Labshake search
Citations for Lucigen :
551 - 600 of 701 citations for Mouse Leptin ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Insert-DNA (50 ng) was ligated with vector-DNA (150 ng) using the Fast-Link DNA Ligation Kit (LK0750H, Lucigen). XL10-Gold ultracompetent cells were transformed with the ligation product and streaked out on agar plates containing Kanamycin ...
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bioRxiv - Plant Biology 2021Quote: ... 50 ng of rRNA-depleted RNA was used to construct sequencing libraries using a ScriptSeq v2 RNA-seq Library Preparation Kit (SSV21124, Epicentre). Twelve PCR cycles were used for amplification of the libraries ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA molecules were depleted from the total RNA samples using the Ribo-Zero rRNA Removal Kit for bacteria (Epicentre), and small RNAs (< 200 nt ...
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bioRxiv - Neuroscience 2020Quote: DNA from six PVN from each supplemental tactile stimulation group in the repeated room temperature condition and nest temperature condition (total n = 24) was extracted using the Masterpure Complete DNA/RNA Extraction kit (Epicentre) and 300 ng of DNA was used for bisulfite conversion using the Epitect Fast Bisulfite Conversion kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... GU332722.1) or Asia II 1 (GenBank accession no. GU332721.1) was synthesized using the AmpliScribe T7- Flash Transcription Kit (Epicentre, ASF3507), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from cell pellets as described (Greenstein et al 2018) using the Masterpure yeast RNA extraction kit (Lucigen). cDNA was produced from 2-3 g total RNA as described (Greenstein et al 2018 ...
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bioRxiv - Microbiology 2021Quote: ... and further used for the cDNA synthesis by using ScriptSeq™ v2 RNA-Seq kit (Epicentre, Illumina, Madison, WI USA). The cDNA was purified using AMPure XP (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted from the frozen tissues (10 mg) with a MasterPure Complete DNA and RNA purification Kit (Lucigen). We used Q5®High-Fidelity 2X Master Mix (NEB ...
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bioRxiv - Microbiology 2022Quote: ... The resulting air-dried pellets were resuspended in RNase-free water and 3 µg of DNA from each sample was depleted of ribosomal RNA using the Bacteria Ribo-Zero rRNA Removal Kit (Epicentre) and purified using the RNeasy miniprep kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: ... Subsequent library preparation was performed with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen®; Cat. no. 14000-1) according to the manufacturer’s instructions with one slight modification ...
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bioRxiv - Plant Biology 2021Quote: ... 4 μg of purified RNA was depleted of ribosomal RNA using a Ribo-Zero rRNA Removal Kit - Plant Leaf (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The library prep was performed according to the manufacturer’s instructions for the NxSeq® AmpFREE Low DNA Library Kit from Lucigen® (Cat No ...
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bioRxiv - Molecular Biology 2020Quote: ... Metatranscriptomic libraries were prepared for sequencing with the addition of 5–50 ng of RNA to the ScriptSeq cDNA V2 library preparation kit (Epicentre). Metatranscriptomic samples were sequenced with an Illumina NextSeq 500 system using V2 high output 300 cycle reagent kit with PHIX control added ...
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bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... A library of ∼500,000 independent mini-Tn5 transposon mutants was created by transforming BW25113 ΔascA with an EZ-Tn5
Tnp transposome kit (Epicentre) by electroporation ... -
bioRxiv - Molecular Biology 2020Quote: ... fragmentation of extracted DNA into pieces of an average size of 2 kb by sonication using a Covaris E210 (Massachusetts, USA) followed by end-repair using the End-It end repair kit (Epicentre), iii ...
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bioRxiv - Molecular Biology 2022Quote: ... Fig 5B-D and Fig EV5 were obtained as follows: Total RNA was extracted from cells using the MasterPure Yeast RNA Purification Kit including a DNase treatment step (Epicentre), and converted to cDNA using random primers and the RevertAid Reverse Transcriptase kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Half of the cells in each well were used to purify genomic DNA via the Epicentre MasterPure DNA Purification Kit (Epicentre). PCR was used to amplify the CRISPR reaction locus with designed primer pairs ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1.5 μg of RNA per sample was used as input material for rRNA removal using the Ribo-Zero rRNA Removal Kit (Epicentre). cDNA libraries were generated using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted with Total RNA Zol-Out Kit (A&A Biotechnology, Poland) and converted to cDNA using NxGen M-MulV reverse transcriptase (Lucigen) with random primers (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Microbiology 2020Quote: The DNA isolation procedure and WGS procedure was performed as follows: DNA was extracted using the MasterPure DNA isolation kit (Lucigen) or MasterPure Gram Positive DNA purification kit (Lucigen) ...
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bioRxiv - Microbiology 2021Quote: ... Phage and bacterial genomic DNA were prepared with the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA).
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bioRxiv - Microbiology 2019Quote: A genomic library of Pseudoalteromonas issachenkonii (isolate no. 91) was constructed using the Copy Control Fosmid Library Production Kit and vector pCC1FOS (Epicentre) with the modifications described by Weiland et al ...
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bioRxiv - Genetics 2020Quote: ... and used as a template for in-vitro transcription of gRNA by AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies).
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bioRxiv - Neuroscience 2020Quote: ... RNA samples with RNA integrity numbers (RIN) greater than seven (mean RIN 8.5) were processed with Ribo-Zero Gold kit (Epicentre, WI) to remove ribosomal RNA ...
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bioRxiv - Cell Biology 2022Quote: ... are listed in Table S3 and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre) from budding cells and cells incubated with serum ...
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bioRxiv - Biochemistry 2021Quote: ... pure DNA was isolated by using the MasterPure™ Gram Positive DNA Purification Kit (Epicentre, Illumina, San Diego, CA, USA) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from harvested cultures using the Masterpure™ Complete DNA and RNA Kit (Lucigen Corporation, Middleton, Wisconsin). RNA was subsequently purified using the Zymo RNA Clean and Concentrator™ Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: ... or XhoI- (for plasmids with the prefix “pUC57-”) digested plasmids or PCR products using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), followed by capping with ScriptCap m7G Capping System (Cell Script) ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted directly from the sorted nuclei without centrifugation using the MasterPure DNA Extraction kit (Epicentre, Madison, Wisconsin, USA) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... tissues from secondary GBM (Grade III (n=44), Grade IV (n=23)) and LGG (grade II, n=42) using MasterPure kit (Epicentre). Raw reads from the RNA-seq data were processed using an in-house pipeline that uses STAR for read alignment ...
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bioRxiv - Microbiology 2022Quote: ... the Janus cassette was amplified from genomic DNA isolated from strain AZ59 (13) using the MasterPure DNA purification kit (Lucigen), with flanking regions (∼1kb ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was then stopped with the addition of 190 µl lysis buffer (from MasterPure Yeast DNA Purification Kit (Lucigen)) ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were processed for directional mRNA-seq library construction using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... listed in Table S2 or previously described [89] and RNA extraction was carried out using Master Pure yeast RNA extraction purification kit (Epicentre). All PCR amplified products were confirmed by sequencing (Eurofins MWG Operon ...
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bioRxiv - Synthetic Biology 2022Quote: For isolation of genomic DNA (gDNA) of phages Master-Pure™ Complete DNA and RNA Purification Kit (Lucigen, Wisconsin, USA) was used according to the supplier’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Strand-specific libraries were prepared from ribosomal RNA-depleted total RNA using ScriptSeq complete kit for plant leaf (Epicentre; BPL1224) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragmented gDNA was ended-repaired in 50 μl reaction using the End-It DNA End-Repair Kit (Lucigen, ER81050), incubated at room temperature for 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 1 μg purified RNA was applied to RT-qPCR and the remaining was subjected to ribosomal RNA depletion using a Ribo-Zero Gold Kit (Epicentre). Sequencing libraries were constructed from 100 ng total RNA using a Truseq stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Biochemistry 2024Quote: The mRNA coding for the catalytic mutant AGOs used in the equilibrium binding assay was transcribed in vitro using the AmpliScribe T7 High Yield Transcription Kit (Lucigen), employing the corresponding pBYL-3×FLAG-SUMO-catalytic mutant AGO plasmids linearized with Not I as templates ...
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bioRxiv - Microbiology 2023Quote: ... 500uL of the cultured cells were pelleted and the growth media was removed. DNA was extracted using the Master Pure DNA purification kit (Cat No. 85200) from Epicentre. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L ...
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bioRxiv - Microbiology 2023Quote: ... These electrocompetent cells were transformed with the transposon Tn5::kan (EZ-Tn5™
Tnp Transposome™ Kit, Lucigen) following the indications of the manufacture ... -
bioRxiv - Microbiology 2022Quote: RNA from endodontic samples was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre Technologies, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2) The product from assembly reaction was purified and electroporated into Endura Electrocompetent cells (Lucigen). Transformed bacteria were diluted 1:100 in 2xYT medium containing 100 μg/ml carbenicillin (Sigma ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the isolates using the MasterPure™ Gram positive DNA extraction kit (Cat. No. MGP04100; Epicentre®). The standard protocol was modified slightly to accommodate for the isolates being Gram negative organisms ...
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bioRxiv - Microbiology 2023Quote: ... A 5 µg aliquot of total RNA was depleted for rRNA with the mRNA then converted into cDNA with the Scriptseq complete bacteria kit (Epicentre). The libraries were sequenced on the Illumina HiSeq platform for 50 bp single end reads.
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was extracted using the Promega Maxwell Tissue DNA Kit with the addition of Readylyse Lysozyme Solution (Epicentre) and Lysostaphin (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... was constructed from the linear pSMART-HCKan vector supplied in the Lucigen CloneSmart kit (Lucigen, Middleton WI, Cat#40708-2) with a single ligation step ...
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bioRxiv - Genetics 2023Quote: DNA was isolated from fixed and sorted cells using the MasterPure DNA purification kit (MC85200, Lucigen, LGC Ltd, Teddington, UK) following the manufacturer’s protocol ...