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Citations for Lucigen :
351 - 400 of 750 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
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bioRxiv - Cancer Biology 2021Quote: ... One of the plates was used for quick genomic DNA isolation for each of the colonies using QuickExtract DNA extraction solution (Lucigen # QE09050). 5μl of this lysate was used to set up a quick genomic PCR screening for IL-1β knockout clones using checking primers ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA from CD34+ cells or Bone Marrow of transplanted mice was extracted with the QuickExtract™ DNA Extraction Solution (Lucigen). For CD33 and each off-target site ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were then prepared from 5 ng of DNA by performing end-repair with the End-it DNA End-Repair Kit (Lucigen, ER81050), followed by A tailing with NEB Klenow Fragment (3’−5’ exo- ...
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bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
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bioRxiv - Genetics 2023Quote: ... at least 30 5-FOA resistant clones were grown in YEPD for DNA extraction by MasterPure™ Yeast DNA Purification Kit (Lucigen). The relative location of each chromosome truncation event was determined using multiplex PCR with primers that anneal centromere or telomere proximal to the SiRTA (File S1) ...
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bioRxiv - Genetics 2023Quote: ... Thirty clones that displayed growth on medium containing 5-FOA were selected and inoculated in liquid YEPD for genomic DNA extraction using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Multiplex PCR was used as described in Ngo et al ...
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bioRxiv - Developmental Biology 2023Quote: ... A piece of embryonic tail was taken from each embryo and DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from 2.5×105 Neuro2A cells with 250 μL of QuickExtract DNA Extraction Solution (Lucigen, Middleton, WI, USA) or from 30 mg of mouse liver using a EZ-10 Spin Column Animal Genomic DNA Miniprep Kit (Bio Basic ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from isolates that exhibited uracil auxotrophy was purified using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The promoter ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA for CRISPR/Cas9 genotyping was isolated from cell lines and organoids by using Quick Extract DNA Extraction Solution (Lucigen, #QE09050). For organoids ...
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bioRxiv - Biochemistry 2023Quote: ... coli (C41) cells for protein expression were purchased from Lucigen Corporation (Wisconsin) ...
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bioRxiv - Molecular Biology 2021Quote: ... whole genome sequencing was performed on DNA isolated from approximately 30 OD600 yeast cells using Lucigen MasterPure Yeast DNA Purification kit (Lucigen catalog: MMPY80200) according to the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was extracted after 48-72 hrs incubation using 50 µL Quick Extract™ DNA Extraction Solution (Lucigen, Middleton, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: The DNA isolation was performed on the swim up purified sperm using the MasterPure DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA) as previously described [13] ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reverse complement DNA templates were ordered from Integrated DNA Technologies (USA) and transcribed using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen). The DNase treated products were cleaned with Quick-RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Microbiology 2021Quote: ... and the phage pellet used for phage DNA preparation with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were selected and subcultured in THM to stationary phase and genomic DNA prepared using the Masterpure Gram-positive DNA purification kit (Lucigen, Middleton, WI). The samples then were purified further using ZymoDNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Cell Biology 2019Quote: ... it was extracted (QuickExtract DNA extraction solution, Epicentre Biotechnologies), the region of interest was amplified by PCR (primers described in Supplemental Table 4) ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using “Yeast Masterpure kit” (Epicentre, USA) following the manufacturer’s instructions.
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bioRxiv - Genetics 2020Quote: Total DNA was extracted using a QuickExtract kit (Lucigen). Unless otherwise indicated ...
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bioRxiv - Genetics 2020Quote: ... and a MasterPure™ Yeast DNA Purification Kit (Lucigen). PE150 libraries of the evolved strains were prepared and sequenced by Novogen.
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bioRxiv - Microbiology 2021Quote: ... swinhoei Y total DNA in the pWEB vector (Epicentre), was screened and a 3D growth-and-dilution library format as reported earlier45 ...
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bioRxiv - Genomics 2022Quote: ... 16 U of DNA Ampligase (Epicentre Technologies, Madison, WI), 0.016 mM dNTP ...
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bioRxiv - Genomics 2022Quote: ... 1x Ampligase DNA ligase buffer (Epicentre Technologies, Madison, WI), 0.5 μl of 5M Betain (Sigma Aldrich) ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted using the MasterPure kit (Lucigen, MC85200), and stored at 4C until use ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA for clone screens was extracted by QuickExtract (Lucigen) or ethanol precipitation.
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bioRxiv - Genetics 2020Quote: ... DNA was isolated using the QuickExtract kit (Epicentre, #QE09050) and the target region in the last exon of ACTN2 (Primers ...
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bioRxiv - Genetics 2020Quote: ... Worms were lysed in DNA Quick Lysate (Epicentre Technologies) for 1 hour at 60°C and the lysate was then used as a template for PCR with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... using Masterpure complete DNA and RNA purification kit (Lucigen), genomic DNA for whole genome sequencing was isolated ...
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bioRxiv - Neuroscience 2022Quote: 30 µl of Quick Extract DNA Estraction (Lucigen, QE09050,) was added to 5.0 x 104 pelleted iPSC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or the Epicentre MasterPure DNA Purification Kit (Epicentre Biotechnologies). All plasmid constructs were built with the Gibson Assembly Master Mix or the NEB Builder HiFi DNA Assembly (both New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... gDNA was extracted using the DNA QuickExtract Solution (Lucigen), followed by PCR and Sanger sequencing to determine efficient mutation rescue.
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bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
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bioRxiv - Genomics 2023Quote: ... 1µl Fast-Link DNA ligase (Lucigen, cat. no. LK6201H), 3 µl 10× Fast link ligation buffer and 1mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: QuickExtract DNA Extraction Solution (Lucigen, cat. No. LGN-QE09050)
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bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
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bioRxiv - Immunology 2021Quote: 200ul aliquots of blood samples collected on GD15 were used for genomic DNA (gDNA) extraction for bisulfite pyrosequencing using MasterPure DNA Purification kits (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Single cells were clonally expanded and genomic DNA was extracted from half the clonal population using QuickExtract™DNA Extraction Solution (Epicentre cat # QE09050) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Microbiology 2021Quote: ... two ml of a culture grown in R2B were used for extraction of high molecular weight (HMW) DNA using the MasterPureTM DNA Purification Kit (Epicentre, Madison, WI, USA), using the kit’s protocol for cell samples ...
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bioRxiv - Bioengineering 2022Quote: Samples collected at 24 h were processed for DNA isolation using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Protein purification was accomplished using the pETite N-His vector (Lucigen). PCR primers were designed to amplify products for BT2807 and BT2808 containing all amino acids downstream of the predicted signal peptide sequences ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were removed by precipitation with MCP solution (Lucigen, WI, USA) and the supernatant was collected after centrifugation at 17,000 x g for 10 min at 40C ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...