Labshake search
Citations for Agilent :
251 - 300 of 5495 citations for WesternBright MCF Fluorescent Western Blot Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... HRP conjugated secondary antibodies used for Western blotting were anti-rabbit-HRP (0.25 μg/ml, goat polyclonal, Dako, P0448), anti-mouse-HRP (1.3 μg/ml ...
-
bioRxiv - Genomics 2022Quote: The following antibodies were used for western blotting or immunoprecipitation: rabbit IgG-HRP polyclonal antibody (PAP; code Z0113; Dako), rabbit Hsp60 polyclonal antibody (gift from J.P ...
-
bioRxiv - Molecular Biology 2021Quote: ... The dried blots were then crosslinked twice to the membrane using 120,000 µJ / cm2 on a Stratalinker UV crosslinker (Stratagene).
-
bioRxiv - Neuroscience 2019Quote: ... The blots were washed again and an alkaline-phosphate-conjugated goat anti mouse antibody was added (DAKO, 1:2000). Another washing step with TBST and B3 buffer (100 mM Tris ...
-
bioRxiv - Cell Biology 2021Quote: ... The aorta was opened in a reproducible manner by cutting along the greater curvature then mounted on glass slides with mounting medium (Dako Fluorescent; DakoCytomation).
-
bioRxiv - Cell Biology 2020Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2h at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated in 100 ng/mL DAPI for 10 minutes before mounting slides using the Fluorescent mounting medium (DAKO). Cells were visualized on Zeiss LSM780 confocal microscope using the ZEN software ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hours at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope.
-
bioRxiv - Molecular Biology 2021Quote: ... cells were then washed twice with DPBS and mounted using DAKO fluorescent mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI; Agilent). For visualisation of endogenous TDP-43 and FUS ...
-
bioRxiv - Microbiology 2021Quote: ... Nucleus staining was performed with DAPI 1 µg/mL for 1 min and coverslips were mounted on slides with fluorescent mounting medium (Dako). Cells were observed under a SP8 confocal laser-scanning microscope equipped with an HCP PL APO 100x/1.44 Oil CORR CS immersion objective (Leica) ...
-
bioRxiv - Bioengineering 2020Quote: ... for 20 minutes at room temperature before mounting with Dako Fluorescent mounting medium (catalog no. s3020; DAKO, Carpinteria, CA, USA). For histological assessment ...
-
bioRxiv - Cancer Biology 2019Quote: ... Devices were then washed with 1× PBS solution for 3 times and mounted on glass slides using fluorescent mounting medium (Dako) and the coverslips were sealed using nail polish ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The measurement of thermally induced release of these substances from the liposomes was based on repeated liposome separation by centrifugation and fluorescence emission intensity measurement of the supernatant (Cary Eclipse Fluorescent Spectrophotometer, Agilent). First ...
-
bioRxiv - Neuroscience 2021Quote: ... to test the presence of electrical coupling between THINs and NGF interneurons we used double transgenic TH–Cre crossed with (BAC) transgenic mice that express the humanized Renilla green fluorescent protein (hrGFP) (Stratagene) under the control of the mouse NPY promoter (NPY-GFP ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then all membranes were washed 3 times and mounted on coverslips using an aqueous fluorescent mounting medium (DakoCytomation, Huddinge, Sweden). Samples were analysed with Leica TCS SP8 confocal microscope (Leica Microsystems ...
-
bioRxiv - Cancer Biology 2022Quote: ... the tumor and tumoroid sections were mounted on the slide with Dako Fluorescent Mounting Medium (Agilent, Santa Clara CA, USA). Samples without the addition of primary antibody were used as negative control ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... OCI-AML3 and NALM6 cells stained with PBS containing 0.1 μg/mL acridine orange were evaluated for GFP and RFP channels using a fluorescent microscope (Lionheart FX Automated microscope; Agilent BioTek Instruments Inc. ...
-
bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... then washed 3 × 10minute and post-fixed with 0.1% PFA for 10minutes and mounted using fluorescent mounting medium (DAKO, USA).
-
bioRxiv - Microbiology 2023Quote: ... and the fluorescent emission (excitation at 486-580 nm and emission at 568-590 nm) was measured using Synergy HT (BioTek, Agilent). Uncoated beads and beads coated with BSA were used as controls ...
-
bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
-
bioRxiv - Cell Biology 2023Quote: ... All sections were washed three times for 3 min with PBS and mounted with Dako Fluorescent Mounting Medium (Agilent, USA).
-
bioRxiv - Neuroscience 2024Quote: ... sections were washed with PBS for 15 mins (3×5min fresh solution) and cover-slipped with fluorescent mounting medium (Dako).
-
bioRxiv - Cell Biology 2024Quote: ... 0.2% Triton X-100 and incubation with appropriate secondary antibody for 2 hr at room temperature in blocking buffer before washing and mounting in fluorescent mounting medium (DAKO). Images were acquired using a Leica SP8 confocal microscope ...
-
bioRxiv - Biochemistry 2019Quote: ... Assays were resolved by SDS-PAGE and results were analysed by western blotting with anti-ubiquitin primary antibody (Z0458, Dako) and anti-rabbit HRP secondary antibody for the unlabelled Ubc13~Ub conjugate ...
-
bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
-
bioRxiv - Molecular Biology 2022Quote: ... the blots were further incubated with a primary anti-total Tau antibody (rabbit Dako-A0024, 1:5000 in blocking buffer), then with the anti-rabbit HRP-conjugated secondary antibody ...
-
bioRxiv - Genomics 2019Quote: ... Quality and integrity of extracted RNA was checked by Northern blot and/or analysis in a RNA 6000 Pico chip in a 2100 bioanalyzer (Agilent).
-
bioRxiv - Molecular Biology 2023Quote: ... each reaction was diluted with 100μl 2X SSC and slot blotted using Bio-Rad Slot blot apparatus onto Hybond-N+ membranes (Amersham) which were UV crosslinked with autocrosslink settings (120mJ/cm2) of Stratalinker 1800 (Stratagene) apparatus ...
-
bioRxiv - Neuroscience 2019Quote: ... After one last wash with DPBS for 5 minutes at RT the coverslips were imbedded in fluorescent mounting medium (DAKO #S3023). Primary antibodies that were used ...
-
bioRxiv - Neuroscience 2021Quote: ... the slides were counterstained with DAPI for 30 sec at RT and mounted using Dako fluorescent mounting medium (Agilent Technologies, USA). The dyes used for signal detection were Opal Dye 520 and Opal Dye 570 (Akoya Biosciences ...
-
bioRxiv - Biochemistry 2022Quote: ... The fluorescent labeled oligosaccharides were separated and quantified by electrophoresis using an OLIGO Profiling Gel and a FACE imaging system (ProZyme, Inc.). The stoichiometry of labeling was such that only one molecule of fluorophore was attached to each molecule of oligosaccharide ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were washed four times with PBS containing Ca2+ and Mg2+ and mounted in a fluorescent mounting medium (DAKO, CA, USA). Finally ...
-
bioRxiv - Cell Biology 2021Quote: ... washed three times in PBST containing 10 µg/ml DAPI for 5 minutes and mounted with a coverslip in fluorescent mounting media (Dako, S3023). All images were taken using a Zeiss LSM 710 confocal microscope and quantified manually using ImageJ software ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections or cell containing coverslips were mounted on gelatin coated slides with Fluorescent Mounting Medium (S3023, Dako Cytomation, Glostrup, Denmark). The following primary antibodies were used ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were washed three times with PBS and one time with distilled water and mounted with Dako Fluorescent Mounting Medium (Dako, Denmark). Samples were visualized in the epifluorescence microscope Olympus BX53 fluorescence microscopy with UPLFLN 100× oil objective (numerical aperture 1.30) ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR reactions using SYBRgreen fluorescent reagent and/or EvaGreen™ were achieved on the Stratagene Mx3000P system (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: The above 12 OR genes linked to a wild-type internal ribosome entry site from the encephalomyocarditis virus (EMCV IRES) and the mCherry fluorescent protein gene (Shaner et al., 2004) were inserted into a modified pBlueScript KS (Agilent/Stratagene) called plasmid “L” (Zboray et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were mounted in glass slides using 0.2% gelatine in TB and cover-slipped with fluorescent mounting medium (FluorSave Tm Reagent; Dako, Glostrup, Denmark).
-
bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
-
bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR reactions employing SYBRgreen fluorescent reagent and/or EvaGreen™ were performed in the Stratagene Mx3000P system (Agilent Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed 5 x 15 min in PBST at RT and slides were mounted in DAKO fluorescent mounting medium (Agilent Technologies). EdU staining was performed on sections from larvae that received an EdU pulse ...
-
bioRxiv - Bioengineering 2024Quote: ... Fluorescence was quantified using an excitation of 530/15 nm and emission of 590/15 nm on a fluorescent plate reader (BioTek Cytation 5, Agilent Technologies).
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (#P0047; Dako Products Agilent, Santa Clara, CA, USA) or goat anti-rabbit (#A00160 ...
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... the blots were incubated with secondary antibodies: horseradish peroxidase-conjugated goat anti-mouse (#P0047; Dako Products Agilent, Santa Clara, CA, USA) or goat anti-rabbit (#A00160 ...
-
bioRxiv - Biochemistry 2023Quote: ... blots were incubated for 1h at room temperature with a horse radish peroxidase-labeled secondary antibody (anti-rabbit, Dako; 1/1000), and revealed by ECL chemiluminescence (Millipore ...
-
bioRxiv - Cell Biology 2021Quote: ... The aorta was opened in a reproducible manner by cutting along the greater curvature then mounted on glass slides with mounting medium (Dako Fluorescent; DakoCytomation).
-
bioRxiv - Neuroscience 2023Quote: ... sections were counterstained with DAPI (1:1000 in 1X PBS) for 5 min and coverslipped with fluorescent mounting medium (#S3023, DAKO, Jena, Germany). Images were obtained using an Axioplan M2 fluorescent microscope (Zeiss ...
-
bioRxiv - Neuroscience 2023Quote: ... Then the sections were mounted into microscopic slides (Epredia™ SuperFrost Plus™ Adhesion Microscopic Slides) and covered with coverslips (Eprdia Cover Slip) along with fluorescent mounting medium (DAKO). The imaging was done by inverted laser scanning confocal microscope (LSM 880 ...
-
bioRxiv - Cell Biology 2023Quote: For confocal laser scanning microscopy all images were taken from fixed specimens embedded in Dako fluorescent mounting medium (Dako Inc, Carpinteria, USA) on a LEICA SP5 confocal microscope equipped with a 63.0x/1.40 NA oil HCX PL APO CS UV objective and analyzed using LAS AF Lite software ...