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Citations for Lucigen :
51 - 67 of 67 citations for 2x SYBR Green qPCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 1 μg purified RNA was applied to RT-qPCR and the remaining was subjected to ribosomal RNA depletion using a Ribo-Zero Gold Kit (Epicentre). Sequencing libraries were constructed from 100 ng total RNA using a Truseq stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Cell Biology 2022Quote: ... listed in Table S2 or previously described [89] and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre). All PCR amplified products were confirmed by sequencing (Eurofins MWG Operon ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 500uL of the cultured cells were pelleted and the growth media was removed. DNA was extracted using the Master Pure DNA purification kit (Cat No. 85200) from Epicentre. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L ...
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bioRxiv - Neuroscience 2021Quote: ... We used 1.5 μL of the ligation mix and electroporated 30 μL competent bacteria (Lucigen, 60242-2) with ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from mice faecal pellets obtained prior to RVwa inoculation from all experimental groups with the Master Pure™ kit (Epicentre), including a bead beater treatment with 0.5 g of 0.1 mm glass beads in the lysis step (FastPrep 24-5G Homogenizer ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA template was incubated at 37°C overnight in a transcription mix containing: transcription buffer 1X (Epicentre Biotechnologies), 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP ...
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bioRxiv - Molecular Biology 2024Quote: Step 2 - extension & ligation: 10 μl of pre-heated (60°C) extension/ligation mix containing 5U of ampligase (Lucigen), 4U of Phusion HF DNA polymerase (#F530S ...
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bioRxiv - Molecular Biology 2019Quote: ... Depletion of rRNA from the purified total RNA was done by using a mix of antisense oligonucleotides matching Drosophila melanogaster rRNAs (listed in Table S14) and the Hybridase Thermostable RNase H (Epicentre) which specifically degrades RNA in RNA-DNA hybrids ...
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bioRxiv - Genomics 2019Quote: ... After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme, CL9025K, Epicentre) was added to the sample and incubated for two hours at 60° C ...
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bioRxiv - Bioengineering 2022Quote: ... at 37°C (1 – 2 h) and 0.9 μl from the reaction mix were electroporated to E.coli Cloni® 10G cells (Lucigen, USA). Colony PCR and sequencing were used for analysis and verification.
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µg of total RNA was mixed with 3 µl of diluted ERCC RNA spike-in mix and then digested for 1h at 30°C with 1 unit of Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in 1X Reaction Buffer A containing 10 units of SUPERase-In RNase inhibitor (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA pellets were washed once in 80% ethanol and pellets were air-dried and dissolved in 11uL of water, which was then circularized with the addition of 9uL of CircLigase I mix (1M betaine, 1x CircLigase Buffer (Lucigen), 2.5mM MnCl2 ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and primers outlined in Table S1. E. coli colony PCRs (Fig. 1F and Fig. S1F- H) were performed with CloneID 1X Colony PCR Mix (Lucigen 30059-2) and primers outlined in Table S1.