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Citations for Lucigen :
151 - 182 of 182 citations for 7 Oxabicyclo 4.1.0 heptan 2 one 4 4 6 trimethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... A library of ∼500,000 independent mini-Tn5 transposon mutants was created by transforming BW25113 ΔascA with an EZ-Tn5
2> Tnp transposome kit (Epicentre) by electroporation ... -
bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.
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bioRxiv - Genomics 2021Quote: ... 1 μl of the Gibson reaction was delivered to 25 μl of electrocompetent EnduraTM cells (Lucigen, cat. no. 60242-2) using Gene Pulser®/MicroPulser™ Electroporation Cuvettes ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor ...
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bioRxiv - Immunology 2021Quote: Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
2>Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... was constructed from the linear pSMART-HCKan vector supplied in the Lucigen CloneSmart kit (Lucigen, Middleton WI, Cat#40708-2) with a single ligation step ...
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bioRxiv - Microbiology 2023Quote: Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5
2> Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ... -
bioRxiv - Microbiology 2023Quote: Tn-5 transposon insertion library was build based on EZ-Tn5™
2>Tnp transposome system (Lucigen, WI, USA). Competent C ... -
bioRxiv - Developmental Biology 2021Quote: ... In brief, RNA was treated with 20 μl RNase R digestion reaction (2 μg RNA, 1 μl RNase R (Lucigen, #RNR07250), 2 μl 10x RNase R reaction buffer ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Neuroscience 2019Quote: ... The samples were then pelleted at 500rcf for 10 min at 4C followed by a wash in 10mL of Nuclei Wash & Resuspension Buffer (1X PBS, 1% BSA, 0.2U/ul NxGen RNase inhibitor (Lucigen, 30281-2)) ...
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bioRxiv - Biochemistry 2022Quote: ... the genes encoding the three isoforms of hnRNPDL (hnRNPDL-1, hnRNPDL-2 and hnRNPDL-3) were inserted into pETite (Lucigen corporation) vector with a His-SUMO N-terminal tag ...
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bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
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bioRxiv - Evolutionary Biology 2023Quote: A transposon mutant library for the WT strain was generated by electroporation using the EZ-Tn5
2> Tnp transposome kit (Lucigen Inc.). The library consisted of approximately 40,000 insertion mutants ... -
bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
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bioRxiv - Genomics 2023Quote: ... About 20,000 prepared barcode beads were resuspended in 40 μl enzyme buffer (1 U/μl Fast-Link DNA Ligase (Lucigen, E0077-2-D3), 20% End-It Enzyme Mix (Lucigen ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.
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bioRxiv - Developmental Biology 2023Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
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bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
2> Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Developmental Biology 2024Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
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bioRxiv - Biochemistry 2020Quote: ... pNT-15 was constructed using NEB HiFi DNA Assembly with a gBlock® from IDT (Coralville, IA) and cloning vector pSMART-HCKan (Lucigen, #40704-2). All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
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bioRxiv - Microbiology 2021Quote: ... filled with 1 ml LB supplemented with 25 μg/ml chloramphenicol and 2 µl/ml CopyControl fosmid autoinduction solution (Epicentre Biotechnologies, Cat. No. AIS107F). The plates were grown at 30 °C shaking at 700 RPM for 16 h covered by an AeraSeal gas-permeable sheet (EXCEL Scientific Cat ...