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Citations for Lucigen :
801 - 850 of 865 citations for Cow Eukaryotic Translation Initiation Factor 1 EIF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Microbiology 2021Quote: ... two ml of a culture grown in R2B were used for extraction of high molecular weight (HMW) DNA using the MasterPureTM DNA Purification Kit (Epicentre, Madison, WI, USA), using the kit’s protocol for cell samples ...
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bioRxiv - Bioengineering 2022Quote: Samples collected at 24 h were processed for DNA isolation using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer’s instructions (MasterPure™ Yeast DNA Purification Kit, Biosearch Technologies - Lucigen, Wisconsin, USA) and purified on Axygen™ AxyPrep Magnetic PCR Clean-up SPRI beads (Axygen Inc ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid extraction from blood samples was performed using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, LGC Ltd, Teddington, GB). For metagenomic analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... eukaryotic mRNA was enriched with Oligo (dT) beads and prokaryotic mRNA was enriched by removing rRNA with Ribo-ZerotM Magnetic Kit (Epicentre, Madison, WI, USA). The enriched mRNA fragments were then split into short fragments using lysates and reverse transcribed into a strand of cDNA using random primers ...
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bioRxiv - Microbiology 2024Quote: ... Digested fragments were cloned into the BamHI/NcoI pre-digested pET15b plasmid using the Fast-Link™ DNA Ligation Kit (Epicentre, Madison, WI), and E ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA (1-3 μg) was then treated with 5 units of RNase R (Lucigen. RNR07250) for one hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 1% Triton X-100) supplemented with 200 U/ml Ready-Lyse lysozyme solution (Lucigen, Middleton, WI) diluted in TE buffer and 1 pill/10 mL Roche cOmplete EDTA-free protease inhibitor cocktail (Millipore sigma ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was carried out with EconoTaq PLUS GREEN 2X Master Mix (cat# 30033-1; Lucigen) and an initial denaturation at 94°C for 2 min ...
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bioRxiv - Genomics 2023Quote: ... Monophosphorylated RNAs were selectively degraded by 1 hour incubation with Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen). Subsequently ...
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bioRxiv - Genomics 2024Quote: ... Monophosphorylated RNAs were selectively degraded by 1 hour incubation with Terminator 5’-Phosphate-Dependent Exonuclease (Lucigen). Subsequently ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... or homogenized tissues were digested with proteinase K prior to DNA isolation using MasterPure™ DNA Purification Kit (Epicentre, by Illumina, Madison, United States) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA from infected barley leaves at 6 days post-inoculation (dpi) was isolated by using the MasterPure™ Complete DNA&RNA purification Kit (Epicentre®, Illumina®) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Isolation of genomic DNA from leaf powder was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre (Epicentre, Chicago, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2024Quote: ... Between 200- 500 ng of enriched mRNA was used for cDNA production using the ScriptSeq®v2RNA-Seq Library Preparation Kit (Epicentre, Madison, WI, USA), FailSafe®PCR Enzyme Mix (Epicentre ...
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bioRxiv - Microbiology 2021Quote: ... 100µl of competent cells was mixed with 1 µl of EZ-Tn5 KAN-2 Tnp Transposome (Epicentre) and electroporated at 1800V ...
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bioRxiv - Developmental Biology 2021Quote: Riboprobes and double-stranded RNA (dsRNA) for src-1 were generated by in vitro transcription (NxGen, Lucigen) as described previously (Petersen and Reddien ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of rRNA-depleted RNA were treated with 1 U Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in the 1x Buffer A in the presence of 40 U RNaseOUT in a 50 μL reaction at 30 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and positive colonies were sequenced ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of purified RNA was used in cDNA synthesis with MMLV HP Reverse Transcriptase (Lucigen RT80125K) and 100 ng random hexamers (IDT) ...
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bioRxiv - Microbiology 2023Quote: ... The pBAC vector that can handle the full-length genome was purchased from Lucigen (cat # 42032-1). This vector was modified to include a CMV promoter ...
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bioRxiv - Microbiology 2021Quote: ... coli chromosome was carried out by electroporating 1 μL of the EZ-Tn5
Tnp Transposome (Epicentre) in 50 μL of EV18-pkD46 electrocompetent cells ... -
bioRxiv - Cell Biology 2021Quote: ... Samples were lysed with 1 ml mammalian lysis buffer containing 200 µl 5x Mammalian Polysome Buffer (Epicentre, RPHMR12126), 100 µl 10% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... we electroporated 1 μL of 25 ng/μL of each library into 50 μL bacteria (Lucigen, 60242-2), with ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg RNA was used for reverse transcription with 0.5 μl MMLV HP reverse transcriptase (Lucigen cat # RT80125K) per 20 μl rxn ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (1 μg) from untreated HPAECs was digested by 2U of RNase R enzyme (Lucigen, Middleton, WI) in 10 μL reactions containing 1x digestion buffer ...
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bioRxiv - Neuroscience 2021Quote: The completed constructs in M-6-attB-UAS-1-3-4 vector were amplified in Epi300 competent cells (EpiCentre) in LB-Chloramphenicol medium ...
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bioRxiv - Genomics 2020Quote: ... then digested with exonuclease I by adding 1 μL of enzyme (20 U/μL) plus 1.5 μL 10X Reaction Buffer (Lucigen) and incubating 1 h at 37 °C ...
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bioRxiv - Systems Biology 2020Quote: ... RNA digestion of lysates was performed for 1 hour with the mixture of 2000 Units of RNase T1 (Epicentre) and 300 Units of RNase S7 (Roche/Sigma) ...
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bioRxiv - Genetics 2023Quote: ... and plasmid replication was induced by diluting 1:10 in LB + 12.5 µl/ml chloramphenicol and 1X CopyControl Induction Solution (Lucigen). The BAC was purified purified using the Monarch Plasmid Miniprep Kit (NEB).
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bioRxiv - Cell Biology 2022Quote: ... Isolated genomic DNA was combined with EconoTaq PLUS GREEN 2X Master Mix (cat# 30033-1; Lucigen, Middleton, WI, USA) and forward and reverse primers (primer information listed in Table S2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The size-selected RNAs or synthetic versions of FLEXIs were subjected to exonuclease treatment (1 U Terminator exonuclease (Lucigen), 20 U RNase R (Abcam) ...
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bioRxiv - Immunology 2019Quote: ... washed once in PBS and genomic DNA was recovered from 1 x 106 cells using 100 μl Quick Extract solution (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... at 37°C (1 – 2 h) and 0.9 μl from the reaction mix were electroporated to E.coli Cloni® 10G cells (Lucigen, USA). Colony PCR and sequencing were used for analysis and verification.
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in Tris-EDTA (TE) buffer (10 mM Tris-Cl, pH 8.0, 1 mM EDTA) containing lysozyme (Epicentre; R1804M) and 1% SDS ...
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bioRxiv - Microbiology 2021Quote: ... flhD or complexed AsflhD with flhD was performed at 37°C in TMN buffer containing 1 µg tRNA for 15 min with RNase III (Epicentre). Samples were loaded on denaturing polyacrylamide gels together with an RNA alkaline ladder as in (75).
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bioRxiv - Genomics 2021Quote: ... 1 μl of the Gibson reaction was delivered to 25 μl of electrocompetent EnduraTM cells (Lucigen, cat. no. 60242-2) using Gene Pulser®/MicroPulser™ Electroporation Cuvettes ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was incubated at 37°C for 30 min and 70°C for 10 min in a 10 μl reaction that contained 1 U RNase R (Lucigen) and 1 μl 10× RNase R buffer ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments in the range of 30-40 kbp were resolved by gel electrophoresis (2 V cm-1 overnight at 4 °C) and recovered from 1% low melting point agarose gel using GELase 50X buffer and GELase enzyme (Epicentre). Nucleic acid fragments were then ligated to the linearized CopyControl pCC2FOS vector following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μg RNA extracted from the specific tethering assays was incubated in a 20 μl reaction volume with 1 unit of Terminator 5’- phosphate-dependent exonuclease (Epicentre) for 60 min at 30°C ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µg of total RNA was mixed with 3 µl of diluted ERCC RNA spike-in mix and then digested for 1h at 30°C with 1 unit of Terminator 5’-Phosphate-Dependent Exonuclease (Epicentre) in 1X Reaction Buffer A containing 10 units of SUPERase-In RNase inhibitor (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was conducted using EconoTaq PLUS 2X Master mix (Cat no. 30035-1, Lucigen, LGC Biosearch technologies, USA) as follow ...
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bioRxiv - Genetics 2021Quote: ... and four CS (line 403 subclones 1, S7, S8, and S9) iPSC lines was extracted using quick extract DNA extraction solution (Epicentre #QE09050), amplified by PCR ...
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bioRxiv - Developmental Biology 2021Quote: ... In brief, RNA was treated with 20 μl RNase R digestion reaction (2 μg RNA, 1 μl RNase R (Lucigen, #RNR07250), 2 μl 10x RNase R reaction buffer ...