Labshake search
Citations for Lucigen :
1 - 50 of 223 citations for 7 Chlorofuro 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... 2) Poly(A)-tailing to add adenosines to the open 3’ ends of RNA (Lucigen, #PAP5104H), and 3 ...
-
bioRxiv - Biochemistry 2024Quote: ... and TetraCys-tagged LexAPaCTD were amplified from the genomic DNA using primers LexA_Pa.For/Rev and LexA_Pa_CTD_4Cys.For/Rev (Supplementary Table 3) and cloned in pETite C-His Kan vector and pETite N-His SUMO Kan Vector (Lucigen), respectively ...
-
bioRxiv - Cell Biology 2021Quote: Total RNAs was treated with RNase R for 30 min at 37°C using 3 U/mg of RNase R (Lucigen, USA). HCC-LM3 and Huh-7 cells treated with actinomycin D (1 µg/mL ...
-
bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
-
bioRxiv - Molecular Biology 2024Quote: Step 2 - extension & ligation: 10 μl of pre-heated (60°C) extension/ligation mix containing 5U of ampligase (Lucigen), 4U of Phusion HF DNA polymerase (#F530S ...
-
bioRxiv - Molecular Biology 2020Quote: Rolling Cycle Amplification (RCA) was done O/N at 30°C using 0.5 Units/μl Φ29 polymerase (Lucigen, 30221-2). The reaction mixture contained also 1X Φ29 buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... the genes encoding the three isoforms of hnRNPDL (hnRNPDL-1, hnRNPDL-2 and hnRNPDL-3) were inserted into pETite (Lucigen corporation) vector with a His-SUMO N-terminal tag ...
-
bioRxiv - Developmental Biology 2023Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
-
bioRxiv - Developmental Biology 2024Quote: ... at 25°C for 16 hours followed by RCA (rolling cycle amplification) at 30°C for 16 hours by using phi29 polymerase (Lucigen, 30221–2) and RCA primer1 ...
-
bioRxiv - Microbiology 2022Quote: ... The pilT gene and flanking sequence from the FA1090 chromosome was amplified by PCR with primers PilT-F 5’-CATTGAGGTCGGCAAGCAGC-3’ and PilT-R 5’-GCATCTTTACCCAGCGCGAAAT-3’ and cloned into pSMART HC Kan (Lucigen). The cloning mix was transformed into TOP10 E ...
-
bioRxiv - Molecular Biology 2022Quote: ... The 3’end of resultant cDNAs were ligated to a ssDNA linker (5’-PhosNNNAGATCGGAAGAGCGTCGTGTAG-/3SpC3/3’) using Circligase ssDNA Ligase (Epicentre) at 65°C for 12 hrs ...
-
bioRxiv - Genetics 2021Quote: ... coli (Lucigen, 60242-2) by electroporation (1.8 kV ...
-
bioRxiv - Neuroscience 2022Quote: ... coli (Lucigen 60242-2) using program EC1 on MicroPulser Electroporator (Bio-Rad 1652100 ...
-
bioRxiv - Microbiology 2021Quote: ... 3-5 μl Hybridase™ Thermostable RNase H (Lucigen) and 7 μl 10x RNase H buffer preheated to 45°C was added ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 uL of eluent was then electroporated into each of 2 tubes of 50 uL Endura electrocompetent cells (Lucigen, Cat#60242-2) as per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... coli (Lucigen, cat. 60242-2) using Bio-Rad MicroPulser Electroporator (cat ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Step 2: Riboshredder RNase (Epicentre) was used in place of RNase A ...
-
bioRxiv - Immunology 2020Quote: ... coli (Lucigen, Cat# 60502-2) over fifty shocks for each library ...
-
bioRxiv - Microbiology 2024Quote: ... 2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... Library #3 was amplified using pyrophage polymerase (Lucigen, Middleton, WI).
-
bioRxiv - Molecular Biology 2023Quote: ... and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen). Between 50 and 75 ng were retrotranscribed in cDNA with 1.5 µL of template-switching TGIRT enzyme ...
-
bioRxiv - Bioengineering 2020Quote: ... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
-
bioRxiv - Molecular Biology 2021Quote: ... were used to in vitro transcribe a 309-nt actin RNA transcript that was subsequently cap-labeled using recombinant VACV guanylyltransferase/guanine-7-methyltransferase (Epicentre Biotechnologies) in conjunction with capping buffer (50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1 ml of 37 °C Recovery Medium (Lucigen, 80026-1) was added and bacteria were grown in round-bottom tubes for 1 h at 37 °C while shaking at 180 r.p.m ...
-
bioRxiv - Microbiology 2023Quote: ... and were cloned into the pETite C-His vector (Lucigen) with a C-terminal hexahistidine tag ...
-
bioRxiv - Systems Biology 2019Quote: ... The purified capped RNA was subjected to 2’-O methylation using ScriptCapTM 2’-O-Methyltransferase (Epicentre) in the presence of cold S-adenosyl methionine (SAM ...
-
bioRxiv - Microbiology 2020Quote: ... 2 µl TEX reaction buffer (Lucigen) and 0.5 µl RiboGuard RNase Inhibitor (Lucigen ...
-
bioRxiv - Microbiology 2019Quote: ... 2 μL of EZ-Tn5 (Lucigen) in vitro-assembled transposomes were added to the 50 μL cell suspension in sorbitol before electroporation ...
-
bioRxiv - Genomics 2020Quote: ... 2 mL of recovery buffer (Lucigen) was added ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2 μl Plasmid-Safe Enzyme (Lucigen), and 6 μl 10mM ATP (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 µl of Thermostable RNAseH (Epicentre) and 8 µl of RNAseH buffer (pH 7.5 ...
-
bioRxiv - Systems Biology 2022Quote: Endura Electrocompetent Cells (Lucigen #60242-2) were thawed on ice for 10 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... v.2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions and quantified with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... Version 2 (Lucigen, Middelton, WI, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: DNA was extracted 3 dpt using QuickExtract DNA Extraction Solution (Lucigen) and heated at 65□ for 20 min followed by 95□ for 20 min ...
-
bioRxiv - Genomics 2022Quote: ... and 2 U Baseline-ZERO DNase (Lucigen) in Baseline-ZERO DNase Buffer for 30 min at 37 °C ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2 u/µl NxGen RNase inhibitor (Lucigen), 0.1 u/µl vaccinia capping enzyme (VCE ...
-
bioRxiv - Microbiology 2022Quote: ... 2 ml/L fosmid autoinduction solution (Epicentre), each plate supplemented with 0.3% (v/v ...
-
bioRxiv - Bioengineering 2023Quote: ... coli cells (Lucigen, catalog no. 60242-2) at 50–100 ng/ul ...
-
bioRxiv - Microbiology 2023Quote: ... 2 µL TypeOne Restriction Inhibitor (Lucigen, USA) and 0.4 µL transposome ...
-
bioRxiv - Systems Biology 2023Quote: ... and recovered for one hour at 37°C in Recovery Media (Lucigen). Aliquots from the transformations were used to inoculate overnight cultures of LB containing 25 μg/mL of Zeocin (ThermoFisher) ...
-
bioRxiv - Neuroscience 2020Quote: ... and transferred to tubes containing 3 μL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 h of removal from the incubator and within 4 h of removal from the animals.
-
bioRxiv - Biochemistry 2019Quote: ... the dissolved cDNA was mixed with 3 μl of 10x CircLigase Buffer (Epicentre), 1.5 μl of 50 mM MnCl2 ...
-
bioRxiv - Plant Biology 2022Quote: ... It was then proceeded using RNase R (3 U/μg; Epicentre, Madison, USA) at 37 °C ...
-
bioRxiv - Genomics 2021Quote: ... 11 μL purified CUT&Tag-DNA was used in the reaction (11 μL DNA, 2 μL 10 μM Tn5mC-ReplO1 oligo, 2 μL 10× Ampligase buffer (Lucigen), 2 μL dNTP mix (2.5mM each ...
-
bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2023Quote: ... 2 μl per tube was transformed into two tubes of 50 μl of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... The commercial transposome R6Kγori/KAN-2 from Lucigen kit #TSM08KR was used according to the manufacturer’s instructions.
-
bioRxiv - Genetics 2020Quote: ... cloni 10G SUPREME Electrocompetent Cells (Lucigen, #60080-2), in 11 parallel transformations (1 mL each ...