Labshake search
Citations for Lucigen :
651 - 700 of 747 citations for Cow Glucosidase 2 Subunit Beta PRKCSH ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the isolates using the MasterPure™ Gram positive DNA extraction kit (Cat. No. MGP04100; Epicentre®). The standard protocol was modified slightly to accommodate for the isolates being Gram negative organisms ...
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bioRxiv - Microbiology 2023Quote: ... A 5 µg aliquot of total RNA was depleted for rRNA with the mRNA then converted into cDNA with the Scriptseq complete bacteria kit (Epicentre). The libraries were sequenced on the Illumina HiSeq platform for 50 bp single end reads.
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using the Promega Maxwell Tissue DNA Kit with the addition of Readylyse Lysozyme Solution (Epicentre) and Lysostaphin (Sigma) ...
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bioRxiv - Genomics 2024Quote: ... Ligated products were pooled and concentrated using the DNA Clean and Concentrator kit before being electroporated into Endura chemically competent cells (Lucigen). The 94-member library was purified using the Midi Plus kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... first-strand cDNAs were synthesized from 1 µg of DNase-treated RNA using the MMLV Reverse Transcriptase Synthesis Kit (Lucigen) protocol with the Oligo(dT)21 primer ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA from isolates exhibiting uracil auxotrophy was purified using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen). The promoter ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was prepared from 3 mL of turbid liquid culture with the MasterPureTM Gram Positive DNA Purification Kit (Lucigen). DNA was quantified by using a NanodropTM device (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from pure yeast colonies with the MasterPure™ Yeast DNA Purification Kit (Epicentre®, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... total DNA and total RNA were extracted from one million cells of the porcine hepatocyte-enriched fraction using MasterPure DNA Purification Kit (Epicentre) and RNAzol RT (Molecular Research Center ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 1 × 107 cells in exponential growth phase using the Masterpure Yeast RNA Purification Kit (Epicentre) according to the manufacturer’s instructions ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Disruption of both alleles of the target locus and integration of the RT were confirmed by PCR after DNA extraction (MasterPure Yeast DNA Purification Kit, Lucigen). The SAT1 gene was removed with the flipper system ...
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bioRxiv - Genetics 2024Quote: ... Fragments containing the biotinylated adaptor were further purified with Dynabeads M-280 streptavidin (Thermo) and DNA ends were repaired using the End-it DNA End-repair kit (Lucigen). P7 adaptors were ligated to DNA fragments ...
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bioRxiv - Cancer Biology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesize RNA complementary DNA ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from two biological replicates from early log phase growth in either YPD or YPD containing 5 mM TUDCA using the MasterPure Yeast RNA purification kit (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from clones following overnight culture using a MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corp., USA). The sequencing library was prepared using the Illumina Nextera kit following published methods (22 ...
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bioRxiv - Genetics 2021Quote: ... the genomic DNA of the pool of cells carrying insertion events was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Cells were lysed using a lysis solution supplemented in zymolyase 20T (1.5 mg/ml) ...
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bioRxiv - Developmental Biology 2020Quote: Library preparation was adapted from previous protocols(Flynn et al., 2015; Ingolia et al., 2012) and the ARTseq Ribosome Profiling Kit manual (Epicentre, Illumina). In summary ...
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
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bioRxiv - Molecular Biology 2020Quote: ... by annealing locus-specific oligonucleotides to a common 5’ universal oligonucleotide and performing in vitro RNA transcription (AmpliScribe T7-Flash Kit, Lucigen, ASF3507)40 ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was performed following the manufacturer’s protocol using the Illumina ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre, USA). Quality of the RNA-Seq transcriptome library was examined using Agilent 2100 High Sensitivity DNA Kit ...
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bioRxiv - Genomics 2020Quote: RNA samples with RNA Integrity Number (RIN) >7.0 were chosen to proceed to rRNA depletion using Ribo-Zero™ rRNA Removal Kits (Bacteria) (Epicentre, USA) prior to cDNA synthesis ...
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bioRxiv - Bioengineering 2020Quote: The 5′-biotinylated-E07 (anti-EGFR aptamer) was generated by performing an in vitro transcription reaction (DuraScribe T7 Transcription Kit, Lucigen, #DS010925), as described previously (Ray et al. ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA collected from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), cleaned with RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 serial dilution samples and a non-evolved yLL132a ancestor with the MasterPure Yeast DNA Purification Kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The gDNA was isolated by using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: DNA was isolated from HTT Q71-GFP-expressing HEK293 cells through MasterPure™ Complete DNA and RNA Purification Kit (Epicentre®) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from mice faecal pellets obtained prior to RVwa inoculation from all experimental groups with the Master Pure™ kit (Epicentre), including a bead beater treatment with 0.5 g of 0.1 mm glass beads in the lysis step (FastPrep 24-5G Homogenizer ...
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bioRxiv - Developmental Biology 2022Quote: ... shRNAs were synthesized by in vitro transcription (IVT) from double stranded DNA templates using the AmpliScribeTM T7-flashTM transcription kit (Lucigen, Inc.) and were purified using Direct-Zol TM RNA Miniprep kits (Zymo Research ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were then used as templates for in vitro transcription per AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies) specifications to obtain dsRNAs.
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bioRxiv - Genomics 2020Quote: ... Dual extraction of nucleic acid (RNA and DNA) from each pupa was carried out using the MasterPure dual extraction kit (Epicentre, MC85200). Briefly ...
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bioRxiv - Microbiology 2023Quote: Chromosomal DNA of the eight suppressor mutants and the original strain (SA5691) was isolated using the MasterPureTM DNA Purification Kit (Epicentre Biotechnologies) according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was repaired with Blunt-end ending 3′ overhang using End-It DNA End-Repair kit in 25 µl volume (Epicentre; ER81050), and 3′-A overhang was added with Klenow fragment (NEB ...
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bioRxiv - Biochemistry 2023Quote: Fosmids from each hit were isolated by BioS&T (Quebec, Canada) or using the FosmidMAX™ DNA Purification Kit (Lucigen Corporation). Isolated fosmids were prepared for Illumina sequencing using the plexWell™ 96 kit (seqWell ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA from overnight saturated cultures of isogenic bacterial clones was prepared using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) according to the manufacturer’s guidelines and sequenced at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Crude genomic DNA was generated by incubating yeast isolates at 95°C in 50 μL of 20 mM NaOH for 10 minutes or the MasterPure™ Yeast DNA Purification Kit (Lucigen). PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega) ...
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bioRxiv - Neuroscience 2024Quote: ... the pGEMHE plasmids carrying different KCR1 variants were linearized by NheI digestion and used for in vitro transcription of complementary RNA (cRNA) using the AmpliCap-Max T7 high-yield message maker kit (Epicentre Biotechnologies). For all the KCR1 expression variants ...
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bioRxiv - Microbiology 2023Quote: ... The gel-recovered fragments were ligated to the linearized PZE12 vector (prime 3,4) by blunt-end ligation enzyme (Epicentre FastLinkTM kit) and ligation products in the range of 4-10 kb were extracted from a 0.7% agarose gel.
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bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... These solutions (5.5 µl each) were then used as template for a 20 µl reaction with the AmpliScribeTM T7 Transcription Kit (Lucigen, AS3107). The reaction product was treated with DNAseI and shRNAs were purified using the RNA Clean and ConcentratorTM Kit (Zymo Reasearch ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was isolated from yeast and contaminating DNA was depleted using the MasterPure Yeast RNA Purification Kit (Epicentre, Madison, WI) protocol with minor changes as previously described (Carrocci et al. ...
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bioRxiv - Genetics 2023Quote: ... at least 30 5-FOA resistant clones were grown in YEPD for DNA extraction by MasterPure™ Yeast DNA Purification Kit (Lucigen). The relative location of each chromosome truncation event was determined using multiplex PCR with primers that anneal centromere or telomere proximal to the SiRTA (File S1) ...
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bioRxiv - Genetics 2023Quote: ... Thirty clones that displayed growth on medium containing 5-FOA were selected and inoculated in liquid YEPD for genomic DNA extraction using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Multiplex PCR was used as described in Ngo et al ...
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bioRxiv - Microbiology 2024Quote: Libraries of class 1 integron gene cassettes were constructed with overnight ligation using the Fast Link ligation kit (Epicentre, Lucigen, USA). Linearized pZE21-Pbla plasmids were ligated with gene cassettes amplified from the metagenomic DNA samples at a 1:5 molar ratio ...
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bioRxiv - Microbiology 2024Quote: Libraries of class 1 integron gene cassettes were constructed with overnight ligation using the Fast Link ligation kit (Epicentre, Lucigen, USA). Linearized pZE21-Pbla plasmids were ligated with gene cassettes amplified from the metagenomic DNA samples at a 1:5 molar ratio ...
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bioRxiv - Immunology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase™ 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesise RNA complementary DNA ...