68°C for 2 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 6.25 units RNase I (Epicentre) was added to 5ul of sample in a total volume of 50 ul in IP buffer supplemented with 0.1% Igepal ...
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bioRxiv - Cancer Biology 2023Quote: ... 5U/uL CircLigase I (Lucigen)) at 60C for 3 hours with heat inactivation at 80C for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 8U of DNase I (Epicentre). These diluted nuclear extracts were pre-cleared using 25µL of previously blocked Streptavidin Sepharose beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
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bioRxiv - Molecular Biology 2023Quote: ... coli RNase I (10 U/µL, Epicentre) digestion occurred at room temperature for 45 minutes while lysate gently rotated or mixed ...
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bioRxiv - Molecular Biology 2023Quote: ... and 50 U/ml RNase I (Lucigen, N6901K) for 30 minutes at room temperature with gentle mixing ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 2 µL of DNase I (Lucigen) at 37°C for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... Circularization was performed with CircLigase I (Epicentre #CL4111K) instead of CircLigase II ...
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bioRxiv - Cancer Biology 2023Quote: ... RNase I/Hybridase (Lucigen, 55C for 5 minutes) and the Oligo Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Cancer Biology 2024Quote: ... and samples were circularized with CircLigase I (Lucigen). A round of rDNA depletion was performed using biotin-labeled antisense probes and Dynabeads MyOne Streptavidin C176 ...
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bioRxiv - Biochemistry 2020Quote: ... Illumina adapters were ligated using Circ Ligase I (Lucigen) and linker pA-Adapt-Bp (Table S5) ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were treated with 500 U of RNAse I (Epicentre) per 25 μg of RNA (quantified by Qubit fluorimetry – ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNAs were circularized using 100U CircLigase I ssDNA ligase (Epicentre), and cDNA concentration was quantified by QPCR of cDNA compared to a standard curve of a reference sample ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Cell Biology 2024Quote: ... We treated 10 μg of RNA with RNase I (Epicentre) for 45 min at 25°C ...
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bioRxiv - Systems Biology 2023Quote: ... the RNA products were treated with Baseline-ZERO DNase I (Epicentre) and then purified again using the RNeasy Mini kit.
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bioRxiv - Cell Biology 2022Quote: ... and treated with DNase I (cat# D9905K; Lucigen, Middleton, WI, USA). The concentration and purity of the isolated RNA were determined using a NanoDrop One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, Cat: CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Genomics 2021Quote: ... Following DNAse I digestion using the Baseline-ZERO Kit (Epicentre, Madison, Wisconsin), the RNA concentration was quantified using the Qubit RNA HS Assay Kit on a Qubit Fluorometer (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 µg/mL cycloheximide) and incubated with 15 U RNase I (Lucigen) for 45 minutes while rotating at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, cat. CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Biochemistry 2023Quote: ... and the transcription was terminated with the addition of DNase I (Lucigen, ASF3507). RNA targets were purified with Monarch® RNA Cleanup Kit (T2040L ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by reaction clean up with exonuclease I (Lucigen, 37C for 30 minutes), RNase I/Hybridase (Lucigen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates containing 10 μg of total RNA were subjected to RNase I (Lucigen) treatment for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... UHRR and HeLa S3 RNAs (1 μg) were incubated with 20 U exonuclease I (Lucigen) and 2 U Baseline-ZERO DNase (Lucigen ...
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bioRxiv - Cancer Biology 2021Quote: ... and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K) for 45 minutes at RT with light shaking ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mg of RNA (∼2 ml sample) was digested with 62.5 units of RNase I (Epicentre) at 4°C for 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... 40-60 μg of RNA from the lysate were treated with RNAse I (Lucigen, Cat. #N6901K) for 50 minutes at room temperature while rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Lysate containing 20 µg of total RNA was digested with 10 U of RNase I (Lucigen, WI, USA) for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell lysates containing 10 µg of total RNA were each treated with 12.5 units of RNase I (Epicentre) at 23°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... whole cell lysates containing 20 µg of total RNA were each treated with 10 units of RNase I (Epicentre) at 24°C for 45 min ...
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bioRxiv - Genomics 2020Quote: ... then digested with exonuclease I by adding 1 μL of enzyme (20 U/μL) plus 1.5 μL 10X Reaction Buffer (Lucigen) and incubating 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... To obtain ribosome footprints 0.12 ml of total extracts containing 300 µg of total RNA were treated with RNAse I (Epicentre) (25U/1 mg of total RNA) ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 μg of lysate was diluted to 200 μL in polysome buffer (lysis buffer without Triton X-100) and 1.5 μL RNase I was added (Epicentre #N6901K). RNase digestion was carried out at 200 rpm at 37°C for 45 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... In vitro transcribed RNA were incubated for 1h at 37°C after addition of 10units DNase I and 10units DNase Zero (Epicentre). The RNAs were then ethanol precipitated and resuspended in water ...
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bioRxiv - Molecular Biology 2020Quote: ... In vitro transcribed RNA were incubated for 1h at 37°C after addition of 10units DNase I and 10units DNase Zero (Epicentre). The RNA were then ethanol precipitated and resuspended in water and dephosphorylated using 2units Alkaline Phosphatase (FastAP ...
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bioRxiv - Molecular Biology 2024Quote: ... DNase I-treated and poly(A)-selected RNA was subjected to treatment with Terminator 5ʹ-Phosphate-Dependent Exonuclease (Epicentre; TER51020) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution (corresponding to 10 µg of total RNA) was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 minutes at a temperature of 25°C ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA pellets were washed once in 80% ethanol and pellets were air-dried and dissolved in 11uL of water, which was then circularized with the addition of 9uL of CircLigase I mix (1M betaine, 1x CircLigase Buffer (Lucigen), 2.5mM MnCl2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Biophysics 2023Quote: ... The 3′ ligation oligo (listed in Supplemental Table S6) was ligated onto the cDNA using CircLigase I (Lucigen #CL4111K, 100 units) with CircLigase Reaction Buffer ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...