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Citations for Lucigen :
601 - 631 of 631 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Evolutionary Biology 2023Quote: The shRNA and mimiRs were transcribed according to the manufacturer’s instructions using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen, USA) with a few changes ...
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bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Systems Biology 2024Quote: Plasmid DNA was extracted both for MNase-activated cells and non-activated cells using the MasterPure Yeast DNA Purification Kit (Lucigen Corporation, MPY80200), with some modifications ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stored either in 30 μL 2xT&C lysis buffer of the MasterPure Complete DNA & RNA Purification Kit (Lucigen, Wisconsin, USA) or in 10 μL PCR-grade water ...
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bioRxiv - Developmental Biology 2020Quote: We used 200-250 primordial follicles and 100-150 primary follicles for cDNA synthesis using the Message Booster cDNA synthesis kit (Lucigen, Palo Alto, CA). Direct cDNA and subsequent cRNA syntheses were performed by following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... subtilis lysine riboswitch and Tetrahymena ribozyme were also produced by in vitro transcription using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). All constructs were 5’ capped using vaccinia capping enzyme (NEB-M2080S ...
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bioRxiv - Immunology 2021Quote: 200ul aliquots of blood samples collected on GD15 were used for genomic DNA (gDNA) extraction for bisulfite pyrosequencing using MasterPure DNA Purification kits (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Microbiology 2021Quote: ... Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc., Madison, WI, USA) in accordance with the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: 20 µg RNA from each sample was depleted of rRNA using a Ribo-Zero rRNA Magnetic Kit (Epicentre, St Louis, Missouri, United States) including the optional RiboGuard RNase inhibitor according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... for the top four guides for construction of the gRNA and were used for in vitro transcription using the AmpliScribe T7-Flash Transcription kit (Lucigen, Palo Alto, CA) and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... eDNA was purified from the samples by removing the proteins and RNA using MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), and the DNA concentration was measured using NanoVue Plus (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Fosmid libraries of peat lake and freshwater samples were constructed with a pCC2Fos copy control library kit according to manufacturer’s protocol (Epicentre Biotechnologies, Cat. No. CCFOS059), and 198 96-well plates (66 from each sampling location ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA from the whole mosquito was isolated and purified using the MasterPure™ Gram Positive DNA Purification Kit following the manufacturer’s instructions (Epicentre Biotechnologies, Madison, USA). During DNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Microbiology 2021Quote: ... two ml of a culture grown in R2B were used for extraction of high molecular weight (HMW) DNA using the MasterPureTM DNA Purification Kit (Epicentre, Madison, WI, USA), using the kit’s protocol for cell samples ...
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bioRxiv - Bioengineering 2022Quote: Samples collected at 24 h were processed for DNA isolation using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA). Briefly ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid extraction from blood samples was performed using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, LGC Ltd, Teddington, GB). For metagenomic analysis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer’s instructions (MasterPure™ Yeast DNA Purification Kit, Biosearch Technologies - Lucigen, Wisconsin, USA) and purified on Axygen™ AxyPrep Magnetic PCR Clean-up SPRI beads (Axygen Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... eukaryotic mRNA was enriched with Oligo (dT) beads and prokaryotic mRNA was enriched by removing rRNA with Ribo-ZerotM Magnetic Kit (Epicentre, Madison, WI, USA). The enriched mRNA fragments were then split into short fragments using lysates and reverse transcribed into a strand of cDNA using random primers ...
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bioRxiv - Microbiology 2024Quote: ... Digested fragments were cloned into the BamHI/NcoI pre-digested pET15b plasmid using the Fast-Link™ DNA Ligation Kit (Epicentre, Madison, WI), and E ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... or homogenized tissues were digested with proteinase K prior to DNA isolation using MasterPure™ DNA Purification Kit (Epicentre, by Illumina, Madison, United States) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA from infected barley leaves at 6 days post-inoculation (dpi) was isolated by using the MasterPure™ Complete DNA&RNA purification Kit (Epicentre®, Illumina®) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Isolation of genomic DNA from leaf powder was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre (Epicentre, Chicago, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Swabs were incubated for one hour at 37°C with shaking in 300μL yeast cell lysis solution (from Lucigen MasterPure Yeast DNA Purification kit) and 10,000 units of ReadyLyse Lysozyme solution (Lucigen) ...
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bioRxiv - Microbiology 2024Quote: ... Between 200- 500 ng of enriched mRNA was used for cDNA production using the ScriptSeq®v2RNA-Seq Library Preparation Kit (Epicentre, Madison, WI, USA), FailSafe®PCR Enzyme Mix (Epicentre ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by using the instructions for cell samples of the MasterPure™ Complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).