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Citations for Lucigen :
351 - 400 of 442 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... the pellet was resuspended in the yeast cell lysis solution from the MasterPure Yeast DNA extraction kit (Epicentre) and DNA was extracted according to the kit procedure.
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bioRxiv - Cell Biology 2023Quote: Cells plated on glass coverslips were incubated with 5 µg/mL Brefeldin A (BFA) (Epicentre Biotechnologies, Madison, WI) for 30 min ...
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bioRxiv - Genetics 2023Quote: ... the Gibson reaction products were purified by isopropanol precipitation and electroporated into Endura electrocompetent cells (Lucigen, 60242-2). The transformed bacteria were spread onto LB agar plates containing 100 µg/mL ampicillin and incubated overnight at 37℃ ...
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bioRxiv - Neuroscience 2021Quote: The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell lysates containing 10 µg of total RNA were each treated with 12.5 units of RNase I (Epicentre) at 23°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... whole cell lysates containing 20 µg of total RNA were each treated with 10 units of RNase I (Epicentre) at 24°C for 45 min ...
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bioRxiv - Biochemistry 2019Quote: The DNA constructs of Loa22 were individually transformed into expression host cell E.coli ClearColiTMBL21 (DE3) pLys (Lucigen, Middleton, WI). Loa22 and its variants were grown at 37°C in the medium with 50 μg/mL ampicillin to an OD600 of approximately 0.6 ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated for 10 min at 37°C in the presence of Ready-Lyse lysozyme solution (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... These primers were used to amplify cell pools after DNA extraction using Quick Extract (Lucigen, Wisconsin, USA, Cat #QE09050). Amplified reads were sequenced on either an Illumina MiSeq or Illumina HiSeq 2500.
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bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1.2ug of ligated pUSEPR plasmid DNA per sub-pool was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37°C ...
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bioRxiv - Genomics 2022Quote: ... A total of 1.2 µg of ligated pUSEBR-CRISPR Library plasmid DNA was then electroporated into Endura electrocompetent cells (Lucigen). Competent cells were recovered for 1 h at 37ºC ...
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bioRxiv - Bioengineering 2019Quote: Cells in 96 well plates were washed once with PBS before addition of QuickExtract™ DNA Extraction Solution (Lucigen). The volume was adjusted based on the estimated cell number ...
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bioRxiv - Systems Biology 2019Quote: Ten micrograms of total RNA extracted from cell-samples at OD600 1.2 and 1.8 were treated with 5’-Terminator Dependent Exonuclease (Lucigen, TER51020) as per manufacturer instructions using Buffer A ...
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bioRxiv - Genomics 2021Quote: ... 100μg total RNA from cortex or cultured HEK293 cells was treated with or without 1μL of RNaseR [20 U/μl] (Lucigen), plus 1.9μL RNaseOUT [40 U/μL] (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Cell suspension was incubated at room temperature for 30 min with 2,500 units of Ready-Lyse lysozyme (Lucigen #R1804M) and 200 μg RNase A (Qiagen #19101) ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from 50mL flash-frozen cell pellets grown to mid-log (OD600 ∼0.65-0.85) using MasterPure™ yeast RNA purification kit (Lucigen) including a DNaseI treatment step ...
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bioRxiv - Genetics 2022Quote: ... genomic DNA of the single cell clones with good proliferation was extracted using a QuickExtract DNA extraction kit (Epicentre). The exon 23-24 region was amplified by a pair of PCR primer (SMCHD1_PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified products and the linearized N-his pETite vector were transformed in HI-Control10G Chemically Competent Cells (Lucigen) and plated on LB plates supplemented with 50 μg/ml kanamycin (Kan) ...
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bioRxiv - Systems Biology 2023Quote: ... Eight transformation reactions were conducted with 2 μl of ligation product into each tube of electrocompetent cells (Lucigen, #60242) and plated onto eight 15-cm plates with Carbenicillin selection (50 μg/ml) ...
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bioRxiv - Genomics 2023Quote: ... The resulting plasmid libraries were purified and concentrated via SPRI bead cleanup before being transformed into electrocompetent cells (Lucigen Endura ...
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bioRxiv - Bioengineering 2023Quote: ... Supernatant were carefully discarded and the isolated hair cells were lysed by 5μl QuickExtract™ DNA Extraction Solution (Lucigen) and incubated in 65°C for 6 min then 98°C 3 min ...
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bioRxiv - Genetics 2023Quote: ... cells were washed once with 500 µl PBS and genomic DNA was harvested using QuickExtract DNA extraction solution (Epicentre) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: The extracellular domains of HLA-G2 (HLA-G2) were expressed in Escherichia coli ClearColi BL21(DE3) competent cells (Lucigen) to avoid triggering endotoxic responses in human cells ...
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bioRxiv - Cell Biology 2020Quote: ... ribosomal RNA was depleted from HCT116 wild-type and KO-CREB1 cells by using a Ribo-Zero rRNA Removal Kit (Epicentre). Then ...
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bioRxiv - Cell Biology 2022Quote: ... At this stage a small aliquot of cells (75% of the plate) was lysed in 200 μL of QuickExtract DNA Extraction solution (Lucigen) and gDNA was prepared by heating to 65° C for 10 minutes followed by heat inactivation at 95° C for 2 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA was isolated from candidate RIG-I or IRF3 KO cell clones using the QuickExtract DNA extraction solution (Epicentre). Genomic DNA isolated from the RIG-I or IRF3 KO cell clones was then amplified by PCR using primers spanning exon 1 for RIG-I or exon 2 for IRF3 (see Table 1) ...
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bioRxiv - Microbiology 2019Quote: DNA of DBB and MSL71⊤ cells was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, WI, USA). The genomes were sequenced using the Illumina HiSeq2000 paired-end sequencing platform (GATC Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... a 3-axis micromanipulator with borosilicate glass electrodes was used to pick up cells into 3µL of lysis buffer (Epicentre, MessageBOOSTER), their ROI identifier was recorded ...
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bioRxiv - Immunology 2019Quote: ... washed once in PBS and genomic DNA was recovered from 1 x 106 cells using 100 μl Quick Extract solution (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Cells grown on YES solid media were used for genomic DNA preparation using the MasterPure Yeast DNA Purification Kit (Epicentre). The kit manufacturer’s protocol was followed ...
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bioRxiv - Biochemistry 2020Quote: ... ratio overnight at 16 °C followed by purification and then electroporation into phage-display competent TG-1 cells (#60502-1, Lucigen). The library was plated onto 2xYT agar plates containing 100 µg/mL carbenicillin and 2% glucose at 37 °C overnight ...
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bioRxiv - Microbiology 2021Quote: DNA isolation from inactivated cells was carried out using MasterPure™ Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from cell pellets as described (Greenstein et al 2018) using the Masterpure yeast RNA extraction kit (Lucigen). cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018 ...
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bioRxiv - Cell Biology 2021Quote: ... Selected clones lacking the expression of protein-of-interest were sequenced to confirm the knockout: cells were resuspended in QuickExtract (Lucigen), incubated at 65 °C for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligo pools were cloned into a modified pLKO-TET-ON plasmid by the Golden Gate method and the resulting product was used to transform Endura electrocompetent cells (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... at 37°C (1 – 2 h) and 0.9 μl from the reaction mix were electroporated to E.coli Cloni® 10G cells (Lucigen, USA). Colony PCR and sequencing were used for analysis and verification.
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bioRxiv - Bioengineering 2022Quote: ... a C-terminal AviTag was added to the pSANG10 expression constructs (pSANG10-Avi) and scFvs transformed into BL21[DE3] cells (Lucigen) containing the pBirAcm plasmid (Avidity).
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bioRxiv - Developmental Biology 2020Quote: ... Cell debris was then pelleted and used for gDNA extraction with 10-20 µl QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The purified PCR product was inserted into digested backbone using Gibson assembly67 followed by ethanol precipitation and transformation of electrocompetent Endura cells (Lucigen). Bacteria were plated on a large 24.5 × 24.5 cm2 plate containing solid media and ampicillin and cells were scraped from the plate for plasmid DNA extraction using a plasmid midi kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ProPeL experiments were carried out as previously described8 with the following conditions for in vivo proteome phosphorylation: all CK2 constructs were expressed in Escherichia coli OverExpress C43(DE3) cells (Lucigen) by IPTG induction ...
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bioRxiv - Bioengineering 2020Quote: ... After mutagenesis DNA sublibrary pools were digested with appropriate restriction enzymes to remove phagemid template before transformation into SS320 electrocompetent cell (Lucigen) for phage production ...
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bioRxiv - Molecular Biology 2022Quote: ... Fig 5B-D and Fig EV5 were obtained as follows: Total RNA was extracted from cells using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Epicentre), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Half of the cells in each well were used to purify genomic DNA via the Epicentre MasterPure DNA Purification Kit (Epicentre). PCR was used to amplify the CRISPR reaction locus with designed primer pairs ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from bulk cells transfected with CRISPR/Cas9 plasmids using the epicentre QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Approximately 50 microliters of the thawed Omnigene preserved stool sample was added to a microfuge tube containing 350 μL Tissue and Cell lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products) ...
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bioRxiv - Microbiology 2021Quote: ... approximately 50 microliters of the thawed Omnigene preserved stool sample was added to a microfuge tube containing 350 μL Tissue and Cell lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products).
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bioRxiv - Microbiology 2021Quote: ... culture media (Lab M) and the other one into a microfuge tube containing 350 μL Tissue and Cell Lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products ...
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bioRxiv - Immunology 2021Quote: Mice facial and back skin were swabbed at the end of the experiment using a sterile foam tipped applicator (Puritan) that was moistened with yeast cell lysis buffer (Lucigen) and collected into sterile 2 ml tubes (Eppendorf) ...