Labshake search
Citations for Lucigen :
251 - 300 of 426 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). Primers specific to the blasticidin resistance cassette and IRAK1/IRAK4 gene loci were used to verify the insertion (see Table S5 and S6 for IRAK4 and IRAK1 respectively) ...
-
bioRxiv - Immunology 2020Quote: ... DNA was isolated from the clones using the QuickExtract DNA Extraction Solution (Lucigen, Middleton, USA). Upon amplification and sequencing (Eurofins Genomics ...
-
bioRxiv - Genetics 2020Quote: DNA was isolated from each of the four cell pools with 100 µL QuickExtract (Lucigen) per 1 million cells following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was extracted from the clones by cell lysis with Quickextract DNA extraction solution (Lucigen), and used as template for PCR with 2x Hotstart PCR mix (Qiagen ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
-
bioRxiv - Immunology 2023Quote: ... Ribosomal RNA was depleted from 10μg total RNA using the Ribo-Zero Magnetic Kit (Epicentre). Ribo-depleted RNA was fragmented ...
-
bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from selected monoclonal cell lines using QuickExtract DNA Extraction Solution (Epicentre). To test for gene editing and correct insertion of mGFP/mScarlet-i cassette ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from single cell clones using QuickExtract DNA Extraction Solution 1.0 (Lucigen) and the region of interest was amplified by two consecutive PCRs ...
-
bioRxiv - Neuroscience 2024Quote: Genomic DNA was extracted from iPSCs and NPCs using QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
-
bioRxiv - Synthetic Biology 2020Quote: Golden gate assembly reactions were transformed into chemically competent Escherichia coli prepared from strain TG1 (Lucigen). Transformed cells were selected on Lysogeny Broth containing the antibiotics chloramphenicol ...
-
bioRxiv - Microbiology 2021Quote: Ribosomal RNA was removed from strain B50 transcriptomes by using the Ribo-Zero Magnetic Kit (EPICENTRE Biotechnologies ...
-
bioRxiv - Biophysics 2020Quote: Genomic DNA was isolated from candidate cell lines using the QuickExtract DNA Extraction Solution (Lucigen, #QE09050) essentially as described ...
-
bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from bacterial cell pellets using MasterPure™ complete DNA purification kit (Lucigen). Oxford Nanopore Minion Sequencing ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... DNA was isolated from PHH using a MasterPure Complete DNA Purification kit (Epicentre, Madison, WI; MC85200) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on an Illumina HiSeq2500 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on the Illumina HiSeq2500 platform (100 bp single-end reads) ...
-
bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from sorted and non-sorted cells using the QuickExtract reagent (Lucigen QE09050). In addition ...
-
bioRxiv - Genomics 2021Quote: ... DNA was extracted from whole adult worms using the MasterPure™ DNA Purification kit (Epicentre Biotechnologies) at the Department of Veterinary Disease Biology (VDB) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total RNA was then treated for 15min at 37°C with 10U RNase R from Epicentre in a 20 μl reaction volume ...
-
bioRxiv - Neuroscience 2023Quote: ... The ribosomal RNA was removed from the purified RNAs using the Ribo-Zero magnetic kit (Epicentre). The Ribo-seq library was constructed using the ARTseq™ Ribosome Profiling Kit (Epicentre) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Library preparation was performed with ribosomal RNA (rRNA) depletion according to instructions from the manufacturer (Epicentre) to achieve greater coverage of mRNA and other long non-coding transcripts ...
-
bioRxiv - Immunology 2023Quote: ... The pDNA for IVT were isolated from the E.coli BigEasy™-TSA™ Electrocompetent Cells (Lucigen) using the Plasmid Maxi Kit (QIAGEN) ...
-
bioRxiv - Immunology 2022Quote: ... DNA extract was prepared from an aliquot of cells with QuickExtract DNA Extraction Solution (Lucigen Corporation). Only wells containing single clones were screened using PCR primers flanking the Cas9 cut sites and by Sanger sequencing (Genewiz ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was poly-adenylated by Poly(A) Polymerase Tailing Kit (Epicentre) and depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen) ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted from the homogenized sample using the MasterPure Complete DNA & RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... Ribosomal RNA (rRNA) was depleted from each RNA sample using the Ribo-ZerorRNA Removal Kit (Epicentre), designed for human ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were harvested and DNA was extracted from cells using QuickExtract DNA Extraction Solution (Lucigen Corporation). The pellet was dissolved in 20-80 uL of QuickExtract solution ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from triplicate co-cultures with the MasterPure complete DNA purification kit (Lucigen). The proportion of G ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µL of Ready-Lyse Lysozyme Solution from a MasterPure Gram Positive DNA Purification Kit (Epicentre), 45 units mutanolysin ...
-
bioRxiv - Genetics 2022Quote: DNA was extracted from whole blood following the MasterPure™ DNA Purification kit (Epicentre, MCD 85201). DNA passing quality control based on NanoDrop spectrometry and in sufficient amount through the Qubit dsDNA Broad Range Assay Kit (ThermoFischer Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... DNA extraction from various hiPSC clones was carried out using the QuickExtract DNA Extract Solution (Epicentre), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Bacterial DNA was extracted from each CFU recovered using the QuickExtract DNA extraction solution (Epicentre, # QE09050) and each colony was screened for the presence of tbpA and lbpA genes ...
-
bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. All samples were treated with Invitrogen TURBO DNA-Free kit to remove DNA contamination ...
-
bioRxiv - Molecular Biology 2024Quote: ... 40-60 μg of RNA from the lysate were treated with RNAse I (Lucigen, Cat. #N6901K) for 50 minutes at room temperature while rotating ...
-
bioRxiv - Developmental Biology 2024Quote: ... Genomic DNA was extracted from individual samples using 40 µL QuickExtract DNA extraction solution (Lucigen QE0905T). Embryos were incubated for 15 min at 25 °C ...
-
bioRxiv - Genetics 2021Quote: ... rRNA was depleted from the purified nsRNA fraction using Terminator™ 5′-Phosphate-Dependent Exonuclease (Lucigen TER51020) as per the manufacturer’s protocol ...
-
bioRxiv - Genetics 2021Quote: ... genomic DNA from bulk cells of each transfected well was extracted using Quick Extract buffer (Lucigen #QE0905T). following the protocol published by the manufacturer (https://www.lucigen.com/docs/manuals/MA150E-QuickExtract-DNA-Solution.pdf) ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and positive colonies were sequenced ...
-
bioRxiv - Cell Biology 2022Quote: ... The homology arms were amplified from extracted HEK-293 cell genomic DNA (QuickExtract DNA extraction solution, Lucigen). The fidelity of all constructs was confirmed by Sanger sequencing prior to use.
-
bioRxiv - Microbiology 2022Quote: ... DNA was isolated from overnight cultures using a MasterPure™ Gram Positive DNA Purification Kit (Epicentre, USA). Nanopore libraries were prepared using Ligation Sequencing Kit (SQK-RBK109 ...
-
bioRxiv - Microbiology 2022Quote: DNA was extracted from 154 clinical and environmental samples using the MasterPure™ DNA Purification Kit (Epicentre), following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5ug of RNA from individual tissues were treated with 15 units of RNAse R (Epicentre, Illumina, USA) for 15 minutes at 37 degrees Celsius ...
-
bioRxiv - Microbiology 2023Quote: ... The pBAC vector that can handle the full-length genome was purchased from Lucigen (cat # 42032-1). This vector was modified to include a CMV promoter ...
-
bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
-
bioRxiv - Microbiology 2023Quote: ... rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies, United States) and the mRNA was chemically fragmented to short pieces (200 nt ...
-
bioRxiv - Developmental Biology 2022Quote: Tissue samples (ear and tail clips) from resulting animals were lysed using QuickExtract DNA Extraction Solution (Epicentre) followed by PCR at the specific genomic location ...
-
bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from concentrated cells using the MasterPure complete DNA and RNA purification kit (Epicentre) and sequenced using P6 chemistry on a RS II instrument (Pacific Biosystems) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and genomic DNA was extracted from them using the MasterPure DNA Purification kit (Epicentre, Madison, WI, USA). Genomic DNA from one additional human iPSC (AG25370 ...