Labshake search
Citations for Lucigen :
601 - 650 of 706 citations for Rat IL 3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... tissues from secondary GBM (Grade III (n=44), Grade IV (n=23)) and LGG (grade II, n=42) using MasterPure kit (Epicentre). Raw reads from the RNA-seq data were processed using an in-house pipeline that uses STAR for read alignment ...
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bioRxiv - Microbiology 2022Quote: ... the Janus cassette was amplified from genomic DNA isolated from strain AZ59 (13) using the MasterPure DNA purification kit (Lucigen), with flanking regions (∼1kb ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was then stopped with the addition of 190 µl lysis buffer (from MasterPure Yeast DNA Purification Kit (Lucigen)) ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were processed for directional mRNA-seq library construction using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... listed in Table S2 or previously described [89] and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre). All PCR amplified products were confirmed by sequencing (Eurofins MWG Operon ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific libraries were prepared from ribosomal RNA-depleted total RNA using ScriptSeq complete kit for plant leaf (Epicentre; BPL1224) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented gDNA was ended-repaired in 50 μl reaction using the End-It DNA End-Repair Kit (Lucigen, ER81050), incubated at room temperature for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 1 μg purified RNA was applied to RT-qPCR and the remaining was subjected to ribosomal RNA depletion using a Ribo-Zero Gold Kit (Epicentre). Sequencing libraries were constructed from 100 ng total RNA using a Truseq stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: RNA from endodontic samples was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre Technologies, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the isolates using the MasterPure™ Gram positive DNA extraction kit (Cat. No. MGP04100; Epicentre®). The standard protocol was modified slightly to accommodate for the isolates being Gram negative organisms ...
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bioRxiv - Microbiology 2023Quote: ... A 5 µg aliquot of total RNA was depleted for rRNA with the mRNA then converted into cDNA with the Scriptseq complete bacteria kit (Epicentre). The libraries were sequenced on the Illumina HiSeq platform for 50 bp single end reads.
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using the Promega Maxwell Tissue DNA Kit with the addition of Readylyse Lysozyme Solution (Epicentre) and Lysostaphin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... and purified DNA-free RNA samples were subjected to ribosomal depletion with Ribo-Zero™ Magnetic Kits (Epicentre®, Singapore), all according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5
Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ... -
bioRxiv - Microbiology 2023Quote: ... 500uL of the cultured cells were pelleted and the growth media was removed. DNA was extracted using the Master Pure DNA purification kit (Cat No. 85200) from Epicentre. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L ...
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bioRxiv - Biochemistry 2024Quote: The mRNA coding for the catalytic mutant AGOs used in the equilibrium binding assay was transcribed in vitro using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), employing the corresponding pBYL-3×FLAG-SUMO-catalytic mutant AGO plasmids linearized with Not I as templates ...
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bioRxiv - Synthetic Biology 2023Quote: ... was constructed from the linear pSMART-HCKan vector supplied in the Lucigen CloneSmart kit (Lucigen, Middleton WI, Cat#40708-2) with a single ligation step ...
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bioRxiv - Genetics 2023Quote: DNA was isolated from fixed and sorted cells using the MasterPure DNA purification kit (MC85200, Lucigen, LGC Ltd, Teddington, UK) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from clones following overnight culture using a MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corp., USA). The sequencing library was prepared using the Illumina Nextera kit following published methods (22 ...
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bioRxiv - Genetics 2021Quote: ... the genomic DNA of the pool of cells carrying insertion events was extracted using the MasterPure™ Yeast DNA Purification Kit (Lucigen). Cells were lysed using a lysis solution supplemented in zymolyase 20T (1.5 mg/ml) ...
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bioRxiv - Developmental Biology 2020Quote: Library preparation was adapted from previous protocols(Flynn et al., 2015; Ingolia et al., 2012) and the ARTseq Ribosome Profiling Kit manual (Epicentre, Illumina). In summary ...
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bioRxiv - Molecular Biology 2022Quote: ... The linearized plasmids were used as template to generated mRNA by in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106), followed by capping using the ScriptCap m7G Capping System (Cellscript C-SCCE0625) ...
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bioRxiv - Molecular Biology 2020Quote: ... by annealing locus-specific oligonucleotides to a common 5’ universal oligonucleotide and performing in vitro RNA transcription (AmpliScribe T7-Flash Kit, Lucigen, ASF3507)40 ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was performed following the manufacturer’s protocol using the Illumina ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre, USA). Quality of the RNA-Seq transcriptome library was examined using Agilent 2100 High Sensitivity DNA Kit ...
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bioRxiv - Genomics 2020Quote: RNA samples with RNA Integrity Number (RIN) >7.0 were chosen to proceed to rRNA depletion using Ribo-Zero™ rRNA Removal Kits (Bacteria) (Epicentre, USA) prior to cDNA synthesis ...
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bioRxiv - Bioengineering 2020Quote: The 5′-biotinylated-E07 (anti-EGFR aptamer) was generated by performing an in vitro transcription reaction (DuraScribe T7 Transcription Kit, Lucigen, #DS010925), as described previously (Ray et al. ...
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bioRxiv - Neuroscience 2019Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA collected from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), cleaned with RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 serial dilution samples and a non-evolved yLL132a ancestor with the MasterPure Yeast DNA Purification Kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The gDNA was isolated by using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... 50 ml of culture was used for DNA extraction using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, WI, USA).
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bioRxiv - Molecular Biology 2021Quote: DNA was isolated from HTT Q71-GFP-expressing HEK293 cells through MasterPure™ Complete DNA and RNA Purification Kit (Epicentre®) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from mice faecal pellets obtained prior to RVwa inoculation from all experimental groups with the Master Pure™ kit (Epicentre), including a bead beater treatment with 0.5 g of 0.1 mm glass beads in the lysis step (FastPrep 24-5G Homogenizer ...
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bioRxiv - Developmental Biology 2022Quote: ... shRNAs were synthesized by in vitro transcription (IVT) from double stranded DNA templates using the AmpliScribeTM T7-flashTM transcription kit (Lucigen, Inc.) and were purified using Direct-Zol TM RNA Miniprep kits (Zymo Research ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg of RNA was used as template for reverse transcription following the manufacturer’s instructions (EpiScript™ Reverse Transcriptase System kit, Epicentre, USA), using random hexamer primers (Fermentas ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were then used as templates for in vitro transcription per AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies) specifications to obtain dsRNAs.
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was isolated by chloroform extraction and ethanol precipitation and amplified using TargetAmp™ 1-Round aRNA Amplification Kit 103 (Epicentre) according to vendor protocols ...
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bioRxiv - Genomics 2020Quote: ... Dual extraction of nucleic acid (RNA and DNA) from each pupa was carried out using the MasterPure dual extraction kit (Epicentre, MC85200). Briefly ...
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bioRxiv - Genomics 2019Quote: DNA for ChIP and other high throughput sequencing approaches was processed as follows: End repair was performed using an Epicenter End-It kit (Lucigen ER0720), according to manufacturer’s instructions and column purified in a Zymo Minelute column (Zymo D4013) ...
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bioRxiv - Developmental Biology 2022Quote: ... These solutions (5.5 µl each) were then used as template for a 20 µl reaction with the AmpliScribeTM T7 Transcription Kit (Lucigen, AS3107). The reaction product was treated with DNAseI and shRNAs were purified using the RNA Clean and ConcentratorTM Kit (Zymo Reasearch ...
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bioRxiv - Plant Biology 2022Quote: ... The first strand of cDNA was synthesized from total RNA (2 μg) using the NxGen M-MuLV Reverse Transcriptase cDNA synthesis kit (Lucigen, UK). The qPCR consisted of a final volume of 15 μl containing 10 ng of cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: Yeast genomic DNA was prepared from 5 mL stationary phase culture either with the MasterPure™ Yeast DNA Purification Kit (Lucigen) according to the manufacturer guidelines or using the Cetyl Trimethyl Ammonium Bromide (CTAB ...
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bioRxiv - Biochemistry 2022Quote: ... the CleanNGS elute was adjusted to 25ul with 10mM Tris pH 7.5 and the ends of the digested DNA were repaired and phosphorylated at their 5’ end using the End-It DNA End-repair kit (Lucigen #ER0720). DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 ng DNase I treated total RNA was poly-A tailed using the poly-A tailing kit from Epicentre (Madison, WI). Since miR-26a-3p or miR-27a-3p had similar abundance in acini and islets ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA from overnight saturated cultures of isogenic bacterial clones was prepared using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) according to the manufacturer’s guidelines and sequenced at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Crude genomic DNA was generated by incubating yeast isolates at 95°C in 50 μL of 20 mM NaOH for 10 minutes or the MasterPure™ Yeast DNA Purification Kit (Lucigen). PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega) ...