Labshake search
Citations for Lucigen :
601 - 650 of 655 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... DNA was repaired with Blunt-end ending 3′ overhang using End-It DNA End-Repair kit in 25 µl volume (Epicentre; ER81050), and 3′-A overhang was added with Klenow fragment (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and rRNA was removed from 1 mg of total RNA with Ribo-Zero Magnetic Gold Kit (Epicentre Biotechnologies, Madison, WI, USA). To construct the RNA-Seq library ...
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bioRxiv - Molecular Biology 2024Quote: ... by annealing locus-specific oligonucleotides to a common 5’ universal oligonucleotide and performing in vitro RNA transcription (AmpliScribe T7-Flash Kit, Lucigen, ASF3507)(Gagnon et al ...
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bioRxiv - Synthetic Biology 2024Quote: Genomic DNA from overnight saturated cultures of isogenic bacterial clones was prepared using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) according to the manufacturer’s guidelines and sequenced at SeqCenter (Pittsburgh ...
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bioRxiv - Microbiology 2024Quote: Libraries of class 1 integron gene cassettes were constructed with overnight ligation using the Fast Link ligation kit (Epicentre, Lucigen, USA). Linearized pZE21-Pbla plasmids were ligated with gene cassettes amplified from the metagenomic DNA samples at a 1:5 molar ratio ...
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bioRxiv - Microbiology 2024Quote: Libraries of class 1 integron gene cassettes were constructed with overnight ligation using the Fast Link ligation kit (Epicentre, Lucigen, USA). Linearized pZE21-Pbla plasmids were ligated with gene cassettes amplified from the metagenomic DNA samples at a 1:5 molar ratio ...
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bioRxiv - Microbiology 2024Quote: ... 3 mL of saturated culture was pelleted and used according to manufacturer instructions following the MasterPure Complete DNA and RNA Purification Kit (Lucigen MC85200). Isolated genomic DNA was quantified using a NanoDrop and used as input for the Illumina DNA Prep Tagmentation Kit (formerly Nextera DNA Flex ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribo-Zero treated RNA was used to create multiplexed RNA-seq libraries using the ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase™ 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesise RNA complementary DNA ...
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bioRxiv - Immunology 2024Quote: ... complementary DNA (cDNA) synthesis was conducted by using MMLV Reverse Transcriptase™ 1st-Strand cDNA Synthesis Kit (Lucigen, Middleton, Wisconsin, USA) to synthesise RNA complementary DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... whole genome sequencing was performed on DNA isolated from approximately 30 OD600 yeast cells using Lucigen MasterPure Yeast DNA Purification kit (Lucigen catalog: MMPY80200) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2020Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, Message BOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Genetics 2020Quote: The DNA isolation was performed on the swim up purified sperm using the MasterPure DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA) as previously described [13] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The reverse complement DNA templates were ordered from Integrated DNA Technologies (USA) and transcribed using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen). The DNase treated products were cleaned with Quick-RNA MiniPrep Kit (Zymo Research) ...
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bioRxiv - Microbiology 2021Quote: ... and the phage pellet used for phage DNA preparation with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
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bioRxiv - Genomics 2022Quote: ... from at least two freshly starved 9 cm plates of the appropriate worm strain using the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), following manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: High molecular weight DNA extracted from all enrichment cultures was combined in equimolar amounts and used to prepare two metagenomic fosmid libraries ‘IS_Lib1’ (Cavascura enrichments) and ‘IS_Lib2’ (Maronti enrichments) using the CopyControl™ Fosmid Library pCC2FOS Production Kit (Epicentre Technologies, Madison, USA). DNA was end-repaired to generate blunt-ended 5’-phosphorylated fragments according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... ribosomal RNA was removed from 3 μg of total RNA per sample using the Epicentre Ribo-zero™ rRNA Removal Kit (pig; Epicentre, USA). Strand-specific libraries were generated from the rRNA-depleted RNA using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Individual colonies were selected and subcultured in THM to stationary phase and genomic DNA prepared using the Masterpure Gram-positive DNA purification kit (Lucigen, Middleton, WI). The samples then were purified further using ZymoDNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... primer sets were screened against a synthetic TSV RNA target in vitro transcribed from gBlock™ DNA (IDT, Table S1) using the Ampliscribe T7-Flash Transcription kit (Lucigen Corporation) and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and the pellet resuspended in 300 μl of lysis solution and 1 μl of RNase A from the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, US). The kit protocol was followed ...
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bioRxiv - Evolutionary Biology 2023Quote: The shRNA and mimiRs were transcribed according to the manufacturer’s instructions using the AmpliScribe™ T7-Flash™ Transcription kit protocol (Lucigen, USA) with a few changes ...
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bioRxiv - Microbiology 2023Quote: ... and the kanamycin resistance gene (KanR) of the transposon of the EZ-Tn5™
Insertion Kit (Lucigen, Wisconsin, USA) via overlap extension PCR by using primers list in Supplementary Table3 ... -
bioRxiv - Systems Biology 2024Quote: Plasmid DNA was extracted both for MNase-activated cells and non-activated cells using the MasterPure Yeast DNA Purification Kit (Lucigen Corporation, MPY80200), with some modifications ...
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bioRxiv - Microbiology 2024Quote: ... Cells were stored either in 30 μL 2xT&C lysis buffer of the MasterPure Complete DNA & RNA Purification Kit (Lucigen, Wisconsin, USA) or in 10 μL PCR-grade water ...
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bioRxiv - Developmental Biology 2020Quote: We used 200-250 primordial follicles and 100-150 primary follicles for cDNA synthesis using the Message Booster cDNA synthesis kit (Lucigen, Palo Alto, CA). Direct cDNA and subsequent cRNA syntheses were performed by following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... subtilis lysine riboswitch and Tetrahymena ribozyme were also produced by in vitro transcription using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). All constructs were 5’ capped using vaccinia capping enzyme (NEB-M2080S ...
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bioRxiv - Immunology 2021Quote: 200ul aliquots of blood samples collected on GD15 were used for genomic DNA (gDNA) extraction for bisulfite pyrosequencing using MasterPure DNA Purification kits (Lucigen, Middleton, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Microbiology 2021Quote: ... Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc., Madison, WI, USA) in accordance with the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: 20 µg RNA from each sample was depleted of rRNA using a Ribo-Zero rRNA Magnetic Kit (Epicentre, St Louis, Missouri, United States) including the optional RiboGuard RNase inhibitor according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... for the top four guides for construction of the gRNA and were used for in vitro transcription using the AmpliScribe T7-Flash Transcription kit (Lucigen, Palo Alto, CA) and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... eDNA was purified from the samples by removing the proteins and RNA using MasterPure Gram Positive DNA Purification Kit (Epicentre, Madison, WI, USA), and the DNA concentration was measured using NanoVue Plus (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Fosmid libraries of peat lake and freshwater samples were constructed with a pCC2Fos copy control library kit according to manufacturer’s protocol (Epicentre Biotechnologies, Cat. No. CCFOS059), and 198 96-well plates (66 from each sampling location ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA from the whole mosquito was isolated and purified using the MasterPure™ Gram Positive DNA Purification Kit following the manufacturer’s instructions (Epicentre Biotechnologies, Madison, USA). During DNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... cells were pelleted via centrifugation (> 10,000g for 4 min.) and their genomic DNA was extracted using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corporation, Middleton, WI), according to manual’s instruction.
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bioRxiv - Microbiology 2021Quote: ... two ml of a culture grown in R2B were used for extraction of high molecular weight (HMW) DNA using the MasterPureTM DNA Purification Kit (Epicentre, Madison, WI, USA), using the kit’s protocol for cell samples ...
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bioRxiv - Bioengineering 2022Quote: Samples collected at 24 h were processed for DNA isolation using the Epicentre MasterPure Gram Positive DNA Purification Kit (Lucigen, Middleton, WI, USA). Briefly ...
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bioRxiv - Synthetic Biology 2022Quote: ... The purified template was transcribed in vitro at 37 °C for 1 h using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: The purified template was transcribed in vitro at 37 °C for 1 h and 30 min using Ampliscribe™ T7-Flash™ Transcription kit (Lucigen, Epicentre). After DNase treatment at 37 °C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid extraction from blood samples was performed using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, LGC Ltd, Teddington, GB). For metagenomic analysis ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer’s instructions (MasterPure™ Yeast DNA Purification Kit, Biosearch Technologies - Lucigen, Wisconsin, USA) and purified on Axygen™ AxyPrep Magnetic PCR Clean-up SPRI beads (Axygen Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... eukaryotic mRNA was enriched with Oligo (dT) beads and prokaryotic mRNA was enriched by removing rRNA with Ribo-ZerotM Magnetic Kit (Epicentre, Madison, WI, USA). The enriched mRNA fragments were then split into short fragments using lysates and reverse transcribed into a strand of cDNA using random primers ...
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bioRxiv - Microbiology 2024Quote: ... Digested fragments were cloned into the BamHI/NcoI pre-digested pET15b plasmid using the Fast-Link™ DNA Ligation Kit (Epicentre, Madison, WI), and E ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... or homogenized tissues were digested with proteinase K prior to DNA isolation using MasterPure™ DNA Purification Kit (Epicentre, by Illumina, Madison, United States) according to manufacturer’s instructions.