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Citations for Lucigen :
301 - 350 of 797 citations for Acetylcholinesterase Fluorescent Activity Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted using the MasterPure Yeast DNA purification kit (Epicentre, MPY80200), and concentrations were measured by Qubit ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted using the MasterPure Yeast RNA Purification Kit (Lucigen). First strand cDNA synthesis was performed using the EasyScript Plus cDNA Synthesis Kit (Lamda Biotech ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted using the MasterPure Yeast DNA Purification Kit (Epicentre). An Illumina sequencing library was prepared using home-made Tn5 transposase as previously described (Tao et al ...
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bioRxiv - Microbiology 2023Quote: Total RNA was isolated using the MasterPure Yeast RNA Purification Kit (Epicentre). cDNA was synthesized using the PrimeScript RT Master Mix (Takara).
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bioRxiv - Evolutionary Biology 2023Quote: ... shRNAs were synthesized with AmpliScribe T7-Flash Transcription Kit (Epicentre, Charlotte, NC) for 15 h followed by 15 min treatment with 1 µl of DNase I ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted using MasterPure yeast DNA purification kit (Lucigen MPY80200). A DNA fragment containing a gRNA with the tracrRNA scaffold sequence ...
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bioRxiv - Microbiology 2023Quote: ... subtilis CU1065 genomic DNA prepared using a DNA extraction kit (QuickExtract, Epicentre). PCR amplification was performed using primers YtqA_F and YtqB_R (Table S3 ...
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bioRxiv - Cell Biology 2024Quote: RNA extraction was performed using the MasterPure Yeast RNA purification kit (Lucigen) from samples treated as indicated in the legend of Figure S1 ...
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bioRxiv - Genomics 2023Quote: ... About 20,000 prepared barcode beads were resuspended in 40 μl enzyme buffer (1 U/μl Fast-Link DNA Ligase (Lucigen, E0077-2-D3), 20% End-It Enzyme Mix (Lucigen ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.
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bioRxiv - Developmental Biology 2023Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
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bioRxiv - Developmental Biology 2024Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
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bioRxiv - Biochemistry 2020Quote: ... pNT-15 was constructed using NEB HiFi DNA Assembly with a gBlock® from IDT (Coralville, IA) and cloning vector pSMART-HCKan (Lucigen, #40704-2). All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB ...
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bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using the Masterpure Yeast RNA extraction kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... the genomic DNA of edited cells were extracted using Quick Extraction kit (Lucigen). Target sites carrying gene-edited sequences were PCR amplified using gene-specific primers (Additional file 2 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... total DNA was prepared using MasterPure Complete DNA Purification Kit (Epicentre; Madison, Wisconsin). NexteraXT libraries were prepared and sequenced on an Illumina NextSeq 500 at the Genomics Core at Case Western Reserve University ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted using the MasterPure™ Yeast DNA Purification Kit (Epicentre MPY80200), with slight modifications compared to the manufacturer’s guidelines as follows ...
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bioRxiv - Microbiology 2021Quote: ... DNA precipitations were completed with a MasterPure Gram Positive DNA Purification kit (Epicentre, Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transferred to tubes containing 3uL of lysis buffer (Epicentre, MessageBOOSTER kit), and stored at - 80° ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was purified using the MasterPure Gram-positive DNA purification kit (Epicentre) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to tubes containing 3 μL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 h of removal from the incubator and within 4 h of removal from the animals.
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bioRxiv - Microbiology 2020Quote: ... and rRNA was depleted with the Illumina Ribo-Zero rRNA Removal kit (Epicentre). RNA integrity was checked with an RNA nano chip (Agilent Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... DNA was extracted from all samples using MasterPure Yeast DNA Purification Kit (Lucigen).
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated with the MasterPure™ Yeast DNA Purification Kit (Lucigen) and submitted to GENEWIZ for whole-genome sequencing ...
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bioRxiv - Immunology 2022Quote: ... and additionally purified with a mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... and the rRNA was removed with Ribo-Zero Magnetic Gold Kit (Epicentre, USA). The extracted RNA was reverse transcribed with anchored random primers ...
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bioRxiv - Microbiology 2020Quote: ... was isolated using the MasterPure DNA purification kit (MCD85201 Epicentre, Middleton, WI, USA). For constructing nanB-deletion mutants ...
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bioRxiv - Microbiology 2021Quote: ... and mRNA was enriched using the Bacterial Ribo-Zero rRNA removal kit (Epicentre). Residual mRNA was converted to sequencing libraries using TruSeq barcoded adaptors (Illumina ...
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bioRxiv - Genetics 2022Quote: Transposon mutagenesis was performed using the EZ-Tn5
transposon kit (Epicentre) and EK54/MA56/LP372 Tn5 transposase ... -
bioRxiv - Evolutionary Biology 2019Quote: ... RNA was purified using the MasterPure™ Yeast RNA Purification Kit from Epicentre. cDNA was synthesized with random hexamer primers using SuperScript® IV reverse transcriptase ...
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bioRxiv - Synthetic Biology 2019Quote: ... ssDNA adapter ligation was performed using the CircLigase ssDNA ligase kit (#CL4115K, Epicentre). Briefly ...
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bioRxiv - Genetics 2020Quote: ... ribosomal RNA was removed by Epicetre Ribozero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA contamination was removed using the Ribo-Zero Magnetic Kit (Bacteria) (Epicentre). Finally ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries were prepared using the ScriptSeqTM Complete Kit (Epicentre, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the RiboZero gold kit (Epicentre), and enriched mRNA was applied to library preparation according to manufacturer’s protocol (NEBNext Ultra) ...
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bioRxiv - Genetics 2021Quote: ... DNA was end repaired by using End-It- DNA End-Repair Kit (Epicentre) and linker ligated with T4 ligase (NEB) ...
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bioRxiv - Microbiology 2019Quote: ... The Ribo-Zero™ rRNA Removal Kit (Meta-Bacteria; Epicentre, Madison, WI, USA) was used to deplete total RNA of the ribosomal RNA ...
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bioRxiv - Genomics 2019Quote: ... Gram-negative and Human/mouse/rat Ribo-Zero rRNA Removal Kit (Epicentre Technologies). The resulting RNA was purified using Zymo RNA clean & Contentrator-5 column kit (ZYMO Research) ...
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bioRxiv - Genomics 2021Quote: ... and DNA extraction was carried out with MasterPure Complete DNA Isolation kit (Lucigen) as indicated by the manufacturer ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was prepared using MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was prepared using MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... or Epicenter MasterPure Complete DNA and RNA Purification Kit (Lucigen, Madison, WI, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), for PacBio sequencing ...
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bioRxiv - Microbiology 2021Quote: ... ScriptSeq™ v2 RNA-Seq library preparation kit (Epicentre, Illumina, Madison, WI USA) was used for this purpose ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was extracted using the MasterPure Yeast DNA Purification Kit (Epicentre, UK). During the extraction protocol ...
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bioRxiv - Genomics 2022Quote: ... Purified ChIP DNA was repaired with End-It DNA End-Repair Kit (Epicentre). The library was then indexed and amplified and sequenced on an Illumina platform ...
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bioRxiv - Molecular Biology 2022Quote: ... and inserted into pJAZZ-OK using BigEasy® v2.0 Linear Cloning Kit (Lucigen) to increase plasmid copy number (Figure S3b) ...
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bioRxiv - Plant Biology 2022Quote: ... whereas ribosomal RNA was removed using a Ribo-Zero rRNA Depletion Kit (Epicentre). Strand-specific RNA-Seq libraries were synthesized using a Plant Leaf ScriptSeq Complete Kit (Epicentre) ...