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Citations for Lucigen :
1 - 50 of 59 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... coli TG1 (Lucigen, Middleton, WI, Cat#60502-02) to construct two libraries with sizes of 1 × 107 and 2 × 107 independent transformants for Green and Red ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... circularized using CircLigase II (EpiCentre), annealed to the iCLIP Cut Oligo ...
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bioRxiv - Immunology 2024Quote: ... circularised with CircLigase II (Epicentre) and PCR amplified using Solexa P5/P7 primers ...
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bioRxiv - Developmental Biology 2021Quote: ... then circularised using Circligase II (Epicentre) followed by AMPure XP beads purification ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µL circLigase II (Lucigen # CL9025K) 1.5 µL 10X circLigase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µL circLigase II (Lucigen # CL9025K) 1.5 µL 10X circLigase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 1× CircLigase II buffer (Lucigen, CL9021K), and 2.5 mM MnCl2 was added to 16 µL of cDNA and incubated at 60°C for 100 min followed by 80°C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... Next 5 μL of circularization reaction buffer was added (3.3 μL water, 1.5 μL 10x Circligase-II buffer, and 0.5 μL of Circligase-II (Epicentre)) ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was resuspended in 7 μl of water and then 8 μl of CircLigase II mix (5 μl water, 1.5 μl 10x CircLigase Buffer II, 0.75 μl 50 mM MnCl2, 0.75 μl Circligase II [Lucigen]) were added ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones that were capable of producing hydroxylamine and nitrite from ammonium were screened and sequenced using specific pCC2FOS sequencing primers (Epicentre) at SinoGenoMax (Beijing ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... and then circularized using CircLigase II (Epicentre). Two rounds of PCR were then performed to first amplify the library using adaptor-specific primers and to add sequences compatible with Illumina sequencing instruments ...
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bioRxiv - Neuroscience 2020Quote: ... and circularized with CircLigase II (Epicentre CL9025K). RPF libraries were PCR amplified with Phusion High-Fidelity DNA Polymerase (NEB M0530A ...
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bioRxiv - Genetics 2020Quote: ... and subsequently circularised with CircLigase II (Epicentre). PCR amplification was obtained after 18-21 cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... Fragments were circularized by CircLigase II (Epicentre), recovered by ethanol precipitation ...
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bioRxiv - Immunology 2024Quote: ... cDNA was circularised with CircLigase II (Epicentre), amplified by PCR using Solexa P5/P7 primers and sequenced in a DNBseq platform from BGI Genomics (100bp ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... cDNA was then circularized with CircLigase II (Epicentre) and used for library PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... circularized by CircLigase™ II ssDNA ligase (Lucigen). Subsequently ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (ii) Shotgun PCR-free library preparation kit (Lucigen) or (iii ...
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bioRxiv - Biochemistry 2024Quote: ... The Circligase II kit (CL9021K) was from Lucigen; the RNA Clean and Concentrator kit (R1019 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recovered cDNA was circularized with Circligase™ II (Epicentre) at 60 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were circularized using circligase II (#CL9025K, Epicentre). The major rRNA contaminants were removed using subtractive hybridization with custom biotinylated oligos (Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Extracted cDNA was then circularized using CircLigase II (Lucigen). Circular DNA was quantified via qPCR ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1×107 mid-log phase cells using the Masterpure Yeast RNA Purification Kit (Epicentre), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was then circularized using Circligase II (Lucigen, #CL4111K) and then linearized using BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... and the RT product was circularized by CircLigase II (Epicentre). A PCR library was amplified and sequenced using Illumina Nextseq 500 at the Rockefeller University Genomics Center.
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bioRxiv - Molecular Biology 2022Quote: ... The purified cDNAs were then ligated using CircLigase II (Lucigen) and PCR-amplified to generate the sequencing library.
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bioRxiv - Microbiology 2022Quote: ... Linear single stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Neuroscience 2022Quote: ... cDNA was size selected and circularized with Circligase II (Epicentre). Circularized cDNA was cut with BamHI and amplified using Accuprime Supermix I (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified cDNA was circularized using CircLigase II enzyme (Epicentre, CL9025K), then library PCR ...
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bioRxiv - Microbiology 2023Quote: ... Linear single-stranded cDNA was circularized using CircLigase II (Lucigen) in accordance with manufacturer instructions.
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 1 × 107 cells in exponential growth phase using the Masterpure Yeast RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from two biological replicates from early log phase growth in either YPD or YPD containing 5 mM TUDCA using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Molecular Biology 2020Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al ...
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bioRxiv - Synthetic Biology 2020Quote: ... The synthetic template DNA (IDT) was circularized using CircLigase II (Epicentre), treated with Exonuclease I (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted cDNA was circularized with CircLigase II ssDNA ligase (Lucigen, CL9021K) for 1 hr at 60°C ...
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bioRxiv - Genomics 2023Quote: ... and gel- purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described36 and libraries constructed using a different reverse indexing primer for each sample.
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bioRxiv - Immunology 2023Quote: ... Circular ligation was performed using CircLigase II ssDNA Ligase (Epicentre CL4111K) for 1,5 hours at 60°C ...
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were selected and subcultured in THM to stationary phase and genomic DNA prepared using the Masterpure Gram-positive DNA purification kit (Lucigen, Middleton, WI). The samples then were purified further using ZymoDNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was circularized by CircLigase™ II ssDNA Ligase (Epicentre, CL9025K). Next ...
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bioRxiv - Microbiology 2022Quote: ... Gel-purified cDNAs were then circularized with CircLigase II (Lucigen, Cat: CL4115K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 2.5 U of CircLigase II ssDNA Ligase (Lucigen), and incubating the resultant mixtures at 40°C for 3 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.4μL 50mM MnCl2 and 0.3μL of 100U/μl CicrLigase II ssDNA ligase (Epicentre CL9021K) and incubated in a PCR machine at 60°C for 60 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 10pmol of template oligo was cyclized and ligated using CircLigase II ssDNA Ligase (Lucigen) supplemented with 2.5mM MnCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL of circularization reaction mix containing 5 units/µL CircLigase II (Lucigen, CL9021K), 1× CircLigase II buffer (Lucigen ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were size-purified on TBE-Urea gels before being circularized by CircLigase II (Epicentre). Circularised cDNAs were then annealed to an oligonucleotide complementary to the BamHI site and then BamHI digested ...