Labshake search
Citations for Agilent :
651 - 700 of 1342 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... PCR reactions were performed with Herculase II Fusion DNA Polymerase (Agilent) in technical replicates with 50ng enriched mtDNA as template per reaction ...
-
bioRxiv - Genetics 2020Quote: ... The PCRs products were direct sequenced or subcloned into pBlueScript (Stratagene) followed by Sanger sequencing ...
-
bioRxiv - Molecular Biology 2022Quote: ... Quantitative real–time PCR reagents were from Thermo Fisher Scientific and real-time PCR was performed using a Mx3000 (Stratagene) and qTower3G (Analytik Jena) ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR amplicon was cloned in the pGEMT-easy plasmid (Stratagene) for further use ...
-
Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: ... PCR-amplified and washed again before analysis on a Bioanalyzer (Agilent) for quality control ...
-
bioRxiv - Microbiology 2023Quote: ... Clean PCR products were diluted and subjected to 2100 Bioanalyzer (Agilent) analysis following the manufacturer’s protocol to get the molar quantities of the two different-size amplicons ...
-
bioRxiv - Physiology 2022Quote: ... using an AriaMX realtime PCR machine (Agilent Technologies, Santa Clara, CA). Quantified libraries were pooled and sequenced on an Illumina NextSeq using a high capacity 75 read flow cell (Illumina ...
-
bioRxiv - Biochemistry 2023Quote: ... through site-directed PCR mutagenesis (PfuUltra High-Fidelity DNA Polymerase, Agilent). The resulting mutant DNAs were sequence verified ...
-
bioRxiv - Biophysics 2023Quote: ... PCRs were performed using high-fidelity Pfu ultra II polymerase (Agilent). All constructs and mutations were confirmed by DNA sequencing.
-
bioRxiv - Immunology 2023Quote: ... SIVmac239 molecular clone derivative was generated by mutagenesis PCR (Agilent Technologies) using the primers listed ...
-
bioRxiv - Biochemistry 2023Quote: ... Site-directed mutations were introduced by PCR (Quikchange II, Agilent Technologies). Cultures were grown in Lemo21 (DE3 ...
-
bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed in Stratagene Mx3005p qPCR system (Agilent Technologies) using TB green pre-mix Ex-Taq II (Cat#RR208 ...
-
bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed using the Mx3000P QPCR System (Agilent Technologies Inc. ...
-
bioRxiv - Zoology 2024Quote: ... Amplification was conducted on AriaMax Real-Time PCR system (Agilent, France) and consisted of an initial RT step at 42°C for 20 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fluorescence was measured in a real-time PCR machine (Stratagene Mx3005P) while subjected to a temperature gradient from 24.6°C to 95°C in steps of 1°C and 1 min.
-
bioRxiv - Biophysics 2023Quote: ... Fluorescence was monitored using an Mx3005P real-time PCR instrument (Stratagene) with excitation and emission filters set to 465 and 590 nm ...
-
bioRxiv - Neuroscience 2023Quote: ... fluorescence was evaluated using a Real-Time PCR Mx3005p machine (Stratagene). A previously reported protocol was followed to run the Tm shift assays and assess melting temperatures[51] ...
-
bioRxiv - Developmental Biology 2023Quote: ... and Agilent AriaMx Real-Time PCR system (Agilent, Santa Clara, USA), according to manufacturer’s guidelines ...
-
bioRxiv - Genetics 2023Quote: ... we cloned pegRNA libraries with PCR tags (ordered from Agilent Technologies) into the lentiviral vector SgOpti (Addgene #85681) ...
-
bioRxiv - Genomics 2020Quote: ... Final library quality control consisted of confirmation of amplification and barcoding by SYBR Green-based RT-qPCR (Stratagene Mx3005P QPCR System, Agilent, Santa Clara, CA), visualization on a 2% agarose E-Gel (Invitrogen ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... the coverslips were incubated in donkey anti goat-647 for TRKB and donkey anti rabbit-568 for the RAB-specific antibodies for 45 min at RT and fixed in Dako Fluorescence Mounting Medium (S3023, Dako North America, Inc.). Control coverslips without primary antibody staining were also included.
-
bioRxiv - Neuroscience 2022Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
-
bioRxiv - Bioengineering 2022Quote: ... eye cups were post-fixed for 10 min in 4 % FA-PBS at RT following by flat-mounting with fluorescent mounting medium (Dako, Carpinteria, CA, USA). Fluorescence images were acquired with Nikon Eclipse Ti-E microscope (Nikon ...
-
bioRxiv - Cancer Biology 2023Quote: ... and hypoxanthine guanine phosphoribosyl transferase (HPRT) were assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR, Mx3005P QPCR System (Agilent, Santa Clara, CA). After 24 or 48 h of incubation in normoxia ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were incubated for 1h at RT before overnight incubation at 4°C with primary antibodies: Glial Fibrillary Acidic Protein (GFAP) (1/500 Dako Cat. Number Z0334); AQP4 (1/500 ...
-
bioRxiv - Neuroscience 2024Quote: ... and then incubated 1 hour at room temperature (RT) with EnVision HRP system anti-mouse (K400111-2, Agilent Technologies, Santa Clara, CA, USA) in blocking solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... the samples were amplified in duplication using SYBR Green (Vazyme, Q431-02) with 200 nM of gene-specific primers and run on the CFX amplifier (MX3000P, Stratagene, La Jolla, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... In the 1st step YeGFP (referred to as GFP) was amplified using GFP specific primers and Mutazyme II polymerase (Agilent Technologies; Cat no. 200550) in order to incorporate 7-11 mutations per kb of plasmid ...
-
bioRxiv - Physiology 2021Quote: ... was amplified by PCR using cDNA of mouse kidney at the template with the primers 5′-CCGTCGACATGCAGGTCAAGAAATCCTG-3′ (SalI site in italics) and 5′-CCGGATCCTTAAAGGCGTGTGTGATCTT-3′ (M6 reverse primer; BamHI site in italics) and cloned into pBluescript SK(−) (Stratagene, La Jolla, CA, USA) using the SalI and BamHI sites ...
-
bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
-
bioRxiv - Physiology 2020Quote: ... Real-time PCR amplification with the 2 x precision master mix (Primer design, Southampton, UK) using the Stratagene Mx3000P real-time QPCR system (Agilent Technologies, Santa Clara, CA, USA). Each sample was tested in triplicate and compared to a housekeeping gene (Atp5B in osteoblasts and bone tissue ...
-
bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
-
bioRxiv - Cell Biology 2020Quote: ... PCR mutagenesis was performed using QuickChange II site directed mutagenesis kit (Agilent) according to the manufacturer’s protocol.
-
bioRxiv - Genomics 2020Quote: ... pre-capture PCR and SureSelectXT Human All Exon V5+UTR baits (Agilent). Post-capture fragments were amplified by PCR ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was conducted using the Stratagene Mx3005P detection system (Agilent Technologies) and GoTaq qPCR mix (Promega) ...
-
bioRxiv - Developmental Biology 2022Quote: ... PCR products were cloned into pSC-A-amp/kan Strataclone plasmids (Agilent) to generate probes for wholemount in situ hybridization experiments (Table S1) ...
-
bioRxiv - Microbiology 2019Quote: ... For this experiment Brilliant III ultra-fast qRT-PCR master mix (Agilent) was used with ROX following manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... to generate pScGFPTUB1 using a Stratagene blunt PCR cloning kit (Agilent Technologies). The neomycin (NEO ...
-
bioRxiv - Microbiology 2021Quote: ... For replicates 1 and 2 a Strategene Mx3005P PCR machine (Agilent, USA) was used ...
-
bioRxiv - Cell Biology 2021Quote: ... on an AriaMx Realtime PCR system (Agilent Genomics, Santa Clara, CA, USA). Results were normalized to TBP and RPL13A housekeeping gene expression levels ...
-
bioRxiv - Plant Biology 2021Quote: ... and the AriaMx Real-Time PCR system (Agilent Technologies, Santa Clara, USA). The house keeping gene UBQ was used as a reference gene using primers qUBQ_F and qUBQ_R ...
-
bioRxiv - Microbiology 2020Quote: ... Mutagenesis was performed by PCR using the Quikchange mutagenesis kit (Agilent Technologies) and primers specified in Table S2 ...
-
bioRxiv - Cancer Biology 2022Quote: ... The PCR was performed using Herculase II Fusion DNA Polymerase (Agilent Technologies) and primers are listed in Table S5 ...
-
bioRxiv - Molecular Biology 2020Quote: ... All quantitative PCR amplifications were run with Mx3000P QPCR System (Agilent, Germany). All primers and probes were ordered from Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... PCR was performed using PfuUltra II Fusion HS DNA Polymerase (Agilent Technologies) and primers 5′-TGACACTACTGATGCTGTCCGTG-3′ and 5′-GATGGATCTGGTAATATTTGTG-3′ under the following conditions ...
-
bioRxiv - Microbiology 2021Quote: ... using an AriaMx Real-Time PCR system (Agilent, Santa Clara, CA, USA). The relative quantification of the target mRNA levels was performed using the 2-ΔΔCT method ...
-
bioRxiv - Biophysics 2019Quote: ... The PCR reaction was cycled using the parameters as described by Agilent. XL10-Gold cells were then transformed with the PCR reaction per Agilent’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... The resulting PCR product was quality controlled on a 4200 TapeStation (Agilent), purified using Agencourt AMPure XP (Beckman Coulter ...
-
bioRxiv - Immunology 2019Quote: ... Quantitative real-time PCR was performed using SYBR green mix (Agilent Technologies) and a LightCycler 480 II (Roche) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and genetically authenticated using a STR-PCR fragments kit (Agilent Technologies, USA) as per our previous published technique [37] ...