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Citations for Lucigen :
101 - 150 of 181 citations for 6 ETHYL 4 METHYL PYRAN 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The library was transformed into electrocompetent Lucigen Endura™ Escherichia coli (Lucigen; cat. 60242-2) using a Bio-Rad MicroPulser Electroporator (#1652100) ...
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bioRxiv - Molecular Biology 2020Quote: Transposon mutant libraries were constructed using the EZ-Tn5
2>TnP Transposome Kit (Epicentre) according to the manufacturer’s instructions ... -
bioRxiv - Microbiology 2020Quote: ... end-repaired DNA from PoV-01B (1-2 Kb) were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI ...
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bioRxiv - Microbiology 2022Quote: ... Enteritidis strains P125109 and D7795 using the EZ-Tn5™
2> Insertion Kit (Lucigen) as previously described [31] ... -
bioRxiv - Molecular Biology 2023Quote: ... we utilized the ScriptCap m7G Capping System and ScriptCap 2’-O-methyltransferase kit (Epicentre Biotechnologies) with [α-32P] GTP for our experiments ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg of purified RNA was depleted of ribosomal RNA using a Ribo-Zero rRNA Removal Kit - Plant Leaf (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments in the range of 30-40 kbp were resolved by gel electrophoresis (2 V cm-1 overnight at 4 °C) and recovered from 1% low melting point agarose gel using GELase 50X buffer and GELase enzyme (Epicentre). Nucleic acid fragments were then ligated to the linearized CopyControl pCC2FOS vector following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 2 µl water and subjected to electroporation into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and in vitro amplified using a TargetAmp 2-round aRNA Amplification Kit 2.0 (Epicentre, Madison, WI). RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent ...
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bioRxiv - Immunology 2021Quote: ... 40 nanograms of each library were transformed into Endura™ ElectroCompetent Cells (Lucigen cat 60242-2) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 2) Poly(A)-tailing to add adenosines to the open 3’ ends of RNA (Lucigen, #PAP5104H), and 3 ...
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bioRxiv - Cancer Biology 2023Quote: ... the pBA439_UMI20 library was electroporated into 25 μl of Lucigen Endura ElectroCompetent Cells (Lucigen, 60242-2) using prechilled 1 mm electroporation cuvettes (BioRad ...
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bioRxiv - Microbiology 2023Quote: Transposon library of Salmonella was generated using EZ-Tn5
2>Tnp Transposome kit (Lucigen, TSM99K2) following manufacturer’s instructions ... -
bioRxiv - Molecular Biology 2023Quote: ... 20 mg of RNA (∼2 ml sample) was digested with 62.5 units of RNase I (Epicentre) at 4°C for 1 hr ...
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bioRxiv - Microbiology 2024Quote: A transposon mutant library in pJBG007 was made using EZ-Tn5
2> Insertion Kit (Lucigen). The following components were used for a 10µl reaction (EZ-Tn5 10X Reaction Buffer (1µl ... -
bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
2> Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Microbiology 2021Quote: ... 100µl of competent cells was mixed with 1 µl of EZ-Tn5 KAN-2 Tnp Transposome (Epicentre) and electroporated at 1800V ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... were used to amplify lnt that had been disrupted by the insertion of Tn5
2> (Epicentre) [6] ... -
bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
2> Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Neuroscience 2021Quote: ... We used 1.5 μL of the ligation mix and electroporated 30 μL competent bacteria (Lucigen, 60242-2) with ...
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bioRxiv - Genetics 2020Quote: ... 2 µl per tube was transformed into two tubes of 50 µl of electrocompetent cells (Lucigen DUO) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA of 2 mL overnight culture was extracted with the MasterPure™ Yeast DNA Purification Kit (Lucigen) and whole PCR Tag analysis was performed on population level to test the general presence of all tRNAs within the population (data not shown) ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
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bioRxiv - Neuroscience 2019Quote: ... held by a 4-axis micromanipulator under bright-field optics and transferred to tubes containing 3uL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 hour of removal from the incubator and within 4 hours of removal from the animals.
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bioRxiv - Microbiology 2019Quote: ... The cells in glycerol were electroporated with the EZ-Tn5 R6Kγori/KAN-2>Tnp transposon (Epicentre, Madison WI) using an Eppendorf Electroporator 2510 at 2.5 kV yielding around 5 ms ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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bioRxiv - Microbiology 2021Quote: ... coli chromosome was carried out by electroporating 1 μL of the EZ-Tn5
2> Tnp Transposome (Epicentre) in 50 μL of EV18-pkD46 electrocompetent cells ... -
bioRxiv - Neuroscience 2021Quote: ... we electroporated 1 μL of 25 ng/μL of each library into 50 μL bacteria (Lucigen, 60242-2), with ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Clones were identified ∼2 weeks after sorting and screened by genomic DNA extraction with Quickextract (Lucigen cat.#QE09050) and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712) ...
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bioRxiv - Microbiology 2021Quote: ... Transposon mutagenesis was performed with the EZ-Tn5
2>Tnp Transposome kit (Epicentre, Illumina, CA, USA) and the insertion site of all mutants was determined via arbitrary PCR (Segev et al ... -
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Genetics 2023Quote: ... the Gibson reaction products were purified by isopropanol precipitation and electroporated into Endura electrocompetent cells (Lucigen, 60242-2). The transformed bacteria were spread onto LB agar plates containing 100 µg/mL ampicillin and incubated overnight at 37℃ ...
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Neuroscience 2022Quote: ... The puck was immersed in 200 µL of hybridization buffer (6X SSC, 2 unit / µL Lucigen NxGen RNAse inhibitor) for 30 minutes at 37°C for the binding of RNA to the oligos ...
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bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.
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bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Eight transformation reactions were conducted with 2 μl of ligation product into each tube of electrocompetent cells (Lucigen, #60242) and plated onto eight 15-cm plates with Carbenicillin selection (50 μg/ml) ...
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bioRxiv - Molecular Biology 2024Quote: Step 2 - extension & ligation: 10 μl of pre-heated (60°C) extension/ligation mix containing 5U of ampligase (Lucigen), 4U of Phusion HF DNA polymerase (#F530S ...
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bioRxiv - Genetics 2024Quote: ... The supernatant was discarded and 1.5mL Buffer D (0.01% BSA in 1X PBS with 0.5U/uL RNase inhibitor [Lucigen 30281-2]) was gently added to the nuclei pellet and incubated on ice 20min ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Molecular Biology 2020Quote: Rolling Cycle Amplification (RCA) was done O/N at 30°C using 0.5 Units/μl Φ29 polymerase (Lucigen, 30221-2). The reaction mixture contained also 1X Φ29 buffer ...
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bioRxiv - Genomics 2020Quote: Libraries were prepared from the DNA samples using the NxSeq AmpFREE Low DNA Library kit (Lucigen, Cat no. 14000-2) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... was used to create a fusion PCR product to replace fHbp in strain L91543 with the kanamycin resistance gene (kan) from EZ::Tn5
2> insertion kit (Epicentre) following the approach described by Horton [57] ... -
bioRxiv - Bioengineering 2022Quote: ... at 37°C (1 – 2 h) and 0.9 μl from the reaction mix were electroporated to E.coli Cloni® 10G cells (Lucigen, USA). Colony PCR and sequencing were used for analysis and verification.
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bioRxiv - Molecular Biology 2020Quote: ... A library of ∼500,000 independent mini-Tn5 transposon mutants was created by transforming BW25113 ΔascA with an EZ-Tn5
2> Tnp transposome kit (Epicentre) by electroporation ... -
bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.