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Citations for Lucigen :
151 - 200 of 747 citations for Cow Glucosidase 2 Subunit Beta PRKCSH ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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bioRxiv - Genomics 2021Quote: ... Non circularized templates were eliminated by 2 h digestion at 37°C with Plasmid-safe DNAse (Epicentre, #E3101K), the enzyme was inactivated by heating for 30 minutes at 70°C ...
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bioRxiv - Microbiology 2021Quote: ... coli chromosome was carried out by electroporating 1 μL of the EZ-Tn5
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bioRxiv - Neuroscience 2021Quote: ... we electroporated 1 μL of 25 ng/μL of each library into 50 μL bacteria (Lucigen, 60242-2), with ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Clones were identified ∼2 weeks after sorting and screened by genomic DNA extraction with Quickextract (Lucigen cat.#QE09050) and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712) ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the reaction was used to transform 25 μl of Endura electrocompetent cells (Lucigen; 60242-2) according to the manufacturer’s protocol using a Gene Pulser (BioRad) ...
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bioRxiv - Genetics 2023Quote: ... the Gibson reaction products were purified by isopropanol precipitation and electroporated into Endura electrocompetent cells (Lucigen, 60242-2). The transformed bacteria were spread onto LB agar plates containing 100 µg/mL ampicillin and incubated overnight at 37℃ ...
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bioRxiv - Genomics 2022Quote: Shot-gun library was generated at the McGill Genome Center using the NxSeq® AmpFREE Low DNA Library Kit Library Preparation Kit (Lucigen Corp., WI, USA) according to the manufacturer’s recommendations and the library was ran on a HiSeq X for 2×150 cycles.
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were produced using the Art-Seq kit (Epicentre/Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... with the Ribo-Zero rRNA removal kit for Bacteria (Epicentre) according to standard protocol ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen), and treated with TURBO DNase (ThermoFischer ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA was depleted using Ribo-ZERO magnetic kit (Epicentre). The transcripts were fragmented and used as templates to generate strand-specific cDNA libraries by TruSeq Stranded Total RNA LT Sample Prep kit (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to make the libraries according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... genomic DNA was extracted with the QuickExtract kit (Epicentre, #QE09050). PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix ...
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bioRxiv - Microbiology 2021Quote: The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to prepare directional multiplex libraries ...
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bioRxiv - Molecular Biology 2020Quote: ... and processing with a RiboZero rRNA removal kit (Epicentre/Illumina) to deplete rRNA ...
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bioRxiv - Genomics 2021Quote: DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit and sequencing was performed on the Illumina NextSeq500 with 75bp single-end reads (first experiment) ...
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bioRxiv - Microbiology 2020Quote: ... rRNA was removed using a Ribo-Zero (Bacteria) kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... ScriptSeq v2 RNA-Seq library preparation kit (Epicentre Technologies, USA), TruSeq SBS Kit v3-HS (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted with Master Pure purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells using the MasterPure Yeast RNA Purification Kit (Epicentre). The procedure for removal of DNA contamination was as per the specification of the manufacturer but performed twice to efficiently remove the DNA from the samples ...
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bioRxiv - Neuroscience 2022Quote: ... were ligated to the ChIP DNA (Fast-Link kit; Epicentre) and PCR amplified using the Illumina TruSeq ChIP Library Prep Kit ...
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bioRxiv - Microbiology 2022Quote: ... depleted in ribosomal RNA (Ribo-Zero rRNA Removal Kit, Epicentre) and reverse transcribed into cDNA ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA (rRNA) was depleted (Ribo-ZeroTM Gold Kit, Epicentre), cDNA libraries were built with TrueSeq total RNA sample kit (Illumina ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit ...
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bioRxiv - Genomics 2024Quote: ... using the NxSeq® AmpFREE Low DNA Library Kit (Lucigen) and sequenced to obtain paired-end short reads (150 bp) ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was circularized by using the CircLigaseII Kit (Lucigen, CL9021K). The samples were subjected to PCR to introduce Illumina i7 indexes ...
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bioRxiv - Developmental Biology 2024Quote: ... and tailed with a poly(A) polymerase tailing kit (Epicentre) and purified with RNA clean & concentrator-25 kit (Zymo Research).
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bioRxiv - Genomics 2024Quote: ... The NxSeq AmpFREE Low DNA Library Kit (Lucigen - CA10837-144) was then used ...
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bioRxiv - Neuroscience 2022Quote: ... The puck was immersed in 200 µL of hybridization buffer (6X SSC, 2 unit / µL Lucigen NxGen RNAse inhibitor) for 30 minutes at 37°C for the binding of RNA to the oligos ...
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bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.
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bioRxiv - Microbiology 2022Quote: Positive fosmid clones were cultivated in 100 mL LB medium containing 12.5 μg/ml chloramphenicol and 2 ml/L fosmid autoinduction solution (Epicentre) at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: Step 2 - extension & ligation: 10 μl of pre-heated (60°C) extension/ligation mix containing 5U of ampligase (Lucigen), 4U of Phusion HF DNA polymerase (#F530S ...
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bioRxiv - Systems Biology 2023Quote: ... Eight transformation reactions were conducted with 2 μl of ligation product into each tube of electrocompetent cells (Lucigen, #60242) and plated onto eight 15-cm plates with Carbenicillin selection (50 μg/ml) ...
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bioRxiv - Genomics 2024Quote: ... Incorporation of the fluorophore into dsDNA was done through a primer extension reaction using EconoTaq polymerase (Lucigen, 30035–2) and purified using the QIAquick PCR purification kit (Qiagen 28106) ...
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bioRxiv - Genetics 2024Quote: ... The supernatant was discarded and 1.5mL Buffer D (0.01% BSA in 1X PBS with 0.5U/uL RNase inhibitor [Lucigen 30281-2]) was gently added to the nuclei pellet and incubated on ice 20min ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Molecular Biology 2020Quote: Rolling Cycle Amplification (RCA) was done O/N at 30°C using 0.5 Units/μl Φ29 polymerase (Lucigen, 30221-2). The reaction mixture contained also 1X Φ29 buffer ...
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bioRxiv - Bioengineering 2022Quote: ... at 37°C (1 – 2 h) and 0.9 μl from the reaction mix were electroporated to E.coli Cloni® 10G cells (Lucigen, USA). Colony PCR and sequencing were used for analysis and verification.
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bioRxiv - Genomics 2021Quote: ... 1 μl of the Gibson reaction was delivered to 25 μl of electrocompetent EnduraTM cells (Lucigen, cat. no. 60242-2) using Gene Pulser®/MicroPulser™ Electroporation Cuvettes ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor ...
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bioRxiv - Immunology 2021Quote: Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells from each of these 4 wells were dissociated and divided into 2 portions–one portion was used to collect genomic DNA using QuickExtract DNA Extraction solution (Lucigen) while 500 cells from the other portion was suspended in 25 ml of culture medium and 100 ul was seeded into each well of two 96-well plates for further culture ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...