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Citations for Lucigen :
401 - 444 of 444 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... culture media (Lab M) and the other one into a microfuge tube containing 350 μL Tissue and Cell Lysis buffer (Epicentre) and 100 μg 0.1 mm zirconia beads (BioSpec Products ...
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bioRxiv - Immunology 2021Quote: Mice facial and back skin were swabbed at the end of the experiment using a sterile foam tipped applicator (Puritan) that was moistened with yeast cell lysis buffer (Lucigen) and collected into sterile 2 ml tubes (Eppendorf) ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of the Gibson reaction was delivered to 25 μl of electrocompetent EnduraTM cells (Lucigen, cat. no. 60242-2) using Gene Pulser®/MicroPulser™ Electroporation Cuvettes ...
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bioRxiv - Immunology 2021Quote: Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... the electroporated T cells were pelleted and gDNA was isolated by adding 50 μL/well QuickExtract DNA extraction solution (Epicentre), followed by incubation at 37 °C for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: Approximately 1e+06 cells were harvested two days post nucleofection and incubated in 200 μL of QuickExtract DNA Extraction Solution (Lucigen) at 65ºC for 15 minutes ...
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bioRxiv - Systems Biology 2022Quote: ... 2 ul per tube was transformed into two tubes of 50 ml of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
bioRxiv - Genomics 2023Quote: ... with the following modifications as previously published37: Swabs were placed in tubes containing 350 ul Tissue and Cell Lysis solution (Lucigen), 1.5 mg/ml lysozyme (Sigma Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in 0.5 X PBS-RI and resuspended in 75 μL of 1X Ampligase buffer (Lucigen, A0102K). Pooled cells were counted and examined on the hemocytometer and diluted for loading onto the Chromium Controller (10x Genomics) ...
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bioRxiv - Developmental Biology 2023Quote: Genomic DNA of Pkd1-/-or Pkd2-/- single cell clonal mNPC lines were extracted using QuickExtractTM DNA Extraction Solution (Lucigen, # QE09050). PCR primers were designed to flank the sgRNA targeting sites of Pkd1 or Pkd2 genes (listed below) ...
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bioRxiv - Molecular Biology 2023Quote: ... which has a tetracycline resistance gene linked to the F+ gene was transformed via electroporation by incubating ER2738 electrocompetent cells (Lucigen) with ligated DNA in chilled electroporation cuvettes (0.1 cm gap ...
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bioRxiv - Genomics 2023Quote: ... 200µL of the cell suspension was washed twice in PBS and genomic DNA was extracted with Quickextract solution (Lucigen, QE0905T) according to the manufacturer’s protocol.
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bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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bioRxiv - Genomics 2023Quote: ... Skin was sampled with an established protocol using pre-moistened Puritan foam swabs collected and stored in 100 µL Yeast Cell Lysis Buffer (Lucigen) buffer at -80° and shipped on dry ice ...
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bioRxiv - Systems Biology 2023Quote: ... 2 μl per tube was transformed into two tubes of 50 μl of Endura electrocompetent cells (Lucigen, Cat#60242-2) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: DNA was isolated from fixed and sorted cells using the MasterPure DNA purification kit (MC85200, Lucigen, LGC Ltd, Teddington, UK) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Single cell–derived clonal cell lines were analyzed and genotyped by PCR using genomic DNA isolated with QuickExtract DNA Extraction Solution (Lucigen) and primers binding within and downstream the modified region (Primer 1 ...
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bioRxiv - Microbiology 2024Quote: ... and ligated into a pHEN2 vector modified with a triple c-Myc tag108 which was used to transform electrocompetent TG1 cells (Lucigen). The resulting Nb-library consisted of 5×107 independent colonies ...
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bioRxiv - Genetics 2021Quote: ... the genomic DNA of the pool of cells carrying insertion events was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Cells were lysed using a lysis solution supplemented in zymolyase 20T (1.5 mg/ml) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We concentrated the reactions by ethanol precipitation and transformed them into 100 μL of TransforMax™ EC100D™ pir+ cells (Lucigen). We resuspended the transformed cells in 1 mL of SOC medium ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Microbiology 2021Quote: ... OmRV-fragment2/pMA (ampicillin resistant) and pACYC177 low-copy-number plasmids were transformed into 10G chemically competent cells (Lucigen, E. cloni), respectively ...
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bioRxiv - Molecular Biology 2021Quote: DNA was isolated from HTT Q71-GFP-expressing HEK293 cells through MasterPure™ Complete DNA and RNA Purification Kit (Epicentre®) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Plant Biology 2023Quote: ... Thirty thousand cells of each clone were added to 100 μL of Quick Extract™ DNA Extraction Solution (Lucigen, Wisconsin, USA). DNA was extracted by heat treatment (65°C for 6 min and 98°C for 2 min) ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were counted and 1e6 cells for each sample were collected and washed with PBS before RNA were extracted by QuickExtract RNA Extraction Solution (Lucigen, QER090150). SuperScript IV VILO Master Mix (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from 2.5×105 Neuro2A cells with 250 μL of QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA) or from 30 mg of mouse liver using a EZ-10 Spin Column Animal Genomic DNA Miniprep Kit (Bio Basic ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA for CRISPR/Cas9 genotyping was isolated from cell lines and organoids by using Quick Extract DNA Extraction Solution (Lucigen, #QE09050). For organoids ...
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bioRxiv - Molecular Biology 2021Quote: ... whole genome sequencing was performed on DNA isolated from approximately 30 OD600 yeast cells using Lucigen MasterPure Yeast DNA Purification kit (Lucigen catalog: MMPY80200) according to the manufacturer’s directions ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligation products were purified by removing the enzymes and transformed into 500 μl of electroporation competent TG1 cells (Lucigen, Middleton, WI) to make the library.
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bioRxiv - Synthetic Biology 2022Quote: ... 2 uL of eluent was then electroporated into each of 2 tubes of 50 uL Endura electrocompetent cells (Lucigen, Cat#60242-2) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA extraction was carried out by lysing the microbes in fresh frozen tissue samples using Yeast Cell Lysis Buffer (Epicentre, Madison, WI, USA) and bead beating ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA from cell lines growing on 96-well plates was extracted by 25 µl QuickExtract DNA extraction solution (Epicentre #QE09050, Madison, WI, USA), further diluted to 200 µl with H2O ...
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bioRxiv - Microbiology 2021Quote: ... After the wash step cells were incubated in 200 ul PBS with 350u/ul of lysozyme solution (Epicentre ready-lyse, Epicentre Madison WI USA) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).