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Citations for Lucigen :
51 - 100 of 844 citations for Endothelin 1 ET 1 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 1 x Ampligase® Reaction Buffer (Lucigen), 10 U Ampligase (Lucigen) ...
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bioRxiv - Genomics 2022Quote: ... 1 U/μL RNase Inhibitor (NxGen, Lucigen), 1 U/μL reverse transcriptase (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... in 1× RNase R buffer (#RNR07250; Epicentre) in a 10 µl reaction volume at 37 °C for 10 min followed by heat inactivation at 95 °C for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1 U/µL Nxgen RNase inhibitor (Lucigen), and 0.1% Tween 20 (blocking buffer ...
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bioRxiv - Genomics 2019Quote: ... The sample was placed on ice and preincubated with the CircLigase II reaction buffer on ice for 5 min (Nuclease-free H2O, 1 × CircLigase buffer, 2.5 mM MnCl2, 0.5 M Betaine, [all included in kit from CL9025K, Epicentre]). After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomal RNA was depleted from 1 µg of total RNA using the Ribo-Zero rRNA Removal Kit (Epicentre) for Plants and Bacteria ...
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bioRxiv - Biochemistry 2020Quote: ... ratio overnight at 16 °C followed by purification and then electroporation into phage-display competent TG-1 cells (#60502-1, Lucigen). The library was plated onto 2xYT agar plates containing 100 µg/mL carbenicillin and 2% glucose at 37 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... and aliquots of 100 μL were dispensed into 0.1 mm gapped electroporation cuvettes along with 1 μg of plasmid DNA and 1 μL of Type-One restriction inhibitor (Epicentre). Electroporation was performed with a Bio-Rad Micropulser (Ec3 pulse ...
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bioRxiv - Microbiology 2020Quote: Samples were analyzed directly or mixed 1:1 with one of the following buffers: Quick Extract DNA Extraction Solution (Lucigen), Virotype Tissue Lysis Reagent (INDICAL BIOSCIENCE GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... Comparable results to commercial RNA extraction kits have been obtained using a 5-min direct detection preparation method of nasopharyngeal samples following 1:1 dilution with the Quick Extract DNA extraction Solution (Lucigen) (6) ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of T5-Tn5 transposome and 4 μl of T7-Tn5 transposome were mixed to form 8 μl of assembled Tn5 transposome before it was mixed with 1 μl cDNA (containing ~500 pg cDNA) and 1 μl reaction buffer (TNP92110, Lucigen). The reaction mixture was incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology) ...
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bioRxiv - Genomics 2020Quote: ... 1 ul Hybridase Thermostable RNase H (Lucigen, H39500) and 1 ul 10 X digestion buffer (500 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... using Endura™ ElectroCompetent Cells (Lucigen 60242-1). Resulting pooled DNA libraries were used to produce lentiviral particles and infect primary cortical neurons.
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bioRxiv - Plant Biology 2021Quote: ... coli C41 (DE3) cells (Lucigen, Cat# 60442-1) and purified using Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of 10× stop solution (TNP92110, Lucigen) was added to the reaction mixture to stop tagmentation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μL/mL Ready-Lyse Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Stranded RNA-seq libraries were prepared from 1 μg total RNA using ScriptSeq v2 Complete kit for bacteria (Epicentre) and sequenced on Illumina NovaSeq 6000 platform at GENEWIZ ...
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bioRxiv - Immunology 2022Quote: ... and incubated with a rolling-circle amplification (RCA) reaction mixture (1 unit/μL phi29 polymerase (Lucigen, NxGen kit 30221), 1x phi29 polymerase buffer ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Molecular Biology 2020Quote: ... blunt-end ligation into the pZE21 MCS1 expression vector with constitutive promoter pLteo-1 (Lutz and Bujard, 1997) at the HincII site using the Fast Link ligation kit (Epicentre), and iv ...
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bioRxiv - Genomics 2022Quote: ... Subsequent library preparation was performed with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen®; Cat. no. 14000-1) according to the manufacturer’s instructions with one slight modification ...
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bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2019Quote: ... For RNase III tests 1 unit of enzyme (Epicentre) was added 10 min after addition of RNases I and H ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL/mL Ready-Lyse™ Lysozyme Solution (Lucigen) and 1 μL/mL benzonase nuclease (Sigma) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Electroporations were recovered in 1 mL recovery media (Lucigen) for 1 hour and subsequently grown overnight in LB + selection.
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bioRxiv - Biochemistry 2019Quote: ... and 1 μl of CircLigase I (Epicentre, Madison, WI). Ligation reactions were incubated at 60C for 2 h and the ligase was heat inactivated by incubating at 80C for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μL of Endura electrocompetent bacteria (Lucigen 60242-1) were transformed with 150 ng of library DNA using a 0.1 cm Gene Pulser electroporation cuvette (Bio-Rad 1652083 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from cell pellets as described (Greenstein et al 2018) using the Masterpure yeast RNA extraction kit (Lucigen). cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018 ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was isolated by chloroform extraction and ethanol precipitation and amplified using TargetAmp™ 1-Round aRNA Amplification Kit 103 (Epicentre) according to vendor protocols ...
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bioRxiv - Microbiology 2020Quote: ... in combination with 1 µl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl of Fast-Link DNA ligase (Lucigen, cat#LK0750H), and milliQ water up to 15 μl total volume ...
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bioRxiv - Genetics 2022Quote: ... and 1 uL of CircLigase I (Lucigen CL4111K, Middleton, WI) to the 15 uL of cDNA and incubating at 60 C for 12 hours ...
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bioRxiv - Microbiology 2022Quote: ... in combination with 1 μl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... Immediately after electroporation 1 ml of prewarmed recovery medium (Lucigen, #800261 was added to the bacteria and the cells were incubated for 1 hour at 37 °C shaking at 250 rpm in a bacterial incubator ...
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bioRxiv - Microbiology 2023Quote: ... and Escherichia coli BAC-Optimized Replicator v2.0 (Lucigen; 60210–1), Streptomyces mirabilis P8-A218 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Plant Biology 2020Quote: ... A mixture consisting of 1 μl 10x Circligase reaction buffer (Epicentre), 0.5 μl 1 mM ATP ...
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bioRxiv - Genomics 2021Quote: ... 1.5 μl ATP and 1 μl FastLink DNA ligase (Lucigen Corporation), followed by heat inactivation for 15 min at 70°C ...
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bioRxiv - Genomics 2022Quote: ... including a 1:4 ratio of biotin-16-UTP (BU6105H, Lucigen) to UTP at 5 mM ...
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bioRxiv - Genomics 2022Quote: ... 5 μg RNA were digested with 1 U RNase R (Epicentre) per μg RNA in a total reaction volume of 10 μl for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid library were amplified by electro-transformation (Lucigen, Catalog # 60242-1). Lentivirus library was packaged by co-transfecting HEK293T with psPAX2 and pMD2.G plasmids ...
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bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA was synthesised from 1 µg of total RNA using MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit (Epicentre Biotechnologies, Madison, USA) as per manufacturer’s protocol ...