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Citations for Agilent :
151 - 200 of 5433 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Samples were labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... The full construct was inserted into the multiple cloning site of pBluescript II SK (+) (Stratagene).
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bioRxiv - Developmental Biology 2022Quote: ... Amplified fragments were then cloned into StrataClone PCR Cloning Vector pSC-A-amp/kan (Stratagene) and sequenced in order to check for the identity and orientation of the inserted PCR fragment ...
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bioRxiv - Microbiology 2021Quote: All transformations for cloning or plasmid amplification were performed in Escherichia coli XL10 Gold (Agilent) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Relative expression was assessed by qPCR with SyberGreen II mix (Agilent Technologies Thermocycler ...
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bioRxiv - Cancer Biology 2020Quote: MiRNAs expression for 66 matched tumor and normal samples from Agilent platform were analyzed as described in Ganci et al ...
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bioRxiv - Cell Biology 2019Quote: ... using the Canis (V2) Gene Expression Microarray 4×44K (Agilent Technologies). Microarray data were subjected to gene ontology (GO ...
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bioRxiv - Biochemistry 2022Quote: ... Expression was performed in BL21(DE3)- RIPL Escherichia coli cells (Agilent) in LB at room temperature and induced through the addition of 0.2 mM IPTG for ~24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... The expression analysis was performed using the MxPro QPCR software (Agilent).
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bioRxiv - Molecular Biology 2023Quote: ... The expression plasmid was transformed into BL21-CodonPlus Competent Cells (Agilent), and protein expression was done by the addition of IPTG (1 mM final concentration ...
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bioRxiv - Genomics 2023Quote: ... elegans (V2) Gene Expression Microarray 4 × 44k chips were used (Agilent). Scanning was done with an Agilent High Resolution C Scanner ...
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bioRxiv - Cell Biology 2023Quote: ... The hybridized slides were washed using Gene Expression wash buffers (Agilent), and scanned using the Agilent Microarray Scanner (G2505C ...
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bioRxiv - Neuroscience 2021Quote: ... The library was created by cloning a pool of sgRNA-containing oligonucleotides custom-synthesized by Agilent Technologies into our optimized sgRNA expression vector as previously described81 ...
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bioRxiv - Biophysics 2019Quote: A single point mutation (G178D) was introduced using QuickChange site-directed mutagenesis (Stratagene Cloning Systems, CA) to both the wild type and K62W mutant truncated cKir2.2 channel cDNA and verified by sequencing ...
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bioRxiv - Microbiology 2022Quote: ... all clonings in the suicide vector pSW7848T were performed using Herculase II fusion DNA polymerase (Agilent) for PCR amplification and the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... The transposon junctions were amplified using a nested PCR protocol using Easy-A cloning enzyme (Agilent) in which the 3’-poly-dC DNA fragments were amplified ...
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bioRxiv - Plant Biology 2022Quote: ... The concentration of copper in processed yeast cells was analyzed by inductively coupled plasma mass spectroscopy (ICP-MS; Agilent 7500).
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bioRxiv - Microbiology 2023Quote: Yeast proteins under both conditions were extracted and later subjected to fractionation through 3100 OFFGEL (Agilent Technologies, Palo Alto, CA) followed by an identification by LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies, Lexington, MA) (34) ...
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bioRxiv - Immunology 2021Quote: ... Hybridization to SurePrint G3 Human Gene Expression 8×60K Microarrays (Agilent Technologies) was performed with the Gene Expression Hybridization Kit (Agilent Technologies).
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bioRxiv - Cancer Biology 2020Quote: ... cRNA were hybridized to SurePrint G3 Mouse Gene Expression 8×60K (Agilent) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was performed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Agilent). Bacterial cell cultures were grown in LB at 37°C to an OD600 of 0.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... median-centered pre-treatment expression data (assayed on custom Agilent 44K arrays). Signature genes were mapped to the dataset using gene symbol ...
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bioRxiv - Cancer Biology 2019Quote: ... Expression constructs were transformed into BL21-CodonPlus DE3-RIL bacteria (Agilent Technologies) for protein production ...
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bioRxiv - Physiology 2021Quote: ... Gene expression profiles (HTA 2.0 array) and plasma insulin values (K6219 Dako high-sensitivity enzyme linked immunosorbent assay (ELISA) ...
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bioRxiv - Genetics 2021Quote: ... the Agilent iGenex v.2 gene expression array (Agilent, Santa Clara, CA) is a custom-designed array with 31,344 probes targeting all exons of 2099 genes ...
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bioRxiv - Biochemistry 2021Quote: ... and Gene Expression Wash Buffer 2 (Agilent Technologies, Part Number 5188-5326) and were scanned using Agilent Scanner (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The hybridized slides were washed using gene expression wash buffer (Agilent, USA) and scanned ...
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bioRxiv - Systems Biology 2023Quote: ... Gene expression and guide libraries were subsequently quantified on the Bioanalyer (Agilent) using the High Sensitivity DNA kit ...
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bioRxiv - Biophysics 2023Quote: ... Bacterial protein expression was from BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies), unless otherwise stated ...
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bioRxiv - Neuroscience 2023Quote: ... RNA expression was measured using SYBR green on a QuantStudio 3 (Agilent). Probes were generated using NCBI primer blast and are listed in Table X ...
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bioRxiv - Physiology 2024Quote: ... Hybridization to SurePrint G3 Human Gene Expression 8×60K Microarrays (Agilent Technologies) was performed with the Gene Expression Hybridization Kit (Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Washing using Agilent Gene Expression wash buffers (Agilent Technologies, Part # 5188-5327) and scanning was done using the Agilent Microarray Scanner (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies, Lexington, MA) (61) ...
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bioRxiv - Physiology 2023Quote: ... Standard PCR reactions for cloning or site-mutagenesis were done using Herculase II Fusion polymerase (Agilent, Germany). All primers were ordered from Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: The pET-28a+ expression construct was transferred into BL21(DE3)/pLys strain (Stratagene) and protein production was induced at 37°C with 1 mM IPTG (isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Cell Biology 2019Quote: ... Following ‘One-Color Microarray Based Gene Expression Analysis’ protocol version 6.0 (Agilent Technologies), 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Bacteria for histone expression were BL21(DE3)pLysS and BL21(DE3) cells (Agilent). Transformed bacteria were grown on 2xTY media (1.6% Bacto Tryptone ...
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bioRxiv - Biochemistry 2021Quote: pMtac expression plasmids were transformed into BL21-Codon Plus RIPL competent cells (Agilent). Expression of His6-tagged proteins were induced at 16°C overnight with 0.25 mM IPTG ...
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bioRxiv - Genomics 2019Quote: ... miRNAs expression levels were analyzed using MXPro qPCR software 4.10 version (Stratagene, USA). Three biological replicates were examined to ensure reproducibility.
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bioRxiv - Microbiology 2020Quote: All PKR and viral genes were cloned into the pSG5 expression vector (Stratagene) for transient transfection assay ...
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bioRxiv - Physiology 2022Quote: ... We used a Mouse Gene Expression 4 × 44K Microarray chip (G4846A, Agilent Technologies), which can examine 23,215 genes as described [30] ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA expression was performed using Brilliant II SYBR Green QPCR Master Mix (Agilent) using a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... the slides were washed with Gene Expression Wash Buffers 1 and 2 (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the bacterial expression vector pLaG16 was transformed in Arctic Express (DE3) cells (Agilent). Bacteria were then induced with 0.1 mM IPTG for 16 hours at 8°C and centrifuged at 5,000 g for 10 minutes at 4°C ...
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bioRxiv - Genomics 2023Quote: ... The gene expression library and barcode library were evaluated using a TapeStation (Agilent) and qPCR before being pooled.
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bioRxiv - Neuroscience 2021Quote: The hydrophilic N-terminal region of CLN6 was screened against a human fetal brain library using Stratagene’s Cytotrap yeast two-hybrid system (Y2H) as previously published (Stratagene, La Jolla, CA). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... An inducible V-1 expression plasmid was created by first PCR- amplifying the mtpn gene with V-1 F and V-1 R oligos (dictyBase:DDB_G0268038) using Ax2 genomic DNA then TA cloning the product using StrataClone (Agilent) to generateV-1 SC ...
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bioRxiv - Physiology 2022Quote: ... slc15a2b was cloned into a StrataClone blunt PCR cloning vector pSC-B (Agilent Technologies, Palo Alto, CA, USA), while an additional step which added 3’ A-overhangs to the slc15a2a purified PCR product was performed using Taq DNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: General cloning was carried out in Escherichia coli DH5α or XL1-Blue (both Stratagene, La Jolla, CA, USA). All cloning techniques and the bacterial culturing were performed according to standard procedures (29) ...