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Citations for Lucigen :
151 - 167 of 167 citations for RNase Detection since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Neuroscience 2022Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution (corresponding to 10 µg of total RNA) was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 minutes at a temperature of 25°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Neuroscience 2019Quote: ... The samples were then pelleted at 500rcf for 10 min at 4C followed by a wash in 10mL of Nuclei Wash & Resuspension Buffer (1X PBS, 1% BSA, 0.2U/ul NxGen RNase inhibitor (Lucigen, 30281-2)) ...
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bioRxiv - Genetics 2019Quote: ... slowly reduced the temperature to 65°C and then added 2U of Hybridase Thermostable RNase H (Epicenter, Madison, WI: Lucigen H39500) and 1 ul of 10X Digestion buffer (500 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was then incubated at 37 °C for 1 h with 20 U of RNase R (Lucigen, cat n°RNR07250), to remove all the undesired products (i.e linear RNA or concatemer product) ...
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Planarians employ diverse and dynamic stem cell microenvironments to support whole-body regenerationbioRxiv - Developmental Biology 2023Quote: ... pucks with tissue adhered were immediately immersed in 200 μL of hybridization buffer (6X SSC, 2 unit/μL Lucigen RNase inhibitor) for 27 minutes at room temperature to facilitate the binding of mRNA to the spatially barcoded beads of the puck ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...
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bioRxiv - Neuroscience 2024Quote: ... we pooled all mice into the same tube containing a hypertonic lysis buffer (10mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal, 0.2 U/μl Lucigen NxGen Rnase inhibitor). Nuclei were extracted using a glass dounce homogenizer (DWK ...
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bioRxiv - Developmental Biology 2019Quote: ... and was then incubated for 30 min at 37 °C with or without 5 U μg −1 of RNase R (Epicentre Bio-technologies). Reverse transcription was performed using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Microbiology 2024Quote: ... and approximately 80 µg of RNA in 1 mL volume was digested with 5 U of RNase I (Lucigen Cat#E0067-10D1) for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Comparable results to commercial RNA extraction kits have been obtained using a 5-min direct detection preparation method of nasopharyngeal samples following 1:1 dilution with the Quick Extract DNA extraction Solution (Lucigen) (6) ...
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bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
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bioRxiv - Microbiology 2023Quote: ... 1mL of cell culture was collected and used for genome DNA extraction using Lucigen MasterPure Complete DNA & RNA with additional RNase step (Lucigen, Mandel Scientific, Guelph, ON, Canada) following the manufactures’ manual.