Labshake search
Citations for Lucigen :
51 - 100 of 651 citations for Bicinchoninic Acid BCA Protein Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2024Quote: The AmpliScribe T7 High Yield Transcription kit (Lucigen) was used for RNA in vitro transcription with a 2-hour incubation at 37°C using gblock sequences available in supplemental ...
-
bioRxiv - Microbiology 2024Quote: ... RNA extracted using MasterPure RNA Purification Kit (Epicentre), and treated with TURBO DNase as described ...
-
bioRxiv - Biochemistry 2024Quote: ... The Circligase II kit (CL9021K) was from Lucigen; the RNA Clean and Concentrator kit (R1019 ...
-
bioRxiv - Genomics 2024Quote: ... using the Shotgun PCR Free Library Kit (Lucigen) and the NovaSeq™ 6000 Sequencing device (Illumina® ...
-
bioRxiv - Microbiology 2024Quote: ... The Ribo-Zero rRNA Removal Kit (Epicentre, USA) was used for DENV2-infected cellular RNA to remove rRNA ...
-
bioRxiv - Biochemistry 2024Quote: ... the EZ-Tn5
transposon kit (Epicentre) and the E54K/M56A/L372P Tn5 hyperactive variant transposase were used for transposon mutagenesis ... -
bioRxiv - Genomics 2022Quote: ... and three libraries for each sex were generated with the NEBNext Ultra RNA library prep kit combined with rRNA depletion via the Ribo-Zero Magnetic Gold kit (Epicentre Biotechnologies). Samples were sequenced on an Illumina HiSeq2500 platform at the Vienna BioCenter Core Facilities (VBCF ...
-
bioRxiv - Microbiology 2023Quote: ... Ribosomal RNA was depleted using the Ribo Zero kit and samples were converted into Illumina sequencing libraries using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre, Illumina). Libraries were sequenced using Illumina HiSeq (2 × 150 bp reads) ...
-
bioRxiv - Genomics 2023Quote: ... Each aliquot of 4 μg was depleted from ribosomal RNA with 10 μl of rRNA removal solution from the Ribo-Zero kit (RiboZero kit, catalog num. MRZH11124, Epicentre-Illlumina), strictly following the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... Purify PCR product using FavorPrep Gel/PCR Purification Kit were used as template for in vitro gRNA production using AmpliScribe T7 Flash Transcription Kit (Epicentre,Lucigen # ASF3257). Then gRNAs were precipitated according to previous detailed protocol and resuspended in nuclease free water28 ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were chilled on ice for 5 minutes and 175 μl of MPC protein precipitation solution (Lucigen, USA) was added to 300 μl of the lysed sample and vortexed vigorously for 10 seconds ...
-
bioRxiv - Developmental Biology 2024Quote: ... Purify PCR product using FavorPrep Gel/PCR Purification Kit were used as template for in vitro gRNA production using AmpliScribe T7 Flash Transcription Kit (Epicentre,Lucigen # ASF3257). Then gRNAs were precipitated according to previous detailed protocol and resuspended in nuclease free water28 ...
-
bioRxiv - Genetics 2020Quote: Total DNA was extracted using a QuickExtract kit (Lucigen). Unless otherwise indicated ...
-
bioRxiv - Genetics 2020Quote: ... and a MasterPure™ Yeast DNA Purification Kit (Lucigen). PE150 libraries of the evolved strains were prepared and sequenced by Novogen.
-
bioRxiv - Molecular Biology 2020Quote: ... we used the MasterPure RNA purification kit from Epicentre instead of phenol/chloroform extraction to purify RNA after the dephosphorylation and ligation steps.
-
bioRxiv - Genetics 2020Quote: ... DNA was isolated using the QuickExtract kit (Epicentre, #QE09050) and the target region in the last exon of ACTN2 (Primers ...
-
bioRxiv - Developmental Biology 2021Quote: Founder mice were genotyped using FailSafe PCR Kit (EpiCentre) and run on a 12% polyacrylamide gel for heteroduplex assay ...
-
bioRxiv - Genetics 2020Quote: ... using Masterpure complete DNA and RNA purification kit (Lucigen), genomic DNA for whole genome sequencing was isolated ...
-
bioRxiv - Plant Biology 2020Quote: ... Ribo-Zero kit (Epicentre, an Illumina company, Madison, WI) was used to remove rRNA from the libraries ...
-
bioRxiv - Microbiology 2021Quote: The ScriptSeq complete kit (Epicentre, Illumina, Madison, WI USA), a combined kit for the ribosomal (rRNA ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... or the Epicentre MasterPure DNA Purification Kit (Epicentre Biotechnologies). All plasmid constructs were built with the Gibson Assembly Master Mix or the NEB Builder HiFi DNA Assembly (both New England Biolabs) ...
-
bioRxiv - Microbiology 2023Quote: ... MasterPureTM Gram positive DNA purification Kit (Epicentre, WI, USA) was used ...
-
bioRxiv - Molecular Biology 2023Quote: Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina) was used to remove ribosomal RNAs and to enrich mRNAs ...
-
bioRxiv - Biochemistry 2023Quote: ... AmpliScribe™ T7-Flash™ Transcription Kit (ASF3507, Lucigen) was used according to the manufacturer protocol with 4 μL PCR products per 20 μL reaction ...
-
bioRxiv - Microbiology 2023Quote: ... A-tailed (Lucigen NxSeq DNA prep kit, Middleton, WI) and ligated to TruSeq adapters (IDT ...
-
bioRxiv - Cell Biology 2021Quote: ... Selected clones lacking the expression of protein-of-interest were sequenced to confirm the knockout: cells were resuspended in QuickExtract (Lucigen), incubated at 65 °C for 15 min ...
-
bioRxiv - Genomics 2022Quote: Shot-gun library was generated at the McGill Genome Center using the NxSeq® AmpFREE Low DNA Library Kit Library Preparation Kit (Lucigen Corp., WI, USA) according to the manufacturer’s recommendations and the library was ran on a HiSeq X for 2×150 cycles.
-
bioRxiv - Microbiology 2023Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI). Remaining steps followed the recommended PureLink Genomic DNA Mini Kit (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... The sample was spun down at 10,000 rcf for 1 min and the supernatant was added to 150 μl of protein precipitation reagent (Epicentre, Lucigen, Middleton, WI) and vortexed for 10s ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s specifications ...
-
bioRxiv - Molecular Biology 2021Quote: ... Libraries were produced using the Art-Seq kit (Epicentre/Illumina) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... with the Ribo-Zero rRNA removal kit for Bacteria (Epicentre) according to standard protocol ...
-
bioRxiv - Systems Biology 2021Quote: RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen), and treated with TURBO DNase (ThermoFischer ...
-
bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA was depleted using Ribo-ZERO magnetic kit (Epicentre). The transcripts were fragmented and used as templates to generate strand-specific cDNA libraries by TruSeq Stranded Total RNA LT Sample Prep kit (Illumina) ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2022Quote: ... The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to make the libraries according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... genomic DNA was extracted with the QuickExtract kit (Epicentre, #QE09050). PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix ...
-
bioRxiv - Microbiology 2021Quote: The ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) was used to prepare directional multiplex libraries ...
-
bioRxiv - Molecular Biology 2020Quote: ... and processing with a RiboZero rRNA removal kit (Epicentre/Illumina) to deplete rRNA ...
-
bioRxiv - Genomics 2021Quote: DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit and sequencing was performed on the Illumina NextSeq500 with 75bp single-end reads (first experiment) ...
-
bioRxiv - Microbiology 2020Quote: ... rRNA was removed using a Ribo-Zero (Bacteria) kit (Epicentre) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... ScriptSeq v2 RNA-Seq library preparation kit (Epicentre Technologies, USA), TruSeq SBS Kit v3-HS (Illumina ...
-
bioRxiv - Genetics 2023Quote: ... DNA was extracted with the MasterPure DNA purification kit (Lucigen). Libraries were prepared using the Illumina TruSeq PCR-free library preparation kit ...
-
bioRxiv - Neuroscience 2022Quote: ... were ligated to the ChIP DNA (Fast-Link kit; Epicentre) and PCR amplified using the Illumina TruSeq ChIP Library Prep Kit ...
-
bioRxiv - Microbiology 2022Quote: ... depleted in ribosomal RNA (Ribo-Zero rRNA Removal Kit, Epicentre) and reverse transcribed into cDNA ...
-
bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA (rRNA) was depleted (Ribo-ZeroTM Gold Kit, Epicentre), cDNA libraries were built with TrueSeq total RNA sample kit (Illumina ...
-
bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was extracted with Master Pure purification kit (Epicentre) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... pombe cells using the MasterPure Yeast RNA Purification Kit (Epicentre). The procedure for removal of DNA contamination was as per the specification of the manufacturer but performed twice to efficiently remove the DNA from the samples ...
-
bioRxiv - Developmental Biology 2024Quote: ... and tailed with a poly(A) polymerase tailing kit (Epicentre) and purified with RNA clean & concentrator-25 kit (Zymo Research).
-
bioRxiv - Genomics 2024Quote: ... using the NxSeq® AmpFREE Low DNA Library Kit (Lucigen) and sequenced to obtain paired-end short reads (150 bp) ...