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Citations for Lucigen :
51 - 100 of 298 citations for 6 Chloro 5 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... coli (Lucigen, cat. 60242-2) using Bio-Rad MicroPulser Electroporator (cat ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Step 2: Riboshredder RNase (Epicentre) was used in place of RNase A ...
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bioRxiv - Immunology 2020Quote: ... coli (Lucigen, Cat# 60502-2) over fifty shocks for each library ...
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bioRxiv - Microbiology 2024Quote: ... 2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Library #3 was amplified using pyrophage polymerase (Lucigen, Middleton, WI).
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bioRxiv - Molecular Biology 2023Quote: ... and 3’ ends were dephosphorylated with T4 polynucleotide kinase (Lucigen). Between 50 and 75 ng were retrotranscribed in cDNA with 1.5 µL of template-switching TGIRT enzyme ...
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bioRxiv - Microbiology 2020Quote: ... the RNA fragments were poly(A)-tailed and 5’PPP structures were removed with RNA 5’ Polyphosphatase (Epicentre). The RNA sequencing adapter with the barcodes were ligated to the 5’-monophosphate of the fragments ...
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bioRxiv - Neuroscience 2020Quote: ... coli 5-alpha Chemically Competent cells (Lucigen), performing a thermal shock for 45 seconds at 42°C followed by 2 minutes on ice ...
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bioRxiv - Biochemistry 2019Quote: ... transcripts were treated with 5’-polyphosphatase (Epicentre) and Xrn1 (New England Biolabs ...
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bioRxiv - Biochemistry 2019Quote: ... transcripts were treated with 5’-polyphosphatase (Epicentre) and Xrn1 (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and Terminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Molecular Biology 2023Quote: Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen) was used to enrich the 5′ ends of the transcripts ...
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bioRxiv - Genomics 2019Quote: ... and the 5’P of capped molecules was exposed by treatment with 5 units of Tobacco Acid Pyrophosphatase (Epicentre). RNA samples were ligated with the TIF-seq DNA/ RNA 5oligo cap using T4 RNA ligase 1 (NEB) ...
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bioRxiv - Systems Biology 2019Quote: ... The purified capped RNA was subjected to 2’-O methylation using ScriptCapTM 2’-O-Methyltransferase (Epicentre) in the presence of cold S-adenosyl methionine (SAM ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl TEX reaction buffer (Lucigen) and 0.5 µl RiboGuard RNase Inhibitor (Lucigen ...
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bioRxiv - Microbiology 2019Quote: ... 2 μL of EZ-Tn5 (Lucigen) in vitro-assembled transposomes were added to the 50 μL cell suspension in sorbitol before electroporation ...
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bioRxiv - Genomics 2020Quote: ... 2 mL of recovery buffer (Lucigen) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μl Plasmid-Safe Enzyme (Lucigen), and 6 μl 10mM ATP (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of Thermostable RNAseH (Epicentre) and 8 µl of RNAseH buffer (pH 7.5 ...
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bioRxiv - Systems Biology 2022Quote: Endura Electrocompetent Cells (Lucigen #60242-2) were thawed on ice for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... v.2 (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions and quantified with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Version 2 (Lucigen, Middelton, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: DNA was extracted 3 dpt using QuickExtract DNA Extraction Solution (Lucigen) and heated at 65□ for 20 min followed by 95□ for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then poly(A)-tailed using poly(A) polymerase and the 5’-PPP was removed using 5’ polyphosphatase (Epicentre). RNA adaptors were then ligated and synthesis of first strand cDNA was performed using M-MLV reverse transcriptase and oligo(dT ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 µl Hybridase (Lucigen, H39500, 5 units/µl) and 2.5 µl water were added to the mix ...
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bioRxiv - Microbiology 2021Quote: ... samples were treated with RNA 5‘ polyphosphatase (Lucigen). The 5‘ Illumina TruSeq sequencing adapter carrying sequence tag TAATGCGC was ligated to newly formed 5‘-P RNA ends ...
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bioRxiv - Cell Biology 2021Quote: ... cloni 5-alpha (short: E. cloni, Lucigen corporation) for all cloning procedures ...
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bioRxiv - Molecular Biology 2021Quote: ... and T erminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... we treated these samples with 5’ polyphosphatase (Lucigen) (20 U per 5 μg of RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... RNase I/Hybridase (Lucigen, 55C for 5 minutes) and the Oligo Clean and Concentrator Kit (Zymo) ...
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bioRxiv - Genomics 2022Quote: ... and 2 U Baseline-ZERO DNase (Lucigen) in Baseline-ZERO DNase Buffer for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 u/µl NxGen RNase inhibitor (Lucigen), 0.1 u/µl vaccinia capping enzyme (VCE ...
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bioRxiv - Microbiology 2022Quote: ... 2 ml/L fosmid autoinduction solution (Epicentre), each plate supplemented with 0.3% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... coli cells (Lucigen, catalog no. 60242-2) at 50–100 ng/ul ...
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bioRxiv - Microbiology 2023Quote: ... 2 µL TypeOne Restriction Inhibitor (Lucigen, USA) and 0.4 µL transposome ...
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bioRxiv - Neuroscience 2020Quote: ... using Inpp5e WT forward primer 5’-GAGAAGCTGATAGATGGCTAGG and Inpp5e WT reverse primer 5’-AACCAGAAGACCTCATCAAACC and EconoTaq PCR according to manufactures specifications (Lucigen corporation, WI) (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to tubes containing 3 μL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 h of removal from the incubator and within 4 h of removal from the animals.
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bioRxiv - Biochemistry 2019Quote: ... the dissolved cDNA was mixed with 3 μl of 10x CircLigase Buffer (Epicentre), 1.5 μl of 50 mM MnCl2 ...
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bioRxiv - Plant Biology 2022Quote: ... It was then proceeded using RNase R (3 U/μg; Epicentre, Madison, USA) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Genomics 2021Quote: ... 11 μL purified CUT&Tag-DNA was used in the reaction (11 μL DNA, 2 μL 10 μM Tn5mC-ReplO1 oligo, 2 μL 10× Ampligase buffer (Lucigen), 2 μL dNTP mix (2.5mM each ...
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bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μl per tube was transformed into two tubes of 50 μl of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The commercial transposome R6Kγori/KAN-2 from Lucigen kit #TSM08KR was used according to the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: ... cloni 10G SUPREME Electrocompetent Cells (Lucigen, #60080-2), in 11 parallel transformations (1 mL each ...
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bioRxiv - Neuroscience 2022Quote: ... and 0.1% NxGen RNAse inhibitor (Lucigen; 30281-2)) ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated with 2 µL of DNase I (Lucigen) at 37°C for 15 minutes ...
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bioRxiv - Genomics 2019Quote: ... The DNA was inserted into the vector pIndigoBAC-5 (Epicentre, Madison ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were treated with Terminator 5′ phosphate-dependent exonuclease (Epicentre) to remove RNAs with 5′ monophosphate (5′ P ...
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bioRxiv - Molecular Biology 2021Quote: ... Each aliquot was either enzymatically treated with 5’ polyphosphatase (Lucigen) (20 U per 5 μg of RNA ...