Labshake search
Citations for Lucigen :
451 - 500 of 744 citations for rno mir 134 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA (rRNA) was depleted from each RNA sample using the Ribo-ZerorRNA Removal Kit (Epicentre), designed for human ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from the homogenized sample using the MasterPure Complete DNA & RNA Purification Kit (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: A transposon mutant library in pJBG007 was made using EZ-Tn5
Insertion Kit (Lucigen). The following components were used for a 10µl reaction (EZ-Tn5 10X Reaction Buffer (1µl ... -
bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Microbiology 2019Quote: ... Total genomic DNA was extracted from 200 μl of blood using a MasterPure DNA purification kit (Epicentre® Biotechnologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries of ribosome protected fragments were generated using the ARTseq™ Ribosome Profiling Kit (Epicentre, RPHMR12126) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... We first repaired the purified DNA again using the End-it DNA End-Repair Kit (Lucigen #ER0720) following the manufacturer’s suggested conditions ...
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bioRxiv - Genomics 2020Quote: Genomic DNA from 300 million cells was purified using the Master Pure Yeast DNA Purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: The transposon used for genome wide insertion is derived from Ez-Tn5™ custom transposon kits (Epicentre). The insert is a cassette composed of the resistance gene to kanamycin and the gfpmut2 gene under control of the well characterized ribosomal promoter rrnBP1 ...
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bioRxiv - Molecular Biology 2019Quote: 5 µg of extracted RNA was depleted from ribosomal RNA using Ribo-Zero Gold Kit (Epicentre Madison). After fragmentation of the rRNA-depleted RNA ...
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bioRxiv - Genomics 2019Quote: ... coli RNA that underwent reverse transcription and amplification using the ScriptSeq Complete Gold Kit for Epidemiology (Epicentre).
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bioRxiv - Molecular Biology 2020Quote: ... The ‘curlcake’ constructs were in vitro transcribed using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507) with either unmodified rNTPs (UNM) ...
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bioRxiv - Biochemistry 2021Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre, Madison, WI). These pools of barcoded RNAs were converted to Illumina cDNA libraries in three main steps ...
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bioRxiv - Neuroscience 2020Quote: ... mRNAs were purified after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre Biotech., WI). Fluorescently labeled cRNAs derived from these transcripts were used to probe Agilent Mouse V4.0 LncRNA Array containing probes for 22,692 mRNAs (ArrayStar ...
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bioRxiv - Genomics 2020Quote: ... and were used as templates for in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106). The mRNAs were then capped in a subsequent reaction using the ScriptCap m7G Capping System (Cellscript C-SCCE0625).
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bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Microbiology 2020Quote: The genomic DNA from the mycelia was extracted using the MasterPure™ Yeast DNA Purification kit (Lucigen) and used subsequently for PCR amplification ...
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bioRxiv - Evolutionary Biology 2022Quote: ... this mixture was mixed with the components of AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen, USA) and incubated for eight hours at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from 1.5 mL of logarithmically growing cells (OD600 ~0.6-0.8) using the Masterpure Yeast RNA Purification kit (Epicentre). Residual genomic DNA was removed using the Turbo DNA-free kit (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... DNA was isolated from overnight cultures using a MasterPure™ Gram Positive DNA Purification Kit (Epicentre, USA). Nanopore libraries were prepared using Ligation Sequencing Kit (SQK-RBK109 ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from 154 clinical and environmental samples using the MasterPure™ DNA Purification Kit (Epicentre), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... 50ng ribo-depleted RNA was used for library preparation with the Scriptseq kit (Epicentre, cat. no. SSV21124) following the manufacturer’s protocol but with the following modifications ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of shRNAs was performed using the AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen). The transcription reaction was allowed to continue at 37°C for 5-7h ...
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bioRxiv - Molecular Biology 2020Quote: Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA ...
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bioRxiv - Microbiology 2019Quote: ... Barcoded RNAs were pooled and depleted of rRNA using the RiboZero rRNA depletion kit (Epicentre, Madision, W.I.). Pools of barcoded RNAs were converted to Illumina cDNA libraries in 2 main steps ...
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bioRxiv - Evolutionary Biology 2020Quote: ... jirovecii-infected patients was extracted using the MasterPure Yeast DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA). Total RNAs for P ...
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bioRxiv - Cancer Biology 2021Quote: ... while prokaryotic mRNA was enriched by removing rRNA by Ribo-Zero™ Magnetic Kit (Epicentre, Madison, USA). Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers ...
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bioRxiv - Microbiology 2022Quote: The RNAs from Mucor strains were extracted using the MasterPure™ yeast RNA purification kit (Lucigen MPY03100). Extracted RNA was then converted to cDNA by utilizing a high-capacity cDNA reverse transcription kit (Applied Biosystems™ 4374966) ...
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bioRxiv - Bioengineering 2020Quote: ... and had genetic material isolated via the MasterPure™ genomic DNA purification kit (Lucigen, Cat. No. MC85200). Genomic DNA was fragmented to 300 bp via Covaris sonication ...
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bioRxiv - Pathology 2021Quote: ... then gDNA was extracted using the MasterPure Gram Positive DNA Purification kit according to manufacturer’s protocol (Lucigen). Library preparation and sequencing was performed by the Microbial Genome Sequencing Center (MiGS) ...
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bioRxiv - Genomics 2020Quote: The gDNA used in this study was extracted with the MasterPure™ DNA Purification Kit (Epicentre, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was extracted independently for each specimen using the MasterPure Complete DNA and RNA purification Kit (Epicentre) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... total RNA was subjected to rRNA-depletion using RiboZeroTM rRNA Removal Kit (EpiCentre Inc., Madison, WI, USA). Double stranded cDNA synthesis was performed with rRNA-depleted mRNA using ScriptSeqTM v2 RNA-Seq Library Preparation kit (EpiCentre Inc. ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from pure colonies using the MasterPure™Yeast DNA Purification Kit (Epicentre, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... gDNA was directly isolated from conidia stocks using the MasterPure(tm) Yeast DNA Purification Kit (Lucigen/Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... gDNA was directly isolated from conidia stocks using the MasterPure(tm) Yeast DNA Purification Kit (Lucigen/Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and genomic DNA was extracted from them using the MasterPure DNA Purification kit (Epicentre, Madison, WI, USA). Genomic DNA from one additional human iPSC (AG25370 ...
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bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
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bioRxiv - Genetics 2022Quote: ... MTR4 or mtr4-1 cells was isolated using the MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen). Cells were incubated in 2 mL of Leu-minimal medium at 30°C and grown to saturation overnight ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the samples was isolated using MasterPure Complete DNA & RNA Purification Kit (Lucigen, Middleton, WI) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from flies using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen) and converted to cDNA with the PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: Ribosomal RNAs (rRNA) were removed using the Epicentre Ribo-zero® rRNA Removal Kit (Epicentre, Madison, WI). For the whole biopsies ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted with the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, Cat. No.: MC85200). The Qubit 3.0 fluorometer and Nano-300 (Allsheng™ ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA of 2 mL overnight culture was extracted with the MasterPure™ Yeast DNA Purification Kit (Lucigen) and whole PCR Tag analysis was performed on population level to test the general presence of all tRNAs within the population (data not shown) ...
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bioRxiv - Microbiology 2023Quote: ... for raw greywater samples and MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, product code MC85200) for treated greywater samples ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis and strand-specific library generation was carried out using the Script-Seq V2 Kit (Epicentre) and the FailSafe PCR enzyme mix ...
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bioRxiv - Microbiology 2023Quote: ... ribosomal RNA was removed using the Ribo-Zero rRNA removal kit for Gram-negative bacteria (Epicentre/Illumina), and libraries were prepared with the ScriptSeq Complete kit for bacteria (Epicentre/Illumina ...
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bioRxiv - Microbiology 2023Quote: ... rRNA depletion from total RNA was performed using the Ribo-Zero magnetic kit (Epicentre Biotechnologies, United States) and the mRNA was chemically fragmented to short pieces (200 nt ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted by using the MasterPure complete DNA and RNA purification kit (Epicentre, Madison, WI) as described by the manufacturer ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from cell pellets using the MasterPure™ Yeast DNA Purification Kit (Lucigen, cat #MPY80200), with an additional initial incubation with zymolyase at 37°C to enhance cell lysis ...