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Citations for Agilent :
601 - 650 of 1345 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... SDM PCRs were performed using Pfu Turbo DNA Polymerase (Agilent) followed by DpnI digest (NEB ...
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bioRxiv - Microbiology 2022Quote: ... All PCR were done using high-fidelity DNA polymerases (Agilent). The primers used in this study are listed in Table S2 ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR products were treated with DpnI (0.8 μl; Agilent) for 1 h at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... and the PCR products were assessed on a BioAnalyzer (Agilent) then sequenced (1×50 nt ...
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bioRxiv - Plant Biology 2023Quote: ... with a AriaMx real-time PCR system (Agilent Technologies, USA). DNA contents of each sample was determined in duplicates ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... A one-step qRT-PCR kit with SYBR green (Agilent) was used with the primer sequences below ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... or LRR residues were introduced using Quikchange Lightning PCR (Agilent). Ectodomain truncations were constructed using PCR and NEBuilder HiFi DNA Assembly ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed on AriaMX Real-Time (Agilent) in a final volume of 20µl ...
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bioRxiv - Neuroscience 2023Quote: ... and the Mx3000P Real-time quantitative PCR system (Agilent Technologies) or the CFX96™ Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were verified by the 2100 Bioanalyzer (Agilent, USA). The GenoToul platform (Toulouse ...
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bioRxiv - Immunology 2023Quote: ... using qRT-PCR Brilliant II Probe Master Mix (Agilent Technologies) with a TaqMan™ TAMRA Probe system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... and placed into an AriaMx Real-time PCR System (Agilent). Each run was initiated with a hot start at 95 °C for 15 minutes followed by 50 cycles consisting of a 15s denaturation step at 95 °C followed by a 60s amplification step at 60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR Brilliant III ultra-fast master mix kit (Agilent) and TaqMan gene expression probes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reactions were conducted using a RoboCycler Gradient 40 (Stratagene). Reaction solutions were incubated at 94°C for 5 minutes before undergoing 35-40 rounds of amplification cycles ...
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bioRxiv - Microbiology 2023Quote: ... Purified PCR products were analyzed on a Bioanalyzer (Agilent Technologies) and quantified on a Qubit 4 fluorometer (Invitrogen) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... PCR product size was analyzed with TAPE Station from Agilent. Clones with successful deletions based on the PCR result were then analyzed using Western blots with antibodies against FOS or EGR1 and pERK antibody (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2024Quote: ... with the AriaMx Real-Time PCR system (Agilent Technologies, USA). Expression levels of all tested genes were normalized to the house-keeping gene Ubiquitin 5 (UBQ5) ...
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bioRxiv - Neuroscience 2020Quote: ... SYBR Green method was used to perform quantitative Real-time PCR (qRT-PCR) with Brilliant-III ultrafast SYBR Green qPCR master mix (Agilent Technologies, Santa Clara, CA, USA) as per manufacturer’s protocol on an Agilent MX3000P ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed in TBST (3 × 15 mins at RT) and then incubated with corresponding peroxidase-conjugated secondary antibodies (Dako, P0447, P0448) for 1h at RT ...
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bioRxiv - Biochemistry 2019Quote: ... Each RT-qPCR reaction (20 μl final volume) contained 10 μl Brilliant III SYBR Green qPCR Master Mix (Agilent Technologies, Waldbronn, Germany), 0.6 μl of cDNA ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed 1 h at RT: anti-rabbit-IgG conjugated to horseradish peroxidase (1:10,000, cat. no. P044801-2; Dako, Carpinteria, CA, USA), anti-mouse-IgG conjugated to horseradish peroxidase (1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... Bacteria were fixed with 0.5% formaldehyde at RT for 30 min and then wells were washed twice with a washer dispenser (BioTek EL406, Agilent Technologies, US) with PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The mutated variants of AopW1 were generated by inserting mutations in the corresponding primers in combination with the Quick Change Lightening Site Directed Mutagenesis kit (Agilent Technologies, SC, USA). For this purpose ...
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bioRxiv - Physiology 2022Quote: ... The AccuScript Hi-Fi Kit and Oligo(dT) primers (18mers) were used for cDNA synthesis following the manufacturer’s instructions and using concentrations required (Agilent Technologies, Santa Clara, USA). An RNA digestion step was added using the enzyme RNAse H (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and the quality as well as yield of fully-barcoded DNA primers was evaluated with total RNA Pico Assay (Agilent Technologies, 5067-1513) and by fluorescent in situ hybridization (FISH ...
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bioRxiv - Plant Biology 2019Quote: ... were introduced by the Quick-Change PCR mutagenesis kit protocol (Stratagene), using the primer combinations listed in Supplemental Table 2 and PhusionTM High-Fidelity DNA Polymerase (Finnzymes) ...
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bioRxiv - Microbiology 2019Quote: ... The PCR product was analyzed on Agilent 2100 Bioanalyzer (Agilent Technologies) and gel purified using GeneJET Gel Extraction Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR mutagenesis was performed using the QuikChange Lightning enzyme (Agilent Technologies) at an annealing temperature of 60 °C for 18 cycles with the following components ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR-amplified and washed again before analysis on a Bioanalyzer (Agilent) for quality control ...
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bioRxiv - Microbiology 2020Quote: ... and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies), with specific primers ...
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bioRxiv - Biochemistry 2020Quote: ... Quantitative PCR was performed using a MxPro – Mx3005P thermocycler (Agilent Technologies) with Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: The library was created by error-prone PCR (Mutazyme II, Stratagene) following the manufacturer’s instructions with 50 ng of dam methylated NBI gene PCR product and 30 PCR cycles ...
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bioRxiv - Cancer Biology 2020Quote: ... the PCR fragments were cloned into pAAV-MCS (VPK-410, Stratagene) to construct the pAAV-DCUg-NT/hFL/mFL and pAAV-DMNeg/DMhFL/DMmFL vectors ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative PCR was performed by using QuantiNova kit on Mx3500P (Stratagene). The oligonucleotide primers used in this study are shown in Supplementary Table 2.
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bioRxiv - Cell Biology 2021Quote: ... amplified by PCR using Pfu Ultra HF DNA polymerase (Agilent Technologies) and pEGFP-C2-BICD2 as a template ...
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bioRxiv - Neuroscience 2020Quote: ... error-prone PCR was performed with the GeneMorphII kit (Agilent Technologies) or Taq polymerase (New England Biolabs ...
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bioRxiv - Systems Biology 2020Quote: ... block 3-6: PCR amplification from the synthetic library (Agilent Technologies) according to mutation families) ...
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bioRxiv - Immunology 2022Quote: ... on AriaMx Real-time PCR System (Agilent Technologies, Santa Clara, CA). We normalized the results to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) ...
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bioRxiv - Microbiology 2022Quote: ... A one-step qRT-PCR kit with SYBR green from Agilent was used ...
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bioRxiv - Microbiology 2022Quote: ... vector pDFTT3-CTL0481 was PCR-amplified (QuikChange II XL Kit, Agilent) using primers pDFTT3-rvcat_F and pDFTT3-rvcat_R (Table S2) ...
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bioRxiv - Immunology 2022Quote: ... on AriaMx Real-time PCR System (Agilent Technologies, Santa Clara, CA). We normalized the results to Actb (β-actin gene) ...
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bioRxiv - Immunology 2020Quote: ... Cycling was performed in a Mx3005P real time PCR machine (Stratagene) and included an initial 3 min enzyme activation step at 95°C ...
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bioRxiv - Microbiology 2020Quote: ... Amplified PCR products were analysed on a 2100 bioanalyzer system (Agilent) using a High Sensitivity DNA chip according to the manufactures guidelines ...
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bioRxiv - Cancer Biology 2020Quote: PCR reactions were performed using Quik-Change XL kit by Agilent Technologies (catalogue no.200517-4 ...
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bioRxiv - Genetics 2022Quote: ... PCR amplicons were sized on a fragment analyzer (Model# 5200; Agilent) using 600mer DNA separation gel (Cat# NDF-915-0275 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were cloned into pBluescript II-SK (pBS, Stratagene). Sequences were determined using the ABI 3130 Genetic Analyzer (Applied Biosystems).
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bioRxiv - Synthetic Biology 2020Quote: ... digested vectors or PCRs amplified with Herculase II fusion (Agilent# 600675). A portion of pENTR-D-TOPO (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: For qRT-PCR we used either the Stratagene Mx3005P (Agilent Technologies) or QuantStudio 7 Pro SmartStart (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative real–time PCR reagents were from Thermo Fisher Scientific and real-time PCR was performed using the Mx3000 (Stratagene) detection system ...
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bioRxiv - Biochemistry 2021Quote: Single-point mutations and deletions were generated using PCR mutagenesis (Agilent). Gibson assembly (NEB ...