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Citations for Lucigen :
101 - 150 of 155 citations for Palmitic acid U 13C16 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... We treated cell lysate containing 8.5 µg total RNA with 20 U RNase I (Epicentre) in a 300 µl reaction at 25°C for 45 min ...
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bioRxiv - Genetics 2023Quote: ... 400 ng of DNA was incubated with 5 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Molecular Biology 2024Quote: ... UHRR and HeLa S3 RNAs (1 μg) were incubated with 20 U exonuclease I (Lucigen) and 2 U Baseline-ZERO DNase (Lucigen ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation with 1 U/μl NxGen Phi29 DNA polymerase (Lucigen, cat# 302210-2), 250 μM dNTPs ...
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bioRxiv - Genomics 2020Quote: ... 2.5 μL of T4 polynucleotide kinase (5 U/μL) plus 2.5 μL 10X Reaction Buffer (Lucigen) were added to the initial 20-μL nucleic acids solution ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... and excess unligated linker was removed using 10 U/µl 5’ deadenylase/RecJ exonuclease (Epicentre, RJ411250), followed by pooling and purification of ligated footprints using an Oligo Clean and Concentrator column (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates containing 3 μg of total RNA were treated with 20 U of RNase I (Lucigen) for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted using the Epicentre MasterPure nucleic acid extraction kit (Epicentre, Madison, WI) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... recovered and used for the 20 µL circularization reaction (100 U CircLigase™ ssDNA Ligase (Lucigen, CL4111K), 50 mM ATP ...
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bioRxiv - Molecular Biology 2022Quote: ... the lysates containing 10 µg of total RNA were treated with 2 U/µg RNase I (Lucigen) at 25°C for 45 min ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the Sterivex filters using the Masterpure™ Nucleic Acid Extraction Kit (Epicentre). Six-hundred microliters of Masterpure™ Tissue and Cell Lysis Solution containing recommended quantities of proteinase K were added to each Sterivex filter ...
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bioRxiv - Cell Biology 2021Quote: ... Fungal genomic acid DNA was isolated with the MasterPureTM Yeast DNA Purification Kit (Lucigen, Wisconsin, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... two µg of total RNA were treated with 5 U of RNase R (cat. # RNR07250, Lucigen, Middleton, WI) for 40 min at 37 °C ...
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bioRxiv - Genomics 2022Quote: Equal amounts of RNA (2µg) were incubated with or without 20 U of Rnase R (RNR07250, Epicentre Biotechnologies) and 40 U of RiboLock RNase inhibitor (EO0381,Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... placed into 8-well strips containing 3 μL of cell collection buffer (0.1% Triton X-100, 0.2 U/μL RNAse inhibitor (Lucigen)) ...
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bioRxiv - Genetics 2023Quote: ... 15-20 μg of genomic DNA (gDNA) was digested with ∼95 U Plasmid-Safe ATP-Dependent DNase (Lucigen), 25 mM ATP solution ...
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bioRxiv - Systems Biology 2023Quote: ... Lysate containing 20 µg of total RNA was digested with 10 U of RNase I (Lucigen, WI, USA) for 45 min at 25°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 min at 25°C ...
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bioRxiv - Genomics 2024Quote: ... reactions were supplemented with 10 µL of RNase H mix (10 U Hybridase Thermostable RNase H (Lucigen; H39500), 20 mM MgCl2 ...
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bioRxiv - Genomics 2020Quote: ... then digested with exonuclease I by adding 1 μL of enzyme (20 U/μL) plus 1.5 μL 10X Reaction Buffer (Lucigen) and incubating 1 h at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... 100μg total RNA from cortex or cultured HEK293 cells was treated with or without 1μL of RNaseR [20 U/μl] (Lucigen), plus 1.9μL RNaseOUT [40 U/μL] (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The size-selected RNAs or synthetic versions of FLEXIs were subjected to exonuclease treatment (1 U Terminator exonuclease (Lucigen), 20 U RNase R (Abcam) ...
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bioRxiv - Cell Biology 2024Quote: RNAs from cells (2,000 ng) and EVs (960 ng) was incubated with 1 U/µg RNase R (Lucigen, RNR07250) at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
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bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... from lysate of the filamentous bacterial fraction acquired by incubation for 8 hours with 10 U/µl Ready-lyse lysozyme (Epicentre). The sequencing library was prepared using the Nextera XT kit and sequenced the same way as for the SAGs.
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 290 µl of the lysate solution (corresponding to 10 µg of total RNA) was incubated with 10 µl of 10 U/µl RNase I (Lucigen) for 45 minutes at a temperature of 25°C ...
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bioRxiv - Microbiology 2023Quote: ... then resuspend in 1mL resuspension buffer (20 mM Tris-HCl pH 7.5, 300 mM NaCl, 12500 U Ready-lyse (Epicentre technologies), and one tablet of EDTA-free protease inhibitor cocktail) ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ∼4 pmol of the purified ligated product obtained from an overnight ligation reaction between CS3 and PPP-Lig was digested with 20 U of RNase R (Lucigen) in a 10 μL reaction in the presence of 1X RNase R buffer at 50 °C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Obtained cDNA was circularized at 60°C overnight in a 20 μL reaction mixture consisting of 10 U with CircLigase ssDNA ligase (Lucigen), 1x reaction buffer (Lucigen) ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was removed and cells were resuspended in 7 mL PBS-RI (1x PBS supplemented with 0.1 U/µL NxGen RNase inhibitor (Lucigen, CN 30281)) ...
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bioRxiv - Cell Biology 2021Quote: Total RNAs was treated with RNase R for 30 min at 37°C using 3 U/mg of RNase R (Lucigen, USA). HCC-LM3 and Huh-7 cells treated with actinomycin D (1 µg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was then incubated at 37 °C for 1 h with 20 U of RNase R (Lucigen, cat n°RNR07250), to remove all the undesired products (i.e linear RNA or concatemer product) ...
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bioRxiv - Microbiology 2023Quote: ... at 2 tabs per 50 mL and 100 U of Ready-lyse/ml of culture (Ready-Lyse Lysozyme from Epicentre Lucigen) prior 10 minutes of incubation at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... we pooled all mice into the same tube containing a hypertonic lysis buffer (10mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal, 0.2 U/μl Lucigen NxGen Rnase inhibitor). Nuclei were extracted using a glass dounce homogenizer (DWK ...
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bioRxiv - Molecular Biology 2024Quote: ... the recovered cDNA was circularized by incubation in a reaction volume of 10 μl in the presence of 50 U CircLigase™ ssDNA Ligase (Epicentre), 1 M betaine ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl) and resuspended in 300 µL of buffer A supplemented with 100 U/mL Ready-Lyse Lysozyme (Epicentre Lucigen), 10 µg/mL of DNAse I (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... we performed sucrose cushion to pellet the ribosome-associated mRNAs and proceeded with RNAse I digestion (10 U/μl by the Epicentre definition) for 30ug of RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Microbiology 2021Quote: ... The ssDNA linker containing a 5′ phosphate and 3′ C3 spacer was ligated to the synthesized cDNA using 20 U of the Circligase I (Lucigen, Middleton, WI). The resultant cDNA was amplified by an adapter-based PCR using the KAPA HiFi DNA polymerase (Roche ...