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Citations for Lucigen :
301 - 350 of 459 citations for 8 BROMO 4 METHYLTHIO 7 PHENYLPYRAZOLO 1 5 A 1 3 5 TRIAZIN 2 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... fragment 4 was subsequently subcloned into a low-copy plasmid pSMART LCAmp (Lucigen) to increase stability ...
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bioRxiv - Developmental Biology 2021Quote: Riboprobes and double-stranded RNA (dsRNA) for src-1 were generated by in vitro transcription (NxGen, Lucigen) as described previously (Petersen and Reddien ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and positive colonies were sequenced ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of purified RNA was used in cDNA synthesis with MMLV HP Reverse Transcriptase (Lucigen RT80125K) and 100 ng random hexamers (IDT) ...
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bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
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bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
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bioRxiv - Microbiology 2023Quote: ... The pBAC vector that can handle the full-length genome was purchased from Lucigen (cat # 42032-1). This vector was modified to include a CMV promoter ...
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Transcriptional dissection of symptomatic profiles across the brain of men and women with depressionbioRxiv - Neuroscience 2023Quote: ... RNA libraries were synthesized from 1 μg of RNA using the ScriptSeq Complete Gold Kit (Epicentre, Illumina) including an initial ribosomal RNA depletion step ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (Catalogue Number: 14000-1, Lucigen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... NxSeq DNA sample prep kit 2 (Lucigen, Middleton, WI, USA) was used as per manufacturer’s directions with either NEXTFlex 48 barcodes (BioO ...
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bioRxiv - Microbiology 2019Quote: ... and electroporated with the Ez-Tn5
2> transposome (Epicentre). Cells were recovered for 90 minutes at 30 °C with shaking ... -
bioRxiv - Neuroscience 2021Quote: ... and NxGen RNase Inhibitor (0.2 U/μl; Lucigen, 30281-2). Tissue was then triturated by fire-polished Pasteur pipette (three pipettes of decreasing diameter ...
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bioRxiv - Microbiology 2021Quote: The EZ-Tn5
2> Tnp transposome (Epicentre Biotechnologies, U.S.A) was introduced into E ... -
bioRxiv - Synthetic Biology 2022Quote: ... coli 10G electrocompetent cells (60080-2, Lucigen, Middleton, WI, US). Cells were selected with appropriate antibiotics on solid and liquid culture ...
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bioRxiv - Cancer Biology 2022Quote: ... and CAGs-rtTA3 PCRs using EconoTaq PLUS (Lucigen #30033-2). Doxycycline chow (food pellets ...
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bioRxiv - Cell Biology 2023Quote: ... followed by electroporation into Endura ElectroCompetent Cells (Lucigen, 60242-2) at ∼100x coverage ...
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bioRxiv - Cell Biology 2023Quote: ... TEV protease 96 or SUMO Express proteinase (Lucigen, 30801-2) was respectively incubated with GST-TEV-tagged G3BP1 proteins and His-SUMO-tagged N proteins at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and 60 ug total RNA per sample was incubated with 3 uL RNase I (Epicentre #N6901K) for 45 minutes at RT with light shaking ...
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bioRxiv - Synthetic Biology 2020Quote: Transposon Cassettes were inserted into pNL4-3 by in vitro transposition with EZ-Tn5 transposase (Epicentre) per manufacturer’s protocol and with equal mols of plasmid template and transposon ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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bioRxiv - Genomics 2019Quote: ... The sample was placed on ice and preincubated with the CircLigase II reaction buffer on ice for 5 min (Nuclease-free H2O, 1 × CircLigase buffer, 2.5 mM MnCl2, 0.5 M Betaine, [all included in kit from CL9025K, Epicentre]). After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomal RNA was depleted from 1 µg of total RNA using the Ribo-Zero rRNA Removal Kit (Epicentre) for Plants and Bacteria ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were lysed with 1 ml mammalian lysis buffer containing 200 µl 5x Mammalian Polysome Buffer (Epicentre, RPHMR12126), 100 µl 10% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg RNA was used for reverse transcription with 0.5 μl MMLV HP reverse transcriptase (Lucigen cat # RT80125K) per 20 μl rxn ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA (1 μg) from untreated HPAECs was digested by 2U of RNase R enzyme (Lucigen, Middleton, WI) in 10 μL reactions containing 1x digestion buffer ...
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bioRxiv - Cell Biology 2021Quote: ... This sequence was cloned into the pSmart (Lucigen Cat#40041-2) shuttle vector using Gibson assembly (New England Biolabs) ...
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bioRxiv - Biophysics 2023Quote: ... Genomic DNA was extracted 3 days post-transfection using QuickExtract DNA Extraction Solution 1.0 (Lucigen Corporation QE09050). To test the cutting efficiency ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... then digested with exonuclease I by adding 1 μL of enzyme (20 U/μL) plus 1.5 μL 10X Reaction Buffer (Lucigen) and incubating 1 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: Stranded RNA-seq libraries were prepared from 1 μg total RNA using ScriptSeq v2 Complete kit for bacteria (Epicentre) and sequenced on Illumina NovaSeq 6000 platform at GENEWIZ ...
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bioRxiv - Systems Biology 2020Quote: ... RNA digestion of lysates was performed for 1 hour with the mixture of 2000 Units of RNase T1 (Epicentre) and 300 Units of RNase S7 (Roche/Sigma) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a rolling-circle amplification (RCA) reaction mixture (1 unit/μL phi29 polymerase (Lucigen, NxGen kit 30221), 1x phi29 polymerase buffer ...
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bioRxiv - Genetics 2023Quote: ... and plasmid replication was induced by diluting 1:10 in LB + 12.5 µl/ml chloramphenicol and 1X CopyControl Induction Solution (Lucigen). The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
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bioRxiv - Cell Biology 2022Quote: ... Isolated genomic DNA was combined with EconoTaq PLUS GREEN 2X Master Mix (cat# 30033-1; Lucigen, Middleton, WI, USA) and forward and reverse primers (primer information listed in Table S2 ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 8-well strips containing 3 μL of cell collection buffer (0.1% Triton X-100, 0.2 U/μL RNAse inhibitor (Lucigen)) ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Excess linker was degraded by adding 2 μl 10× RecJ buffer (Lucigen), 1 μl RecJ exonuclease (Lucigen) ...
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bioRxiv - Microbiology 2019Quote: ... subvibrioides ΔdivK mutant using the EZ-Tn5
2> TNP transposome (Epicentre). B ... -
bioRxiv - Genetics 2019Quote: ... 2 ul of Hybridase Thermostable RNase H (Epicenter, Madison, WI: Lucigen H39500) to make 10 ul total and incubated for 30 minutes at 45°C ...
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bioRxiv - Cell Biology 2023Quote: ... Library (50ng) was electroporated into 25µL Endura electrocompetent cells (60242-2, Lucigen). Cells from eight electroporations were pooled and rescued in 8mL of rescue media for 1hr at 37 ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of cells were mixed with EZ-Tn5TM
2> transposome (Lucigen) and incubated on ice for 30 min ... -
bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre Cat. # QE09050) to the cells ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted by adding 4 volumes of QuickExtract DNA Extraction Solution (Epicentre, Cat # QE09050) to the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... blunt-end ligation into the pZE21 MCS1 expression vector with constitutive promoter pLteo-1 (Lutz and Bujard, 1997) at the HincII site using the Fast Link ligation kit (Epicentre), and iv ...
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bioRxiv - Immunology 2019Quote: ... washed once in PBS and genomic DNA was recovered from 1 x 106 cells using 100 μl Quick Extract solution (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Subsequent library preparation was performed with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen®; Cat. no. 14000-1) according to the manufacturer’s instructions with one slight modification ...
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bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in Tris-EDTA (TE) buffer (10 mM Tris-Cl, pH 8.0, 1 mM EDTA) containing lysozyme (Epicentre; R1804M) and 1% SDS ...
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bioRxiv - Microbiology 2021Quote: ... flhD or complexed AsflhD with flhD was performed at 37°C in TMN buffer containing 1 µg tRNA for 15 min with RNase III (Epicentre). Samples were loaded on denaturing polyacrylamide gels together with an RNA alkaline ladder as in (75).
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...