Labshake search
Citations for Lucigen :
101 - 131 of 131 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
-
bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... A minimum of 1.2ug of ligated pUSEPR plasmid DNA per sub-pool was electroporated into Endura electrocompetent cells (Lucigen), recovered for one hour at 37°C ...
-
bioRxiv - Genomics 2022Quote: ... A total of 1.2 µg of ligated pUSEBR-CRISPR Library plasmid DNA was then electroporated into Endura electrocompetent cells (Lucigen). Competent cells were recovered for 1 h at 37ºC ...
-
bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.
-
bioRxiv - Genomics 2021Quote: ... adding additional ATP and DNase every 24 h (30 units per day) according to the manufacturer’s protocol (E3101K, Plasmid-Safe ATP-dependent DNase, Epicentre,). ecDNA-enriched samples were used as template for phi29 polymerase amplification reactions (150043 ...
-
bioRxiv - Microbiology 2022Quote: ... T7 promoter (TAATACGACTCACTATAG) followed by the first 1629 nucleotides of the VA1 genome inserted into the pSMART plasmid (Lucigen). A second plasmid ...
-
bioRxiv - Genomics 2023Quote: ... The resulting plasmid libraries were purified and concentrated via SPRI bead cleanup before being transformed into electrocompetent cells (Lucigen Endura ...
-
bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... pre-digested DNA was extracted and further incubated with 20 U plasmid-safe DNase (PS-DNase, Lucigen®, Epicentre, UK) and 1mM ATP at 37 °C for 24 h ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli strains DLF_R002 and DLF_R003 were constructed as previously described.10 Reporter plasmids were constructed in the pSMART-HC-Kan (Lucigen, WI) backbone ...
-
bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
-
bioRxiv - Cancer Biology 2022Quote: ... The oligo pools were cloned into a modified pLKO-TET-ON plasmid by the Golden Gate method and the resulting product was used to transform Endura electrocompetent cells (Lucigen) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from bulk cells transfected with CRISPR/Cas9 plasmids using the epicentre QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2023Quote: ... Fragments from 500 bp to 1000 bp were excised from an agarose gel and cloned into the pSMART LCKan plasmid (Lucigen) by transforming into competent cells ...
-
Microhomology-Mediated Circular DNA Formation from Oligonucleosomal Fragments During SpermatogenesisbioRxiv - Genomics 2023Quote: ... during which additional ATP and DNase was added every 24LJh (30 units per day) according to the manufacturer’s protocol (Plasmid-Safe ATP-dependent DNase, Lucigen, E3101K). The eccDNA samples were cleaned by phenol ...
-
bioRxiv - Molecular Biology 2019Quote: One microgram of total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre, Madison, WI, USA) followed by a purification step using AMPure XP Beads (Beckman Coulter ...
-
bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
-
bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
-
bioRxiv - Microbiology 2021Quote: ... OmRV-fragment2/pMA (ampicillin resistant) and pACYC177 low-copy-number plasmids were transformed into 10G chemically competent cells (Lucigen, E. cloni), respectively ...
-
bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
-
bioRxiv - Zoology 2023Quote: ... two micrograms of the pooled shrimp genomic DNA was aliquoted and subjected to linear DNA digestion using plasmid-safe DNase (Epicentre, UK) for 4 days ...
-
bioRxiv - Neuroscience 2021Quote: ... in the pET-28 bacterial expression vector together with pLysSRARE2 plasmid (both kindly shared by Dr. Chernomordik, NICHD) were electroporated into ClearColi™ BL21(DE3) according to manufacturer protocol (Lucigen, WI). Bacterial colonies that grew in an LB plate containing 100 μg/ml kanamycin and 30 μg/ml chloramphenicol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
-
bioRxiv - Synthetic Biology 2022Quote: ... two ncRNA expression cassettes (for barcoded ncRNAs “A” and “B”) from the Marionette plasmids were cloned into pSol-TSF (Lucigen F843213-1) facing in opposite directions ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 22 Alanine production plasmids were constructed using codon optimized (Codon Optimization Tool from the IDT) synthetic DNA and the pSMART-HCKan vector (Lucigen, Middleton, WI). Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB ...
-
bioRxiv - Molecular Biology 2021Quote: ... Extraction of circular DNA from total DNA was performed by enzymatic digestion of linear DNA using the plasmid-safe DNase (PS-DNase) (Lucigen®, Epicentre, UK) that “digests linear dsDNA and ...
-
bioRxiv - Microbiology 2023Quote: ... Digested fragments were cloned into the BamHI/NcoI pre-digested pET15b plasmid using the Fast-Link™ DNA Ligation Kit (Epicentre, Madison, WI), and E ...
-
bioRxiv - Molecular Biology 2022Quote: ... Complete cDNAs were sequence-amplified by PCR using KOD One™ PCR Master Mix -Blue- (TOYOBO, Japan) and were cloned into pSMART-LCK plasmid (Lucigen, Middleton, WI, USA) containing a T7 RNA polymerase promoter ...