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Citations for Agilent :
151 - 200 of 733 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: The 3a ORF from SARS CoV-1 (Tor2 strain) was originally obtained from the Institute for Genomic Research and subcloned into pBluescript (Stratagene) prior to transferring to pCAGGS-MCS [17] after PCR amplification with KpnI and XhoI restriction sites ...
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Plant Biology 2021Quote: ... and cloned into pVp16-Dest vector for IPTG (isopropyl-β-thiogalactopyranoside)-induced expression (3 h at 37°C) in E.coli strain ArcticExpress (Agilent). After sonication ...
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bioRxiv - Genomics 2021Quote: The different Sen1 HD versions were fused to a C-terminal His8-tag coupled to Vibrio cholerae MARTX toxin cysteine protease domain (61)and expressed from the T7 promoter (see plasmids in table S6) in Escherichia coli strain BL21 (DE3) CodonPlus (Stratagene). Overexpression was induced by growth in auto-inducing medium (62 ...
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bioRxiv - Biophysics 2020Quote: A Pol I expression vector was generated from the plasmid pXS67 (Yale Coli Genetic Stock Center, Strain CJ803) by site-directed mutagenesis using a QuikChange kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The obtained amplicons were blunt-cloned into an EcoRV-digested pBluscript KS+ plasmid and transformed into the Sure Escherichia coli strain (Stratagene), cultured at 30 °C.
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bioRxiv - Microbiology 2022Quote: ... SINV strain SVIA equivalent to MOI of 500 was irradiated for 7 minutes using a Stratalinker UV Crosslinker 1800 (Stratagene). Absence of infectious particles was confirmed through plaque assay on Vero cells ...
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bioRxiv - Plant Biology 2022Quote: Plasmids were transformed and expressed in the Escherichia coli strain BL-21 (DE3) RIL cells (Agilent, Santa Clara, CA, USA). For protein expressions ...
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bioRxiv - Microbiology 2023Quote: ... The growth rate of strains encoding Omp33 site directed mutants was measured in the BioTek LogPhase 600 Microbiology Reader (Agilent) continuously every 10 mins for 24h at 37°C with shaking at 500 RPM.
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bioRxiv - Cell Biology 2023Quote: Genes of interest were PCR cloned from either cDNA or gDNA of N2 Bristol strain using PfuUltra II Fusion HS DNA polymerase (Agilent). Amplicon was ligated into pSM vector backbone for expression in C ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: HIV-1 Pol constructs of the BH10 strain (gift from Michael Parniak) were cloned into a pET28a vector and transformed into BL21-CodonPlus (DE3)-RIL cells (Agilent Technologies) alone or co-transformed with MBP-IBD ...
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bioRxiv - Microbiology 2020Quote: His-tagged VP35 IID (VP35 aa 215-340) and GST-NP (aa 412-500) or shorter variants were expressed in the BL21 CodonPlus strain (Agilent Technologies). Cells were lysed in Buffer (0.15 ...
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bioRxiv - Genetics 2019Quote: The Ycp50-rad50-L1240F plasmid was randomly mutagenized by propagating the plasmid in XL1 red bacterial mutator (mutS, mutD, mutT) strain (Agilent Technologies). rad50Δ mec1Δ sml1Δ sae2Δ spore (JPY1953 ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Cancer Biology 2020Quote: ... dry protein A cartridges (Agilent) were primed in PBS at 300 μL/min before loading 1 mg of antibody at 1 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Protein block (DAKO) for 10min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... BSA and Protein block (Dako). Sections were stained with primary antibodies against GFP (ab290 ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant transfer vector was linearized by PmeI and transformed into electro-competent E.coli strain BJ5183-AD-1 (Stratagene, Cat. No. 200157-11) for in vivo recombination with pAdEasy vector ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein Block Serum Free reagent (Dako). Primary antibody incubation was performed at 4°C overnight using the ideal dilution for each antibody (Supplementary Table 5) ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated in Protein Block (Dako) for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and protein (Dako cat. no. X0909) blocking reagents for 5 minutes each ...
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bioRxiv - Microbiology 2023Quote: ... AdvanceBio SEC 300Å protein standard (Agilent) was included to correlate the elution volume with molecular weight.
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bioRxiv - Microbiology 2023Quote: ... diluted with protein block buffer (Dako) for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... protein blocker (Agilent Technology, X090930-2). Slides were incubated with a biotinylated anti-pimonidazole mouse IgG1 monoclonal antibody diluted 1:50 in Background Sniper (Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Dako Protein Block (Agilent Cat#X0909), ‘Normal’ block (Agilent Cat#S202386-2) ...
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bioRxiv - Genomics 2020Quote: ... The constructed expression vector named pET28a-SUMO-MyoD-FL was transformed into the Escherichia coli strain BL21 (DE3) (Agilent Technologies, Santa Clara, CA, USA). The cells were grown in LB medium supplemented with 50 mg/ml kanamycin at 37°C until OD600 reached 0.6-0.8 ...
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bioRxiv - Microbiology 2021Quote: The carotenoids produced by strain LZ-28 were extracted and determined as described previously [21] by an Agilent 1200 high performance liquid chromatography (Agilent, Palo Alto, CA, USA) equipped with a diode-array detector (DAD) ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP-tagged Cdc122FH1-Fus1FH2 and Cdc122FH1-Fus1R1054EFH2 constructs (including the C-terminal tail) were expressed in Escherichia coli strain BL21-Codon Plus (DE3) RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Immunology 2020Quote: ... proteins were tetramerized with streptavidin-APC (Prozyme) or streptavidin-AlexaFluor488 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Proteins were tetramerized with streptavidin-APC (Prozyme), streptavidin–Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Additional protein block from Dako (X090930-2) was applied ...
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bioRxiv - Neuroscience 2023Quote: ... blocked (30 min, protein block X0909, Dako), and stained overnight at 4°C using primary antibodies detecting F4/80 ...
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bioRxiv - Immunology 2023Quote: ... using the Protein G AssayMAP Bravo (Agilent) system ...
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bioRxiv - Immunology 2023Quote: ... using the Protein G AssayMAP Bravo (Agilent) system ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Cancer Biology 2021Quote: ... and an additional serum-free protein block (DakoCytomation) to reduce nonspecific binding of endogenous proteins ...
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bioRxiv - Synthetic Biology 2019Quote: ... After blocking with serum-free protein block (Dako), they were incubated with the primary antibodies for 120 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-glial acidic fibrillary protein (GFAP)(Dako), mouse anti-glutamic acid decarboxylase 67 (GAD67 ...
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bioRxiv - Bioengineering 2020Quote: Proteins were expressed in BL21(DE3)Gold (Stratagene) and purified as described previously6 ...
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bioRxiv - Biophysics 2021Quote: ... proteins were expressed using BL21(DE3) cells (Agilent). The cells were grown at 37°C until an optical density at 600nm of .6 then induced with 0.2mM isopropyl-Beta-D-thiogalactoside overnight at 18° C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein blocking was performed for 20 min (Dako). NUP210 primary antibody (Atlas ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was overexpressed in BL21(DE3) RIL (Agilent) at 37 °C in Terrific Broth (TB ...
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bioRxiv - Pathology 2021Quote: ... Slides were placed in a Protein Block (DAKO) for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and Protein-Block solution (DAKO Agilent technologies, X0909) respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... and Protein-Block solution (DAKO Agilent technologies, X0909) respectively ...