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Citations for Agilent :
301 - 350 of 1343 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... annealed with gene-specific primer and reverse-transcribed using AffinityScript Multiple Temperature Reverse Transcriptase (#600107, Agilent) with following conditions ...
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bioRxiv - Microbiology 2020Quote: ... annealed with gene-specific primer and reverse-transcribed using AffinityScript Multiple Temperature Reverse Transcriptase (#600107, Agilent) with following conditions ...
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bioRxiv - Plant Biology 2020Quote: ... and the primers (SLR1t-STOP-Fw and SLR1t-STOP-Rv) designed with QuickChangeII online tool (Agilent) (Supplemental Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: ... with a specific primer (5’-CCTACACGACGCTCTTCC-3’) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). Then ...
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bioRxiv - Microbiology 2022Quote: ... primers were designed according to the protocol of the QuickChange II Site-directed mutagenesis kit (Agilent) and as listed in table S2 ...
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bioRxiv - Microbiology 2022Quote: ... and reverse transcribed using random primers with the AffinityScript multiple temperature reverse transcriptase (Agilent, Wilmington, DE). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 μM of each primer and 2.5 U of PfuTurbo Cx Hotstart DNA Polymerase (Agilent Technologies). Following completion of mutagenesis reactions ...
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bioRxiv - Biochemistry 2023Quote: ... Mutagenesis of the plasmids was performed with the indicated primers (Table S4) using PfuTurbo Polymerase (Agilent) followed by DpnI (NEB ...
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bioRxiv - Cell Biology 2020Quote: Quantitative reverse transcription PCR (qRT-PCR) analysis was carried out on an MX300-P (Agilent technologies) using gene specific primers (QuantiTect® ...
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bioRxiv - Biochemistry 2023Quote: ... SYBR Green Real-time PCR experiments were performed using AriaMx Real-Time PCR (Agilent, California, America) and Taq Pro Universal SYBR qPCR Master Mix (Vazyme) ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed on a Mx3000P real-time PCR system (Stratagene, San Diego, CA, USA) using SYBR® Premix Ex Taq™ II (TaKaRa) ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... PCR was performed using Mx3005P (Agilent) with SYBR® Premix EX TaqTM II (Takara ...
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bioRxiv - Genetics 2021Quote: ... and 5081) were used for whole-exome sequencing prepared using the SureSelect Human All Exon V7 probe set (Agilent, Design ID S31285117) per the manufacturers protocol ...
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... One set of single and mixed samples was stained with a FITC PNA probe and the other set was left unstained to correct for auto-fluorescence (Dako telomere kit). DNA was denatured for 10 min at 82°C and allowed to hybridize overnight ...
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bioRxiv - Synthetic Biology 2021Quote: 384 well qPCR reaction plates for the multiplexed activation of endogenous genes (Figure 5 and 6) were set up using the Brilliant II SYBR master mix (Agilent cat #600828) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... and enriched the libraries for ∼2.34 mB of the MSY via in-solution hybridization-capture using a previously-designed probe set (Kutanan et al. 2018b) and the Agilent Sure Select system (Agilent, CA, USA); further details on the probe design are provided in Table S6 ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were air-dried at RT for 30 min and a hydrophobic pen (DAKO, Cat# S2002) was used to delineate the border of the slide surface ...
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bioRxiv - Genomics 2020Quote: ... After incubation with HRP-conjugated secondary antibodies (1:5000, DAKO p0260 and p0217, 1h at RT), bands or dots were imaged on a chemiluminescence detection system (Bio-rad).
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bioRxiv - Plant Biology 2020Quote: ... prior to the first-strand cDNA synthesis using AffinityScript RT-qPCR cDNA synthesis kit (Agilent Technologies). RT-qPCR analysis was conducted using iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR using Brilliant II SYBR® Green QPCR Master Mix (Agilent, Santa Clara, CA, USA) was outperformed on CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... the tissue sections were blocked for 1 h at RT with protein blocking solution (#X0909, Dako). After washing with PBS the aortic tissue sections were specifically stained with primary antibodies for platelet GPIbα (CD42b #M042-0 ...
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bioRxiv - Cell Biology 2022Quote: ... The UAS site deletion was generated performing single primer mutagenesis using Pfu Turbo Cx DNA polymerase (Agilent). The pBID-GmCherry vector was constructed by replacing the mVenus of the pBID-GV vector by the mCherry sequence ...
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bioRxiv - Microbiology 2022Quote: ... 200 nM of each primer and 1X Brilliant III Ultra-Fast SybrGreen qPCR Master Mix (Agilent Technologies). The RT-qPCR reactions were performed on a QIAGEN Rotor-Gene Q instrument (Corbett Research ...
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bioRxiv - Neuroscience 2022Quote: ... The N100A zDHHC/A17 mutants were generated by our laboratory by site-directed mutagenesis using primers designed by Agilent QuickChange II XL primer design software and using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies), and confirmed by DNA sequencing through Eurofins Genomics (Louisville ...
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bioRxiv - Molecular Biology 2019Quote: ... Radioactive probes were synthesized using a Prime-It II Random Primer Labeling Kit (300385, Agilent Technologies, Inc).
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bioRxiv - Molecular Biology 2020Quote: ... Oligonucleotides with the desired mutations were designed using web-based programs (Agilent Primer Design Program or NEBaseChanger) and purchased from Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... and the absence of primer dimers verified on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies). For sequencing a MiSeq V2 300 cycles kit (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... The immobilized Hi-C libraries were amplified using the SureSelectXT ILM Indexing pre-capture primers (Agilent Technologies) with eight PCR amplification cycles ...
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bioRxiv - Microbiology 2021Quote: ... berghei gDNA using primers 9483/9484 and 9485/9486 respectively and introduced sequentially into pBSKS+ plasmid (Stratagene) at XhoI/BamHI and EcoRI/SacII restriction sites respectively ...
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bioRxiv - Plant Biology 2021Quote: ... a site-directed mutagenesis using primers RACB_D121N_fw and RACB_D121N_rv was performed according to QuikChange® Site-Directed Mutaganesis Protocol (Stratagene).
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bioRxiv - Biochemistry 2022Quote: ... which was labelled with alpha-32P-ATP using the Prime-It II Random Primer Labelling Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Probes were labeled with 32P using the Prime-It II Random Primer Labeling Kit (Agilent Cat. #300385).
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bioRxiv - Biophysics 2023Quote: ... The WT Notch construct was then mutated using designed primers and Quik Change Lightning mutagenesis kit (Agilent) according to the manufacturer’s instruction to give Notch_L36F construct ...
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bioRxiv - Molecular Biology 2023Quote: ... QuickChange primers as well as the QuickChange II site-directed mutagenesis kit (Agilent Technologies, Santa Clara, USA) were used to incorporate point mutations into human GAT1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on a Agilent AriaMX Real-Time PCR System (Agilent Technologies, Santa Clara, CA) and master mixes for each qRT-PCR reaction used PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-time PCRs were carried out in Agilent AriaMx Real-time PCR System (Agilent technologies, CA, US). The comparative ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Microbiology 2020Quote: ... Each real-time PCR was performed in triplicate on the Stratagene Mx3005P real-time PCR system (Agilent). The reaction mixture was incubated for 15 min at 95°C ...
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bioRxiv - Genetics 2024Quote: ... The quantitative PCR (qPCR) amplification was carried out in a MX 3005 real-time PCR system (Agilent) using Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned using StrataClone PCR cloning kit (product no: 240205, Agilent technologies Sweden AB, Kista) and re-amplified with M13F/M13R primers using DreamtTaq (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were ligated into pSC-A vectors using a Blunt-end PCR cloning kit (Agilent #240207). Ligated plasmids were transformed into XL-1 blue competent cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (5 ng cDNA/well) was performed by using QuantiNova kit on Mx3500P PCR machine (Stratagene). Gene-specific primers were used for end-point PCR (HotStarTaq Plus DNA Polymerase ...
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bioRxiv - Neuroscience 2022Quote: ... Amplified cDNAs for a set of random selected samples were quantified and run on a High Sensitivity Bioanalyzer chip (Agilent, cat# 5067-4626) on a 2100 Bioanalyzer (Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... The slides were processed as described and probed with a set of 258 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... Optical density of each sample was read at 450 nm with a wavelength correction set to 540 nm using a BioTek Gen5 Microplate Reader and Imager Software (Agilent, Santa Clara, CA). The triplicate OD measurements for each standard and tissue sample were averaged and the BDNF concentration in pg/ml was calculated and converted to pg/mg tissue weight.
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bioRxiv - Cancer Biology 2024Quote: ... The slides were processed as described and probed with a set of 264 antibodies against total proteins and phosphoproteins using an automated slide stainer Autolink 48 (Dako, Santa Clara, CA). Each slide was incubated with one specific primary antibody and a negative control slide was incubated with antibody diluent without any primary antibody ...
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Adolescent parvalbumin expression in the left orbitofrontal cortex shapes sociability in female micebioRxiv - Animal Behavior and Cognition 2023Quote: ... for 2 hours at RT before mounting the slices in a transparent mounting medium (DAKO, cat#S3023). We manually and blindly counted the number of WFA+ neurons within 0.5 × 0.5mm2 ROIs in three representative coronal sections covering OFC.
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bioRxiv - Cell Biology 2023Quote: ... RT–qPCR reactions were performed using Brilliant III Ultra-Fast SYBR Green qPCR Master mix (Agilent Technologies) with the relevant primers (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was carried out using Brilliant III Ultra-Fast SYBR Green qPCR kit (Agilent Technologies) on an AriaMx G8830A Real-Time qPCR System (Agilent Technologies) ...
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bioRxiv - Cell Biology 2023Quote: RT-qPCR was performed by using the Brilliant III SYBR® Green QPCR Master Mix (Agilent #600882) or DyNAmo HS SYBR Green (Thermo Scientific #F410L ...
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bioRxiv - Cancer Biology 2024Quote: ... then long-term drug responses were recorded every 30 minutes through (RT-CES) (ACEA Biosciences; Agilent Technologies). Cell Index values (CI ...